Determination of the epimerization rate constant of amygdalin by microemulsion electrokinetic chromatography

Abstract

A new method for separation and determination of amygdalin and its epimer (neoamygdalin) in the epimerization of amygdalin by MEEKC is proposed. For the chiral separation of amygdalin and neoamygdalin, a running buffer composed of 80 mM sodium cholate, 5.0% v/v butan-1-ol, 0.5% v/v heptane and 94.5% v/v 30 mM Na(2) B(4) O(7) buffer (pH 9.00) is proposed. Under optimum conditions, the basic separation of amygdalin and neoamygdalin can be achieved within 7 min. The calibration curve for amygdalin showed excellent linearity in the concentration range of 20-1000 μg/mL with a detection limit of 5.0 μg/mL (S/N=3). The epimerization rate constant of amygdalin in basic microemulsion was first determined by monitoring the concentration changes of amygdalin, and the epimerization rate constant of amygdalin was found to be 2×10(-3) min(-1) at 25°C under the above optimum microemulsion conditions.

Amygdalin isolated from Semen Persicae (Tao Ren) extracts induces the expression of follistatin in HepG2 and C2C12 cell lines

“…Amygdalin was identified from the S. Persicae extract as an inducer of follistatin. Amygdalin existed in two forms, D-amygdalin and neoamygdalin [28,29]. The two closely eluted peaks in the HPLC profiles of fraction F5 and the amygdalin standard were observed, which might reflect the two forms of amygdalin.…”

Chiral electromigration techniques in pharmaceutical and biomedical analysis

“…Rate constants of the individual reactions were determined. Furthermore, the racemization kinetics of Tic-hydantoin derivatives has been investigated by chiral CE and HPLC [97] and the epimerization of the glycoside amygdaline was studied employing a MEEKC method [98]. Chiral CE was further employed to study the in vitro formation of the neurotoxin N-methylsalsolinol, a tetrahydroisoquinoline derivative, by reaction of the catecholamine epinine with acetaldehyde [99].…”

A review of developments in the methodology and application of microemulsion electrokinetic chromatography

“…In addition to sample concentration, chiral analysis, capillary coating, and characterization, a number of novel MEEKC ME additives and applications have been developed in the past two years. These have included the use of carbon nanotubes (CNTs) , ionic liquid (IL)‐in‐water MEEKC , and epimerization monitoring . Continuing advances have also been made in the area of multiplexed MEEKC .…”

“…Yu et al developed an MEEKC method for the separation of the glycoside amygdalin and its epimer neoamygdalin. The method was employed to estimate the rate of epimerization.…”