Determination of eight high-intensity sweeteners in alcohol beverages by HPLC-MS/MS

Zou, Xiao; Zhang, Nini; Li, Genrong; Long, Minnan; Xiao, Zhaojing; Tong, Lanyan; Ma, Yan

doi:10.1063/1.5075664

Cited by 2 publications

(6 citation statements)

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“…The limits of detection (LOD) and quantitation (LOQ) were obtained through calculation from the calibration curves (Table V). The obtained values of LOD varying from 0.002 mg/L to 0.014 mg/L allowed the detection of trace amounts of sweeteners and are in line with the lowest values found in the literature (Zou et al, 2018;Li et al, 2020).…”

Section: Limits Of Detection and Quantitationsupporting

confidence: 88%

“…Nowadays, the most used method is reverse-phase high performance liquid chromatography (RP-HPLC) combined with different choices of detectors (Kubrica et al, 2015). Several authors reported the use of ultra-violet-visible detectors (UV-vis), diode array detector (DAD), electrochemical detector (ECD), evaporative light-scattering detection (ELSD), charged aerosol detector (CAD) and mass spectrometry (MS) (Zou et al, 2018;Li et al, 2020). In the analysis of sweeteners there are many challenges to be faced and every method or technique has its own limitations.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the use of sweeteners in wine being totally prohibited, they are often used to produce counterfeit wines (Geana et al, 2012). Besides Geana et al (2012), Zou et al (2018) reported one out of 10 wines detected with sodium cyclamate (0.28 mg/L) and neotane (0.36 mg/L), and Li et al (2020) reported five wines detected with sweeteners. Unlike the beverages in which sweetener addition is permitted and regulated, bearing in mind that wines may not contain any sweeteners, even at trace concentrations, a method to detect and quantify these compounds at extremely lower levels is necessary.…”

Section: Introductionmentioning

confidence: 99%

“…Concerning the analysis of sweeteners in wines, the literature is scarce, especially when considering simultaneous analysis of natural (e.g., stevia-based sweeteners) and synthetic high intensity sweeteners (Kubrica et al, 2015;Zou et al, 2018). Kubrica et al (2015) presented an HPLC method coupled with ESI and a triple quadrupole MS instrument for the analysis of 14 sweeteners.…”

Section: Introductionmentioning

confidence: 99%

“…Also, the validation was made with a soft drink. Zou et al (2018) used the same type of instrument to analyse eight sweeteners with very good results (limit of detection varying from 0.002 mg/L to 0.0035 mg/L) but validation was performed in beer and the study of the applicability of the method to wine is limited. Using a different approach, Li et al (2020) successfully employed a direct analysis in real time MS (DART-MS) instrument.…”

Section: Introductionmentioning

confidence: 99%

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Determination of sweeteners in wine by liquid chromatography coupled with mass spectrometry (LC/MS)

Neves

Ribeiro

Esteves

et al. 2021

Ciência Téc. Vitiv.

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Sweeteners are food additive substances that give a sweet taste to foods but their use in oenological practices is forbidden. Making use of the capabilities of liquid chromatography coupled with mass spectrometry, a method for wine analysis was developed and validated for the detection and quantitation of some of the most widely used sweeteners: aspartame, potassium acesulfame, sodium cyclamate, saccharin, sucralose and stevioside. A matrix-matched calibration was used for all compounds obtaining a linear concentration range from 50 μg/L to 1000 μg/L. The limit of detection ranged from 0.002 mg/L to 0.014 mg/L, and the limit of quantification varied between 0.005 mg/L and 0.048 mg/L. Precision and recovery were assessed for 50 μg/L, 250 μg/L and 1000 μg/L with repeatability and intermediate precision values from 0.6% to 21.6% and 2.7% to 26.4% respectively, and recoveries ranging from 60% to 126%. These results were achieved using minimal sample preparation with a fast and high throughput method that is applicable to a wide range of wine matrices.

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Section: Limits Of Detection and Quantitationsupporting

confidence: 88%