Determination of olanzapine in plasma by high-performance liquid chromatography using ultraviolet absorbance detection

Dusci, L.J.; Hackett, L.P.; Fellows, Linda M.; Ilett, Kenneth F.

doi:10.1016/s1570-0232(02)00164-2

Cited by 79 publications

(55 citation statements)

References 14 publications

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“…Furthermore, the methods are more sensitive, as compared to many published spectrophotometric metho-ds (Jasinska, Nalewajko., 2004;Mohamed., 2008;Krebs et al, 2006) and also some HPLC methods (Olesen et al, 1998;Harald et al, 2001;Concetta et al, 2006;Dusci et al, 2002;Olesen et al, 2001;Shah et al, 2007;Reddy et al, 2007;Xuejun et al, 2004). The methods are simpler as compared to published methods (Jasinska, Nalewajko, 2004;Mohamed, 2008;Krebs et al, 2006) with respect to experimental conditions, instrumental setup and the stability of the measured species.…”

Section: Resultsmentioning

confidence: 99%

“…Several analytical methods have been reported for the determination of OLP in biological fluids and in pharmaceutical preparations, and they include high performance liquid chromatography (HPLC) with ultraviolet (Olesen et al, 1998;Boulton et al, 2001;Harald et al, 2001;Concetta et al, 2006;Dusci et al, 2002;Olesen et al, 2001;Saracino et al, 2007;Shah et al, 2007;Reddy et al, 2007;Xuejun et al, 2004); electrochemical (Saracino et al, 2006;Bao, Potts., 2001;Kasper et al, 1999) or mass spectrometric (Thieme, Sachs., 2003;Berna et al, 2002;Titier et al, 2003;Dusci et al, 2002) detection, gas chromatography (Sabbioni et al, 2004), capillary zone electrophoresis , UVspectrophotometry and non-aqueous titrimetry (Firdous et al, 2005). However, there are only three reports on the visible spectrophotometric methods (Jasinska, Nalewajko, 2004;Mohamed, 2008;Krebs et al, 2006) based on redox reaction using hexacyanoferrate (III), cerium (IV), Nbromosuccinimide (NBS) or potassium iodate.…”

Section: Introductionmentioning

confidence: 99%

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Determination of olanzapine by spectrophotometry using permanganate

Rajendraprasad

Basavaiah

2009

Braz. J. Pharm. Sci.

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Two new spectrophotometric methods using permanganate as the oxidimetric reagent for the determination of olanzapine (OLP) were developed and validated as per the current ICH guidelines. The methods involved the addition of known excess of permanganate to OLP in either acid or alkaline medium followed by the determination of unreacted permanganate at 550 nm (method A) or bluish-green color of manganate at 610 nm (method B). The decrease in absorbance in method A or increase in absorbance in method B as a function of concentration of OLP was measured and related to OLP concentration. Under optimized conditions, Beer's law was obeyed over the ranges 2.0 to 20 and 1.0 to 10 µg mL . The LOD and LOQ for method A were calculated to be 0.37 and 1.13 µg mL -1 and the corresponding values for method B were 0.16 and 0.48 µg mL -1 . Intermediate precision, expressed as RSD was in the range 0.51 to 2.66 %, and accuracy, expressed as relative error ranged from 0.79 to 2.24 %. The proposed methods were successfully applied to the assay of OLP in commercial tablets with mean percentage recoveries of 102 ±1.59 % (method A) and 101 ±1.53 % (method B). The accuracy and reliability of the methods were further confirmed by performing recovery tests via standard addition procedure.Uniterms: Olanzapine/determination. Spectrophotometry. Permanganate. Pharmaceuticals. Dois métodos espectrofotométricos novos, usando o permanganato como o reagente oxidimétrico para a determinação da olanzapina (OLP) foram utilizados e validados de acordo com as diretrizes atuais do ICH. Os métodos envolveram a adição de excesso conhecido de permanganato à OLP em meio ácido ou alcalino, determinando-se o permanganato que não reagiu em 550 nm (método A), ou pela cor verdeazulada do manganato a 610 nm (método B). A diminuição da absorbância no método A ou o aumento da absorbância no método B, em função da concentração de OLP, foi medida e relacionada à concentração de OLP. Sob condições otimizadas, a lei de Beer foi obedecida, nas faixas de concentração de 2,0 a 20 e 1,0 a 10 ao µg mL-1, no método A e no método B, respectivamente. Os valores de absortividade molar foram de 1,34 x 104 e 2,54 x 104 l mol-1cm-1 para o método A e para o método B, respectivamente, e as sensibilidades respectivas de Sandell foram de 0,0233 e 0,0123 µg cm-2. Os LOD e os LOQ para o método A calculados foram 0,37 e 1,13 mg mL-1e os valores correspondentes para o método B foram 0,16 e 0,48 µg mL-1. A precisão intermediária, expressa como RSD, encontrou-se na faixa de 0,51 a 2,66%, e a exatidão, expressa como o erro relativo, variou de 0,79 a 2,24%. Os métodos propostos foram aplicados com sucesso ao ensaio de OLP em comprimidos comerciais, com porcentagens médias de recuperação de 102± 1,59% (método A) e de 101± 1,53% (método B). A exatidão e a confiabilidade dos métodos foram confirmadas executando testes de recuperação através de procedimento padrão de adição.Uniterms: Olanzapina/determinação. Espectrofotometria. Permanganato. Produtos farmacêuticos.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of olanzapine by spectrophotometry using permanganate

