Determination of demineralization depth in tooth enamel exposed to abusive use of whitening gel using micro-Energy Dispersive X ray Fluorescence

Pessanha, Sofia; Coutinho, Sara; Carvalho, Luísa; Silveira, João; Mata, António

doi:10.1016/j.sab.2017.10.001

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…In fact, in the carry out protocols (Figure ), the enamel samples were only immersed in solution with pH below the demineralizing pH of Hydroxyapatite (5.5) for 4 min and for 8 min (for Xeros™ and SST®, respectively) at a time, being most of the time immersed in saliva to recover. Moreover, previous studies with continuous acidic action on human enamel only determined significant decrease in mineralization after 1 week of treatment when using products with much lower pH (pH = 3), otherwise, no effective demineralization was seen …”

Section: Resultsmentioning

confidence: 87%

Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

Luis

Silva

Silveira

et al. 2018

J Raman Spectroscopy

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In this work, two gustatory stimulants of salivary secretion (GSSS) were studied using Raman microscopy to evaluate their potential demineralization effect in human enamel samples and proton induced gamma-ray emission (PIGE) to quantify fluorine uptake. These pharmaceutical products are usually administrated to patients who have revealed xerostomia, namely, caused by radiation therapy, chemotherapy, or diseases such as Sjögren's Syndrome. Traditional GSSS are composed of citric or malic acids that can promote enamel erosion by decreasing salivary pH below the critical level of hydroxyapatite demineralization (pH = 5.5). Recently, new GSSS composed of malic acid, fluorine, and xylitol were developed, which are supposed to decrease erosion potential risk and uptake fluorine in dental enamel. In this, in vitro study, 16 specimens of human enamel were randomized in two different groups exposed to two different GSSS: S group-SST® (with citric and malic acids, without fluorine) and X group-Xeros™ (with malic acid, fluorine, and xylitol) and analysed before and after treatment. The treatment simulated administration of four tablets per day during eight consecutive days. Statistically, significant increase in fluorine concentration was found in samples treated by Xeros™, whereas no significant differences were found in fluorine concentration for the ones treated using SST® treatment. No demineralization was found in dental enamel samples treated with either Xeros™ or SST®.

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Section: Resultsmentioning

confidence: 87%

Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

Luis

Silva

Silveira

et al. 2018

J Raman Spectroscopy

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“…The maximum intensity for these scans was compared before and after erosive challenge in order to determine the depth of demineralization. This methodology used for the depth profiling analysis is further described in Pessanha et al…”

Section: Methodsmentioning

confidence: 99%

“…In this work, the demineralization induced by Coca‐Cola® was evaluated using Raman spectroscopy and energy‐dispersive X‐ray fluorescence (EDXRF). Both analytical techniques have proven suitable for the evaluation of demineralization of human enamel by external agents or by carious lesions . To accomplish this goal, an erosive protocol was adopted for the in vitro study of the effect of this beverage in human enamel, both superficial and in‐depth.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of the demineralization induced in human enamel by an acidic beverage using X‐ray fluorescence spectroscopy and Raman microscopy

et al. 2018

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This study was conducted with the purpose of evaluating the demineralization effect of a widely consumed acidic soft drink, Coca-Cola®, in human enamel. This way, an in vitro model for the daily intake of this beverage was developed taking into account the intraoral environment. The evaluation of the enamel specimens was undertaken considering two approaches, the direct analysis of enamel surface and the study of specimens as cross sections. The depolarization ratio of the phosphate symmetric stretching band in Raman spectra was used to evaluate the loss of mineralization of the hydroxyapatite matrix, and the changes regarding the elemental content was performed using energydispersive X-ray fluorescence (EDXRF). For comparison, the Rayleigh-to-Compton ratio in EDXRF spectra of enamel samples was also determined in order to establish alterations in the average atomic number of the samples before and after erosive challenge.Considering the model applied and the timeframe of study, we determined evidences of demineralization after consumption of this drink. There was a significant increase of depolarization ratio in most of the analyzed specimens as well as a decrease of the concentration of major elements concomitant with apparent increase of the concentration of trace elements. Moreover, depths of demineralization of tens of micrometers were obtained with both spectroscopic techniques, showing consistency between the obtained results.

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Investigation of the protective suitability of a dental fluorinated varnish by means of X Ray fluorescence and Raman spectroscopy

Otel

Dias

Pereira

et al. 2022

Journal of Trace Elements in Medicine and Biology

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Determination of demineralization depth in tooth enamel exposed to abusive use of whitening gel using micro-Energy Dispersive X ray Fluorescence

Cited by 5 publications

References 12 publications

Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

In vitro study of the demineralization induced in human enamel by an acidic beverage using X‐ray fluorescence spectroscopy and Raman microscopy

Investigation of the protective suitability of a dental fluorinated varnish by means of X Ray fluorescence and Raman spectroscopy

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