Determination of Pineal Melatonin by Precolumn Derivatization Reversed-Phase High-Performance Liquid Chromatography and Its Application to the Study of Circadian Rhythm in Rats and Mice

Hamase, Kenji; Tomita, Tatsunosuke; Kiyomizu, Ayako; Zaitsu, Kiyoshi

doi:10.1006/abio.1999.4435

Cited by 25 publications

(20 citation statements)

References 21 publications

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“…Concerning the mice, the amounts of pineal melatonin were reported to be trace in the ICR, C57BL, BALB/c mice (4.7, 6.1 and 7.4 fmol/pineal gland, respectively [23]). On the other hand, relatively high amounts of melatonin were observed in the pineal gland of the CBA and C3 H strains (54-550 fmol/ pineal gland [18,24,35]). The values of melatonin obtained in the present study were 1.24-3.56 fmol/pineal gland for the ICR, C57BL, BALB/c mice, and 349-372 fmol/pineal gland for the CBA and C3 H strains, which show no conflict with the already reported values.…”

Section: Determination Of Melatonin and The Relationship Between D-asmentioning

confidence: 94%

Enantioselective micro‐2D‐HPLC determination of aspartic acid in the pineal glands of rodents with various melatonin contents

Han

Miyoshi

Oyama

et al. 2011

J of Separation Science

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Enantioselective determination of aspartic acid (Asp) in the pineal gland of rodents with various melatonin contents was performed using a highly sensitive and selective two-dimensional HPLC system. After derivatization of the amino group with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), NBD-Asp was separated using a capillary monolithic ODS column in the first dimension. The fraction of NBD-Asp was automatically collected and transferred to the second dimension, and the D- and L-Asp were separated and determined using a narrowbore enantioselective column. Large amounts of D-Asp were observed in the pineal gland of the rats and specific strains of mice (C3H and CBA) possessing a high concentration of melatonin in their pineal gland. On the other hand, the amounts of D-Asp were small in the pineal gland of mice possessing a trace or no melatonin in their pineal gland (ddY, ICR, C57BL and BALB/c). In other tissues and physiological fluids, no significant strain-dependent changes of the D-Asp amounts were observed. These results indicate that large amounts of D-Asp are present only in the pineal gland containing large amounts of melatonin, and special care should be taken when selecting mouse strains for the investigation of D-Asp.

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Section: Determination Of Melatonin and The Relationship Between D-asmentioning

confidence: 94%

Enantioselective micro‐2D‐HPLC determination of aspartic acid in the pineal glands of rodents with various melatonin contents

Han

Miyoshi

Oyama

et al. 2011

J of Separation Science

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“…31 On the other hand, for the oxidation of melatonin in biological matrices, 200 mM Na2CO3 and 5 mM H2O2 were used with heating at 100 C for 30 min. [32][33][34] In the present investigation, the same reaction conditions could be used for both the standard melatonin and biological samples (10 mM Na2O3 and 0.2 mM H2O2, heated at 110 C for 30 min) due to the utilization of the on-line purification of melatonin. In all of the samples (standard solution and biological matrices), a sufficient reaction yield was obtained, which is a significant advantage of the present system.…”

Section: Design and Establishment Of An On-line Oxidation Unitmentioning

confidence: 99%

“…On the other hand, we have already reported a highly sensitive HPLC method for the determination of melatonin using precolumn oxidation and fluorescence detection. [31][32][33][34] By this method, melatonin is converted to an oxidized compound, N-[ (6-methoxy-4-oxo-1,4-dihydroquinolin-3yl)methyl] acetamide (6-MOQMA, Fig. 1), and the produced 6-MOQMA has a strong fluorescence emission at 382 nm with excitation at 246 nm.…”

Section: Introductionmentioning

confidence: 99%

Development of a Fully-automated On-line Oxidation Column-switching HPLC System for the Determination of Endogenous Melatonin in Human Clinical Samples

et al. 2015

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A fully-automated on-line oxidation column-switching HPLC system has been developed for the determination of endogenous melatonin in human plasma and saliva. This HPLC system consists of four processes. In the first step, melatonin is fractionated using a reversed-phase C4 column (Proteonavi, 75 mm × 1.0 mm i.d.). In the second step, the obtained melatonin fraction is on-line collected, and oxidized to a highly-fluorescent compound, N- [(6-methoxy-4-oxo-1,4-dihydroquinolin-3-yl)methyl]acetamide (6-MOQMA), by mixing with hydrogen peroxide under alkaline conditions. In the third step, the produced 6-MOQMA is concentrated, and the oxidation reagents are removed using an alkaline resistive reversed-phase column, Asahipak ODP (35 mm × 1.0 mm i.d.). In the final fourth step, the 6-MOQMA is determined by a microbore-ODS column packed with ultrafine particles (CAPCELL PAK C18 IF, 250 mm × 1.0 mm i.d.). The limit of detection of melatonin using this system is about 200 amol/injection, and the determination of endogenous melatonin in a small volume of human physiological fluids, such as 100 μL of plasma and 300 μL of saliva, was successfully accomplished.

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“…Recent studies have shown the beneficial effects of melatonin and the risk factor of the decreased quantities in old age. The different analytical methods that have been reported for its determination include HPLC [19][20][21][22][23][24][25], GC [26], capillary electrophoresis [27], micellar electrokinetic chromatography [28], spectrofluorimetry [29][30], voltammetric and flow amperometric [31,33].…”

Section: Introductionmentioning

confidence: 99%

Novel Potentiometric Sensors for Determination of Melatonin and Oxomemazine in Biological Samples and in Pharmaceutical Formulations

Saber

2010

Electroanalysis

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Simple, selective and accurate sensors were developed for the determination of melatonin and oxomemazine in biological samples (urine) and in pharmaceutical preparations. Potentiometric measurements were based on bismus tetraiodate-drug ion-pair as novel electroactive materials incorporating a plasticized PVC membrane with o-nitrophenyl octyl ether or dioctyl phthalate. Each sensor was conditioned for at least two days in 0.1 M drug solution before use. It exhibited fast and stable Nernstian response for melatonin and oxomemazine over the concentration range of 1.0 10 À6 -1.0 10 À2 M and 1.0 10 À5 -1.0 10 À2 M, pH range of 3.0-6.5 and 3.5-6.0 for melatonin and oxomemazine sensors, respectively. Results with an average recovery not more than 101 % and a mean standard deviation less than 1.0 % of the nominal were obtained for the four sensors. The sensors showed reasonable selectivity towards investigated drugs in presence of many cations.

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Determination of Pineal Melatonin by Precolumn Derivatization Reversed-Phase High-Performance Liquid Chromatography and Its Application to the Study of Circadian Rhythm in Rats and Mice

Cited by 25 publications

References 21 publications

Enantioselective micro‐2D‐HPLC determination of aspartic acid in the pineal glands of rodents with various melatonin contents

Enantioselective micro‐2D‐HPLC determination of aspartic acid in the pineal glands of rodents with various melatonin contents

Development of a Fully-automated On-line Oxidation Column-switching HPLC System for the Determination of Endogenous Melatonin in Human Clinical Samples

Novel Potentiometric Sensors for Determination of Melatonin and Oxomemazine in Biological Samples and in Pharmaceutical Formulations

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