Rajendraprasad

Basavaiah

2009

Braz. J. Pharm. Sci.

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“…Results showed that extraction recovery was > 82 % for all observed concentration in rat plasma which is comparable with SPE method [8,14]. Ascorbic acid was not added to the plasma samples as addition of ascorbic acid does not affect the stability of olanzapine during storage and extraction [12]. All the stability parameters were found to meet the acceptance criteria of FDA's Guidance for Industry for Bioanalytical Method Validation 2013 [13].…”

Section: Discussionmentioning

confidence: 97%

“…Quantification of olanzapine in micro-sample rat plasma was carried out using a modified HPLC method according to previously published HPLC method by Dusci et al [12]. Olanzapine was extracted from the rat plasma samples by using liquid-liquid extraction.…”

Section: Sample Preparationmentioning

confidence: 99%

Development and Validation of Reverse Phase High Performance Chromatography Method for Determination of Olanzapine in Microsample Rat Plasma: Application to Preclinical Pharmacokinetic Study

Pervaiz¹,

Ahmad²,

Minhas³

et al. 2015

Trop. J. Pharm Res

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Purpose: To develop a sensitive and validated reverse phase-high performance liquid chromatographic (RP-HPLC) method for quantification of olanzapine in micro-sample of rat plasma using UV detection. Methods:A single oral dose of olanzapine (7 mg/kg) was given to overnight fasted rats (n = 6). Rat plasma samples containing the drug were extracted by liquid-liquid extraction using a combination of dichloromethane : n-hexane (80:20). A reverse phase chromatographic column C18 hypersil-BDS was used for chromatographic separation with a mobile phase consisting of 50 mM phosphate buffer pH 5.5, acetonitrile and methanol (50:30:20, v/v/v)

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“…Since its introduction in a therapy of psychiatric disorders in 1997, the need for reliable, sensitive and fast methods for its analysis in bulk samples and pharmaceutical preparations is obvious. Several methods have been already reported for the determination of olanzapine (OLA), including hyphenated techniques: HPLC-MS, 1,2 HPLC-UV, [3][4][5][6][7][8][9] GC-MS 10 and electrochemical. 6 There are not many spectrophotometric methods to determine OLA, based on its reaction of oxidation.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Olanzapine by Its Oxidation with N-Bromosuccinimide and Cerium(IV)sulfate

Krebs

Starczewska

Puzanowska-Tarasiewicz

et al. 2006

ANAL. SCI.

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Three simple spectrophotometric methods have been described for the assay of olanzapine in its pure and pharmaceutical formulations. The direct method (A) is based on the drug oxidation with excess of N-bromosuccinimide in acidic medium and the two indirect methods (B and C) are based on the oxidation of the drug with excess of Nbromosuccinimide and cerium(IV)sulfate, followed by the reaction of the unconsumed oxidants with celestine blue. The calibration graphs were linear over the range 10 -120 μg mL -1 (method A), 0.5 -6.0 μg mL -1 (method B) and 0.6 -3.0 μg mL -1 (method C). After validation, the proposed methods were successfully applied to assay of olanzapine in its commercial tablets with mean percentage recoveries of 101.23 ± 0.10, 96 ± 0.10 and 94 ± 0.04%. The mechanism of olanzapine oxidation with N-bromosuccinimide was also proposed.

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Determination of olanzapine in plasma by high-performance liquid chromatography using ultraviolet absorbance detection

Cited by 79 publications

References 14 publications

Determination of olanzapine by spectrophotometry using permanganate

Determination of olanzapine by spectrophotometry using permanganate

Development and Validation of Reverse Phase High Performance Chromatography Method for Determination of Olanzapine in Microsample Rat Plasma: Application to Preclinical Pharmacokinetic Study

Spectrophotometric Determination of Olanzapine by Its Oxidation with N-Bromosuccinimide and Cerium(IV)sulfate

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