Determination of Organic Nitrogen. Control of Variables in the Use of Nessler's Reagent

Thompson, John; Morrison, George R.

doi:10.1021/ac60056a029

Cited by 137 publications

(33 citation statements)

References 9 publications

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“…2.1. The amount of active enzyme on the sample was estimated using Nessler's reagent (mercuric potassium iodide) assay [15]. This reagent reacts with ammonia, the product of urea hydrolysis, to form a yellow adduct which absorbs at 500 nm.…”

Section: Effect Of Urea Hydrolysis On Resonance Frequencymentioning

confidence: 99%

Dynamic response of polysilicon microcantilevers to enzymatic hydrolysis of urea

Fernandez

Bhattacharya

Chadha

2010

Int J Adv Eng Sci Appl Math

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Micromolar levels of urea [1-20 mM] on surface micromachined polysilicon cantilever beams immobilized with the enzyme urease was studied. Urea hydrolyses to ammonia and carbon dioxide which are volatile in nature. Monitoring the change in resonance frequency of a urease immobilized cantilever beam during the course of urea hydrolysis, mediated by the immobilized enzyme can give us information on the reaction rates. The change in the resonance frequency of the cantilever beams as a result of urea hydrolysis was measured using Doppler Vibrometry. The reaction kinetics of the enzyme urease was studied by analyzing the dynamic response of the enzyme immobilized microcantilevers for various concentrations of urea.

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Section: Effect Of Urea Hydrolysis On Resonance Frequencymentioning

confidence: 99%

Dynamic response of polysilicon microcantilevers to enzymatic hydrolysis of urea

Fernandez

Bhattacharya

Chadha

2010

Int J Adv Eng Sci Appl Math

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“…Amino acids were products of Ajinomoto Co., Tokyo, Japan. Methanethiol and ammonia were determined with N,N-dimethyl-p-phenylene diamine.HC1 [ 11 ] and Nessler reagent [ 12], respectively.…”

Section: North-holland Publishing Company -Amsterdammentioning

confidence: 99%

Purification and properties of methioninase from Pseudomonas ovalis

et al. 1976

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“…L-Methioninase activity was assayed by direct Nesslerization according to the method of Thompson and Morrison (1951) with some modifications. The standard reaction system contains 1 ml of 1% L-methionine in phosphate buffer (pH 7.0), 0.1 ml of pyridoxal phosphate, and 1 ml of crude enzyme.…”

Section: Methioninase Assaymentioning

confidence: 99%

Isolation, screening and statistical optimizing of L-methioninase production by Chaetomium globosum

Hamed¹,

Elsoud²,

Mahmoud³

et al. 2016

Afr. J. Microbiol. Res.

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Egyptian soil sample was screened for isolation methioninlytic fungi by a rapid plate assay procedure. Eighteen strains of different isolated fungi were screened quantitavely for their L-methioninase activity. Chaetomium globosum was the most efficacious isolate and a dematiaceous filamentous fungi, it was identified at the molecular level by ribotyping 18S rRNA along with the biochemical characterization, and lastly completed by BLAST analysis by structure of a phylogenetic tree. Results showed that, the optimum levels of the incubation period, temperature, pH, methionine, sucrose and sodium nitrate concentrations were 3 days, 30°C, 7, 0%, 30 g/l and 1g/l, respectively. According to the produced model, at these levels, C. globosum produce L-methioninase with predicted specific activity of (≈2225 U/mg); so L-methioninase could be a good source for clinical therapeutic application. The rRNA sequence of C. globosum was deposited to gene bank under accession number KXO24450

show abstract

Determination of Organic Nitrogen. Control of Variables in the Use of Nessler's Reagent

Cited by 137 publications

References 9 publications

Dynamic response of polysilicon microcantilevers to enzymatic hydrolysis of urea

Dynamic response of polysilicon microcantilevers to enzymatic hydrolysis of urea

Purification and properties of methioninase from Pseudomonas ovalis

Isolation, screening and statistical optimizing of L-methioninase production by Chaetomium globosum

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