Determination of the Antibacterial Effects of Prunus amygdalinus (Almond Leaf) and Ocimum Gratissimum (Basil Leaf) on Selected Bacteria

Abstract

The antimicrobial acts of leaf extracts of Ocimumgratissimum and Prunusamygdalinus were checked on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi using agar cup plate method. The outcomes demonstrated that the chosen organisms were susceptible to the plant extracts at concentrations of 500mg, 50mg and 5mg respectively with diameter zone of inhibition (DZI) of about 12.00± 2.00, 13.00± 2.00, 11.60±3.40, and 12.30±3.30. Phytochemical screening of the leaf extract of Ocimumgratissimum manifested the existence of alkaloid, phenols, glycosides, saponin, steroids and triterpenes while the leaf extract of Prunusamygadalinus demonstrated the existence of saponin, flavonoid and triterpenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assayed reveals MIC of 5mg and MBC of 50mg respectively. Ocimumgratissimum and Prunusamygdalinus extracts may be applied to treat disorder caused by these organisms.