Determination of ochratoxin A in wine from the southern region of Brazil by thin layer chromatography with a charge-coupled detector

Abstract

The presence of ochratoxin A (OTA) is evaluated using thin layer chromatography (TLC) with a charge-coupled detector (CCD) in 88 red wine samples (2009 vintage) from Brazil. Samples included 75 wines from Rio Grande do Sul, nine from Santa Catarina and four from Paraná state; 56 wines were Cabernet Sauvignon and 32 were Merlot varieties. The method included the analysis of fluorescent images, acquired under UV light, using ImageJ software. Mean recovery of OTA was 82.3% using immunoaffinity columns (IAC). The limits of quantification (LOQ) and detection (LOD) were 0.8 and 0.2 µg l(-1), respectively. Results showed a 5.7% contamination of OTA, with five positive samples--three Cabernet Sauvignon and two Merlot. Santa Catarina and Paraná showed one positive sample each, although the concentrations were below the limit of quantification. The highest concentration found was 0.84 µg l(-1) in one sample from Rio Grande do Sul.

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

“…Various detection methods had been developed to analyze the level of OTA contamination in food products. Previous studies using thin layer chromatography (TLC) to detect OTA in 88 wine samples from Brazil determined that 5.7% of the samples were contaminated with OTA, and the maximum concentration of OTA was 0.84 ng mL −1 13 . Mariño‐Repizo et al .…”

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

“…TLC equipment is simple and easy to operate, but there are many factors that affect reproducibility and precision. Using TLC, the OTA content in wine products was detected within 2 μg/L, which is the legal limit for OTA in wine stipulated by the European Commission (Teixeira et al, 2011 ). RP-TLC can be used confirm results obtained via normal phase TLC (Valenta, 1998 ).…”

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

“…At present, the technology of OTA analysis mainly involves traditional analytical techniques, such as thin-layer chromatography (TLC) [ 7 ], high-performance liquid chromatography (HPLC), gas chromatography (GC), and liquid chromatography tandem mass spectrometry (LC-MS/MS) [ 8 , 9 , 10 ]. Although these methods have good accuracy and reproducibility, the requirements of professional operators, expensive equipment, cumbersome sample pretreatment processes, or long processing times have limited their practical applications.…”