Determination of Oligoelements Content of Plant Material and Assessment of Bioactive Compounds from Calendula officinalis Lyophilized Extract

Abstract

The marigold extracts are used in traditional medicine for its anti-inflammatory, decongestive, antiseptic, antifungal, antimicrobial and immunostimulating properties. Existing studies have partially proved these properties, but are incomplete with regard to the possibility of standardizing the extract using the best working conditions so as it to be included in dietary supplements. The purpose of this study is to investigate the chemical composition of the marigold extracts, their contents in flavonoids and polyphenolic compounds, as well as to determine the contents in oligoelements of the plant material through the ICP-OES method. Based on the results of this study we conclude that a complete extraction may be accomplished after lyophilizing the solution of hydroalcoholic extract, followed by extraction in acid medium so as to permit a better assessment of the bioactive compounds.

Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

“…The ABTS value was obtained using the following equation based on the calibration curve: y = 1629 x + 98.94 (R 2 = 0.998), where x = absorbance and y= µmol Trolox equivalent [32,33]. Trolox was used as a standard solution, the calibration curve was made for concentrations between 0 and 300 µM, having a correlation coefficient R 2 = 0.9956 and the regression equation (y =0.0017x+0.0848), where x = absorbance registered at 595 nm and y = µmol Trolox equivalent [34][35][36].…”

The Study of Antioxidant Capacity in Extracts from Vegetal Sources with Hypoglycaemic Action

“…As standards were used metallogenic solutions of caffeic acid, having concentrations between 1 and 3 µg/mL), processed similarly to the samples. Absorbance was measured at 715 nm, read after 2 minutes of rest, in triplicate [19,[24][25][26][27][28][29]. The calibration curve ( fig.…”

Evaluation of Phenolic Compounds Content and Antioxidant Capacity of Some Medicinal Plants, Potentially Used for Their Preventive Role Against Various Oral Diseases

“…After the addition of 100 µL of sample to 2900 µL ABTS + solution, the mixture was monitored spectrophotometrically at 734 nm. The calibration curve was made with the Trolox standard [15,16,26,[38][39][40].The calculation formula is the same as for the DPPH test.The reduction of The determination of total flavonoid contents The content of total flavonoids was determined by using a colorimetric method which has been described previously [25][26][27][28]. 1 mL of the sample (containing 0.1 mg / mL dry weight) is mixed with 4 mL water and placed in a 10 mL volumetric flask.…”

“…Recent studies on antioxidants have demonstrated their important role in offset this imbalance [10,11]. Many antioxidant compounds possess antiinflammator y, antiatherosclerotic [12], antiproliferative, antitumor [13], antimutagenic, anticarcinogenic, antibacterial or antiviral activities [13][14][15][16][17][18]. An important group of antioxidants are phenolic compounds [10,12].…”