Determination of sulfur-containing amino acids by capillary electrophoresis dynamic reaction cell inductively coupled plasma mass spectrometry

Yeh, Chingwei; Jiang, Shiuh‐Jen; Hsi, Tai-Sung

doi:10.1016/j.aca.2003.09.051

Cited by 50 publications

(43 citation statements)

References 32 publications

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“…Although the system proved to be robust, requiring minute sample volumes (only about 20 nL are injected into the capillary) and providing reproducible results with short analysis times (less than 10 min), implementation of reaction/collision cell technology for more accurate determination of the protein content would be favorable [46,47]. Complimentary CE-ESI-MS experiments would also help in the elucidation of the exact structure of the adducts, whereas application of capillary IEF for better separation of the proteins would surely aid in the identification of the drug's less abundant binding partners.…”

Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

et al. 2008

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Capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasmaThe development of metal-based chemotherapeutics lacks methods which are capable of providing early indication on the potential of new metal complexes as future anticancer drugs. Since most of these compounds are administered intravenously, serum proteins are the first available biological binding partners in the bloodstream. For platinum-based anticancer drugs the interaction with serum proteins is regarded as an important contribution to the side effects accompanying chemotherapy. In contrast, newly developed ruthenium compounds are thought to be transported into the tumor in a protein-bound form. In here, the application of CE hyphenated to inductively coupled plasma (ICP)-MS, applying Polybrene-coated capillaries, is demonstrated for studying the interaction of indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019) with HSA and transferrin, which are important transport proteins. Furthermore, the applicability of the method to human serum and plasma and, more importantly, to real-world patient samples was proven. KP1019 was found to bind to a high degree to HSA both in serum, plasma and the patient samples. Only minor fractions of ruthenium were found attached to other proteins.

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Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

et al. 2008

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“…Iodine disinfection by-products, in particular iodphenols, were determined in river water by Kannamkumarath et al [194]. Their method involves offline SPME followed by analysis of the extracts using CE coupled to ICP-MS. Sulfur containing amino acids were investigated employing CE-ICP-MS with a dynamic reaction cell by Yeh et al [195]. Analytes included in this study were cysteine, cystine, homocystine, and methionine, which were determined in urine and nutritive complement samples.…”

Section: Applications Of Ce-icp-msmentioning

confidence: 99%

Recent advances in the application of capillary electrophoresis with mass spectrometric detection

Klampfl

2006

Electrophoresis

116

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This review gives an overview of applications of CE coupled to MS detection published in the literature of the last three years. The works discussed in this paper comprise a wide range of different fields of application. These include important sections such as the analysis of biomolecules, the analysis of pharmaceuticals and their metabolites in different matrices, environmental analysis, and also investigations on the composition of technical products.

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“…A detailed description of the CEI-100 CE interface (CETAC, Omaha, NE, USA) was given in previous papers [17,21]. The nebulizer worked in the self-aspiration mode at a flow rate of around 5.5 mL/min [22]. A 0.1% v/v HNO 3 solution containing 5% methanol was used as the make-up solution to provide the electrical connection and to reduce surface …”

Section: Ce-icp-ms Systemmentioning

confidence: 99%

Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry

Yeh

Jiang

2005

Electrophoresis

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A capillary electrophoresis-inductively coupled plasma-mass spectrometric (CE-ICP-MS) method for the speciation of six arsenic compounds, namely arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine is described. The separation has been achieved on a 70 cm length x 75 microm ID fused-silica capillary. The electrophoretic buffer used was 15 mM Tris (pH 9.0) containing 15 mM sodium dodecyl sulfate (SDS), while the applied voltage was set at +22 kV. The arsenic species in biological tissues were extracted into 80% v/v methanol-water mixture, put in a closed centrifuge tube and kept in a water bath, using microwaves at 80 degrees C for 3 min. The extraction efficiencies of individual arsenic species added to the sample at 0.5 microg As/g level were between 96% and 107%, except for As(III), for which it was 89% and 77% for oyster and fish samples, respectively. The detection limits of the species studied were in the range 0.3-0.5 ng As/mL. The procedure has been applied for the speciation analysis of two reference materials, namely dogfish muscle tissue (NRCC DORM-2) and oyster tissue (NIST SRM 1566a), and two real-world samples.

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Determination of sulfur-containing amino acids by capillary electrophoresis dynamic reaction cell inductively coupled plasma mass spectrometry

Cited by 50 publications

References 32 publications

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

Recent advances in the application of capillary electrophoresis with mass spectrometric detection

Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry

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