Determination of IgG levels in bovine bulk milk samples from different regions of Spain

Conesa, Celia; Lavilla, María; Sánchez, Lourdes; Pérez, María Dolores; Mata, Luis; Razquín, Pedro; Calvo, Miguel

doi:10.1007/s00217-004-1016-0

Cited by 20 publications

(21 citation statements)

References 13 publications

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“…This is in agreement with Conesa et al (2005), who reported that IgG values from the whole population of analysed samples were found to be significantly higher also in the spring. In another study, the seasonal effect was the only significant variation factor observed, with the highest values in the spring for LF and in the winter for IgG (Konuspayeva et al 2007).…”

Section: Discussionsupporting

confidence: 93%

Concentration of lactoferrin and immunoglobulin G in cows’ milk in relation to health status of the udder, lactation and season

Musayeva¹,

Sederevičius²,

Želvytė³

et al. 2016

Polish Journal of Veterinary Sciences

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The aim of this study was to analyze an effect of udder health status, somatic cell count (SCC), stage and number of lactations, and different seasons on the concentration of lactoferrin (LF) and immunoglobulin G (IgG) in quarter milk samples (n=120) from crossbreed (Lithuanian Black-and-White & Holstein) dairy cows. Quarter health status was based on SCC and microbiological analysis. The highest mean value of LF and IgG were observed in quarters with subclinical mastitis 0.1 ± 0.02 mg/ml and 0.41 ± 0.06 mg/ml, respectively. Grouping the data according to SCC revealed increased LF (0.07 ± 0.01 mg/ml as against 0.06 ± 0.01 mg/ml) and IgG values (0.27 ± 0.05 mg/ml as against 0.23 ± 0.02 mg/ml) in DQ (SCC from 201,000 ≥ 401,000 cells/ml) compared to HQ (SCC up to 200,000 cells/ml). The milk LF and IgG levels were effected by stage of lactation (p<0.01 and p<0.05, respectively) and season of the year (p<0.001 and p<0.001, respectively). Nevertheless, SCC and subsequent lactation (p>0.05) had no effect on these immunity components.

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Section: Discussionsupporting

confidence: 93%

Concentration of lactoferrin and immunoglobulin G in cows’ milk in relation to health status of the udder, lactation and season

Musayeva¹,

Sederevičius²,

Želvytė³

et al. 2016

Polish Journal of Veterinary Sciences

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“…In our case, however, all the samples belonged to the mature lactation period, during which IgG concentration may either increase or decrease depending on different factors, such as the time of year, the animal’s physiological status, feeding [59,60]; for example, the concentration increases when inflammatory conditions are present, such as mastitis [54,57,58,61]. Such a circumstance could for instance account for the IgG values found by us between day 39 and day 148 of lactation.…”

Section: Resultsmentioning

confidence: 99%

Determination of Lactoferrin and Immunoglobulin G in Animal Milks by New Immunosensors

Campanella

Martini

Pintore

et al. 2009

Sensors

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Two different immunosensors, recently developed for the determination of antibacterial proteins (lactoferrin and immunoglobulin G) in buffalo milk and in other commercial animal milks samples, were used in the present study. The aim was to propose these immunosensor methods for routine control of important diet products, such as cow and goat milks, and in particular buffalo milk. To this end we employed two different kinds of immunosensors: one for the analysis of immunoglobulin G (IgG), the other was a new amperometric immunosensor for lactoferrin analysis. Lactoferrin and IgG immunosensors were also used for the determination of lactoferrin and immunoglobulin G in buffalo milk on different days of lactation.

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“…Somatic cell counts were made by DeLaval Cell Counter® (DeLaval International, Sweden) cell counter device (Smith, 1996). IgG measurements were performed using a commercial Bovine IgG ELISA Test package (Cusabio Biotech Co., Ltd.) and an ELISA reader (Biotek ELX800) (Conesa et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…Dead Corynebacterium cutis bacteria are easily phagocytized by the macrophages when administered to the organism and then stimulates the release of TNF, IL-1 and IL-6 from the macrophages (Shalaby et al, 1992). These cytokines generates a secondary effect on lymphocyte function and provides the release of IL-2 and IFN-γ, therefore the reactivation of the immune system (Conesa et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The efficiency of Corynebacterium cutis lysate in cows with subclinical mastitis

2016

Res. Opin. Anim. Vet. Sci.

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In this study, it was aimed to determine the therapeutic efficiency of Corynebacterium cutis lysate that is a nonspecific immunostimulant in cows with subclinical mastitis. The study material was 88 udder lobs with subclinical mastitis of cows from different breeds and age (4-7 years) based on the same farm. Subclinical mastitis was diagnosed by microbiological tests, California Mastitis Test (CMT) and Somatic Cell Count (SCC). 10 ml of Corynebacterium cutis lysate was injected subcutaneously to the animals which were diagnosed with subclinical mastitis. After 15 days from the injection microbiological tests, CMT and SCC determinations were performed in the same udder lobs. Additionally, milk samples were taken from the said udder lobs before and after Enzyme-Linked Immunosorbent Assay (ELISA) determined immunoglobulin G (IgG) concentrations. CMT positivity rates, SCC and IgG levels before and after Corynebacterium cutis lysate injections were 100% and 63.63%; 897.470 and 923.780 cell/ml; 845.11 and 887.27 mg/ml, respectively. The microbiological growth rates before and after the applications were found to be 13.63% and 22.73%, respectively. The obtained data showed that, although not statistically significant, microbiological growth rates and SCC were increased after Corynebacterium cutis lysate application to the cows with subclinical mastitis. However, it was observed that the milk IgG levels were increased significantly after the application. These results may indicate the use of Corynebacterium cutis lysate applications as a supportive treatment to enhance the resistance in cows against subclinical mastitis.

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Determination of IgG levels in bovine bulk milk samples from different regions of Spain

Cited by 20 publications

References 13 publications

Concentration of lactoferrin and immunoglobulin G in cows’ milk in relation to health status of the udder, lactation and season

Concentration of lactoferrin and immunoglobulin G in cows’ milk in relation to health status of the udder, lactation and season

Determination of Lactoferrin and Immunoglobulin G in Animal Milks by New Immunosensors

The efficiency of Corynebacterium cutis lysate in cows with subclinical mastitis

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