Determination of Indole Alkaloids by High-Performance Liquid Chromatography with Resonance Rayleigh Scattering Detection

He, Yuting; Peng, Jingdong; Tang, Jinxia; Zhou, Mingqiong; Zhang, Cheng

doi:10.1080/00032719.2014.986678

Cited by 4 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection limits of ajmalicine, reserpine, and deserpidine were found to be 0.057, 0.023, and 0.016, respectively. 61 Liu et al developed a new two-dimensional liquid chromatography (LC) method for the simultaneous estimation of three monoterpene indole alkaloids in urine, tissue, and plasma samples from Gelsemium elegans. The outcome of this research showed that this method exhibited favorable quantification capabilities compared to a previously developed HPLC method.…”

Section: Analytical Approaches For Estimation Of Alkaloidsmentioning

confidence: 99%

See 1 more Smart Citation

An insight into recent updates on analytical techniques for bioactive alkaloids

Saini,

Seni,

Chawla

et al. 2024

Phytochemical Analysis

View full text Add to dashboard Cite

IntroductionAlkaloids represent a wide class of naturally existing nitrogen‐containing organic compounds having diverse biological activities. They are primary bioactive substances extracted from diverse plant parts. Due to their diverse biological activities, they are frequently used as medicines. The alkaloids have diverse pharmacological impacts on the human body; alkaloids are used for prevention, treatment, and reduction of discomfort associated with chronic illnesses. As most alkaloids exist in plants in complex form, combined with numerous other natural plant components, it is essential to recognize and characterize these molecules using different analytical techniques.ObjectivesWe aimed to review the literature on the methods and protocols for the analysis of naturally occurring alkaloids.MethodsWe carried out a literature survey using the PubMed, Scopus, and Google Scholar databases and other relevant published materials. The keywords used in the searches were “alkaloids,” “analytical methods,” “HPLC method,” “GC method,” “electrochemical methods,” and “bioanalytical methods,” in various combinations.ResultsIn this article, several classes of alkaloids are presented, along with their biological activities. Moreover, it includes a thorough explanation of chromatographic techniques, hyphenated techniques, electrochemical techniques, and current trending analytical methods utilized for the isolation, identification, and comprehensive characterization of alkaloids.ConclusionsThe various analytical techniques play an important role in the identification as well as the characterization of various alkaloids from plants, plasma samples, and urine samples. The hyphenation of various chromatographic techniques with mass spectrometry and NMR spectroscopy plays a crucial role in the characterization of unknown compounds.

show abstract

Section: Analytical Approaches For Estimation Of Alkaloidsmentioning

confidence: 99%

“…The detection limits of ajmalicine, reserpine, and deserpidine were found to be 0.057, 0.023, and 0.016, respectively. 61 …”

Section: Analytical Approaches For Estimation Of Alkaloidsmentioning

confidence: 99%

An insight into recent updates on analytical techniques for bioactive alkaloids

Saini,

Seni,

Chawla

et al. 2024

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…However, RRS methods suffer from poor stability and poor selectivity due to interferences caused by complex mixtures. So, a reversed phase high-performance liquid chromatography-resonance Rayleigh scattering (RP-HPLC-RRS) method that combines the high selectivity of RP-HPLC and the high sensitivity of RRS, has been applied to detect aminoglycosides (18,19), α 1 -adrenoceptor antagonists (20), fluoroquinolones (21), proteins (22), four tetracycline antibiotics (23) and indole alkaloids (24).…”

Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh scattering technique as a detection method for the RP‐HPLC determination of local anaesthetics in human urine

et al. 2016

Self Cite

View full text Add to dashboard Cite

A highly selective and sensitive method of reversed phase high-performance liquid chromatography (RP-HPLC) coupled with resonance Rayleigh scattering (RRS) was developed for the determination of procaine, bupivacaine and tetracaine. Separation of three local anaesthetics was achieved at 35 °C on a C column. The mobile phase was 30: 70 (v/v) acetonitrile/triethylamine-phosphoric acid buffer (pH 2.9) at flow rate of 0.3 mL/min. The RRS detection was conducted by taking advantage of the strong RRS enhancement of the local anaesthetics with erythrosine reaction in an acidic medium. Under optimum conditions, the limit of detection (S/N = 3) values were in the range of 2.4-11.2 ng/mL. Recoveries from spiked human urine samples were 95.8%-104.5%. The proposed method applied to the determination of local anaesthetics in human urine achieved satisfactory results. In addition, the mechanism of the reaction is fully discussed. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

“…[27] When the wavelength of the scattered peaks of the RRS spectral band is close to the molecular absorption band, resonance occurs, making the intensity of RRS stronger compared with that of a single RS. To date, the RRS method has been widely used for the determination of trace substances, for example, nucleic acids, [28] proteins, [29] inorganic ions, [30,31] organic compounds, [32,33] drugs, [34][35][36] etc. [37][38][39] Recent studies have shown that the RRS method can be also applied to the determination of antibiotics such as fluoroquinolones, [40,41] cephalosporins, [42] tetracyclines, [43] macrolides, [44] aminoglycosides, [45] etc.…”

mentioning

confidence: 99%

Applying resonance Rayleigh scattering and spectrofluorimetric techniques for the selective determination of neomycin sulfate using Congo red as a probe: Applications for wastewater analysis

et al. 2022

View full text Add to dashboard Cite

Two simple, sensitive, and low‐cost fluorescence spectroscopy methods for neomycin (NEO) detection were developed. Both methods were based on the interaction between NEO and Congo red (CR) in acidic buffer medium to form an ion‐association complex. The quenching effect of the formed ion‐association complex on the fluorescence of CR at 421 nm is a basic principle of fluorescence analysis, whilst the resonance Rayleigh scattering (RRS) method was used to enhance the resonance Rayleigh scattering spectrum at 384 nm by adding NEO. Experimental conditions such as pH, temperature, reaction time, CR concentration, and the ionic strength of the two methods were investigated and optimized. In addition, the effect of common coexisting substances on the method was tested and the results showed good selectivity. The composition of ion‐association complexes, the reaction mechanism, and reasons for the enhanced intensity of RRS are discussed. Under optimum conditions, the responses of the fluorescence spectrophotometry and RRS methods showed good linearity with NEO concentrations in the range 0.2–3.0 μg ml−1 and 0.1–3.0 μg ml−1, respectively. The detection limits of fluorescence spectrophotometry and RRS spectroscopy techniques were 0.02 μg ml−1 and 0.01 μg ml−1, respectively. Finally, the two methods were applied to the analysis of wastewater and the results were satisfactory.

show abstract

Determination of Indole Alkaloids by High-Performance Liquid Chromatography with Resonance Rayleigh Scattering Detection

Cited by 4 publications

References 43 publications

An insight into recent updates on analytical techniques for bioactive alkaloids

An insight into recent updates on analytical techniques for bioactive alkaloids

Resonance Rayleigh scattering technique as a detection method for the RP‐HPLC determination of local anaesthetics in human urine

Applying resonance Rayleigh scattering and spectrofluorimetric techniques for the selective determination of neomycin sulfate using Congo red as a probe: Applications for wastewater analysis

Contact Info

Product

Resources

About