Determination of Phosphorus

Ueda, Tadaharu

doi:10.2116/analsci.highlights2006

Cited by 4 publications

(2 citation statements)

References 15 publications

(15 reference statements)

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“…Because the 31 P nucleus can be a selective marker of nucleic acids and other phosphorylated compounds, its observation is expected to provide useful information in this context, and 31 P NMR spectroscopy can be a powerful tool for determination of phosphorus. 7 Histone proteins provide critical structural support in the formation of chromosomes. Long DNA molecules need to fit into the cell nucleus, and they wrap around complexes of histone octamers in order to form more compact chromosomal shapes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Binding Mode of 2'-GMP to Proteins Using 1H/31P NMR Spectroscopy

et al. 2020

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1 H/ 31 P NMR techniques were applied to analyze the binding mode of guanosine 2'-monophosphate (2'-GMP) to histone. To date no structures of the complex comprising 2'-GMP and histone have been deposited in Protein Data Bank. Because 31 P nucleus can be a selective marker of phosphorylated compounds, the combined use of 1 H and 31 P NMR spectroscopy has been applied to investigate these molecular interactions. The complex formation was initially confirmed by 31 P-diffusion ordered spectroscopy and 31 P-T1 measurements. In the 1 H{ 1 H} saturation transfer difference experiments, H2' and H3' signals of 2'-GMP were significantly attenuated, while the rest of the unexchangeable protons were observed, indicating that contribution of H2' and H3' to the binding epitopes was low. The WaterLOGSY type experiment with 31 P detection also indicated that a phosphorylated group located close to H2' and H3' had little access to histone.

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Section: Introductionmentioning

confidence: 99%

Analysis of Binding Mode of 2'-GMP to Proteins Using 1H/31P NMR Spectroscopy

et al. 2020

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“…as the molybdenum yellow method and molybdenum blue method, respectively, and have been reviewed elsewhere and in many books. 2,3 In this review, the other analytical applications of POMs are introduced, mainly focusing on papers published after 2000.…”

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confidence: 99%

Polyoxometalates in Analytical Sciences

Ueda

2020

ANAL. SCI.

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Polyoxometalates (POMs) have been used for spectrophotometric determinations of silicon and phosphorus under acidic conditions, referred to as the molybdenum yellow method and molybdenum blue method, respectively. Many POMs are redox active and exhibit fascinating but complicated voltammetric responses. These compounds can reversibly accommodate and release many electrons without exhibiting structural changes, implying that POMs can function as excellent mediators and can be applied to sensitive determination methods based on catalytic electrochemical reactions. In addition, some rare-earth-metal-incorporated POMs exhibit fluorescence, which enables sensitive determination by the enhancement and quenching of fluorescence intensities. In this review, various analytical applications of POMs are introduced, mainly focusing on papers published after 2000, except for the molybdenum yellow method and molybdenum blue method.

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