Determination of the elemental composition of unknown aroma constituents by using a quadrupole mass spectrometer

Model reactions on roast aroma formation. 14. Formation of 2-acetylpyrido[3,4-d]imidazole by heating of glucose with histidine

“…The same peak was also obtained when we reacted histamine with pyruvic aldehyde under the same conditions. Another indication of the structure of III was obtained by exact mass determination (Wittkowski et al, 1984), resulting in the molecular formula C8H7N3O. By treatment of the compound with CD3OD, two to three hydrogen atoms were exchanged, because the acetyl group can possess active hydrogen atoms by means of keto-enol tautomerism.…”