Determination of heparin using flow injection analysis with spectrophotometric detection

Němcová, I.; Rychlovský, Petr; Havelcová, Martina; Brabcová, M

doi:10.1016/s0003-2670(99)00472-9

Cited by 39 publications

(17 citation statements)

References 12 publications

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“…The most common option implies the selection of the analytical signal obtained from the detector, which is monitoring a transient concentration variation in relation to the nature of the analyte and the selected chemical system. Different detectors have been used in the analysis of pharmaceuticals by FIA making it possible to consider different responses such as the absorbance in the UV/Vis region [11,12,13,14], the chemiluminescence intensity [15,16,17] or the phosphorescence intensity [18]. Also, different analytical signals have been used, such as the peak height [19,20,21,22,23,24,25] or the residence time [21].…”

Section: Selection Of the Responsementioning

confidence: 99%

An overview of qualimetric strategies for optimisation and calibration in pharmaceutical analysis using flow injection techniques

Bosque-Sendra

Gámiz‐Gracia

Garcı́a-Campaña

2003

Analytical and Bioanalytical Chemistry

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Flow Injection analysis represents an attractive tool because of its great advantages, such as versatility, speed, high sampling rate and wide applicability in the field of pharmaceutical analysis. However, due to the inherent characteristics of the technique, the choice of the best set of operational and chemical conditions is complicated and the conventional univariate optimisation method present some limitations, mainly due to fact that the interdependence of variables is not considered. In relation to the calibration process, because of the transient character of the signals obtained using FIA manifolds coupled with different detection techniques, different strategies can be used in calibration to solve some problems related to the nature of the signal thereby improving the performance characteristics of the method. This paper offers an overview of different methodologies used in optimisation based on the use of statistically designed experiments and some strategies developed for calibration applied to the analysis of pharmaceuticals.

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Section: Selection Of the Responsementioning

confidence: 99%

An overview of qualimetric strategies for optimisation and calibration in pharmaceutical analysis using flow injection techniques

Bosque-Sendra

Gámiz‐Gracia

Garcı́a-Campaña

2003

Analytical and Bioanalytical Chemistry

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“…3 So Hep level in the patient's blood need to be carefully and accurately monitored during surgery and recovery. The reported methods of Hep determination focused on: Flowing injection analysis, 4 capillary chromatography, 5 surface plasmon resonance sensor analysis, 6 membrane electrode via protamine titration 7 and extracorporeal membrane oxygenation. 8 The methods mentioned above have high fluorescence quantum yield, large stokes shift, narrow emission bands and a large fluorescence lifetime.…”

Section: Introductionmentioning

confidence: 99%

Determination of Heparin Using Norfloxacin-cerium Complex as a Fluorescence Probe by Spectrofluorimetry

Patil¹,

Mote²,

Patil³

et al. 2009

Bulletin of the Korean Chemical Society

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A simple, rapid, practical and sensitive spectofluorimetric method was developed for the determination of trace amount of heparin (Hep). Under the Optimum conditions, we studied the interaction between NFLX-Ce 3+ -Hep complex by using absorption and fluorescence spectra. It was observed that Hep remarkably enhance the fluorescence intensity of the NFLX-Ce 3+ complex at λ = 356 nm in the buffer solution of pH = 7.60 and the enhancement effect is shown to relate with the concentration of Hep. The linear range and detection limit for the determination of Hep was obtained. By the Rosenthal graphic method, the association constant (K) and binding numbers (N) of Hep with probe were investigated. This method is relatively free of interference from coexisting substances and successfully applied for the determination of heparin in heparin sodium injection samples. A suitable mechanism of fluorescence enhancement between NFLX-Ce 3+ and the NFLX-Ce 3+ -Hep systems were proposed and discussed.

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“…At present many methods had been proposed for the determination of heparin including spectrophotometry [1 -4], fluorometry [5], HPLC [6], electrophoretic method [7], flow injection analysis [8], light scattering technique [9 -11] and electroanalytical method [12 -14]. Liu et al [9 -11] applied resonance Rayleigh scattering technique to the heparin determination based on the interaction of some basic diphenylnaphthylmethane dyes such as Victoria blue 4R, Victoria blue B, which could result in a significant enhancement of the resonance Rayleigh scattering response.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Heparin Based on Its Interaction with Light Green

Sun¹,

Ding²,

Wang³

et al. 2006

Electroanalysis

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The determination of heparin with linear sweep voltammetry has been developed in this paper based on its interaction with light green (LG). In pH 2.0 Britton -Robinson (B -R) buffer solution, LG has a sensitive linear sweep voltammetric reduction peak at À 0.41 V (vs. SCE) and heparin can react with LG to form an ion-association electroactive complex, which resulted in the decrease of the peak current of the reaction solution. Under the selected experimental conditions the decrease of peak current was directly proportional to the concentration of heparin in the range of 0.8 -20.0 mg/mL with the linear regression equation as Di '' p (nA) ¼ 79.12 C À 101.44 (n ¼ 14, g ¼ 0.996) and the detection limit as 0.28 mg/mL (3s). The effects of coexisting substances on the determination of heparin were investigated and the results showed that this method had good selectivity. The method was further applied to the determination of heparin content in heparin samples with satisfactory results and good recovery.

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Determination of heparin using flow injection analysis with spectrophotometric detection

Cited by 39 publications

References 12 publications

An overview of qualimetric strategies for optimisation and calibration in pharmaceutical analysis using flow injection techniques

An overview of qualimetric strategies for optimisation and calibration in pharmaceutical analysis using flow injection techniques

Determination of Heparin Using Norfloxacin-cerium Complex as a Fluorescence Probe by Spectrofluorimetry

Electrochemical Detection of Heparin Based on Its Interaction with Light Green

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