Determination of the immunotoxic potential of pesticides on functional activity of sheep leukocytes in vitro

Pistl, J.; Kovalkovičová, Natália; Holovská, Vanda; Legáth, Jaroslav; Mikula, I.

doi:10.1016/s0300-483x(03)00046-5

Cited by 34 publications

(18 citation statements)

References 12 publications

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“…After leukocyte isolation from the peripheral blood [14], we analyzed the index of metabolic activity of phagocytes (IMA) by iodine-nitro-tetrasolium reductase test [15]; stimulation index of lymphocytes (SI) by commercial test kits (Cell Proliferation ELISA Kit, BrdU-colorimetric, Roche) with ELISA reader [16]; and total immunoglobulins (TIg) by turbidimetric spectrophotometric zinc-sulphate test [17].…”

Section: Estimation Of Phagocyte Activity and Total Immunoglobulinsmentioning

confidence: 99%

Influence of beta-glucan and vaccination against Lawsonia intracellularis on selected immune indices in weaned piglets

Kovačocyová¹,

Reichel²,

Seidel³

et al. 2014

Acta Veterinaria

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The aim of this paper was to study the immunostimulating effects of feed additive containing beta-glucan given to pregnant sows and consecutive specific immunomodulation by vaccination of their suckling piglets. Thirty-five suckling piglets from the control and experimental group of sows were divided into four groups. Selected blood indices of immune profile (TIg, IMA, SI), specific antibodies against Lawsonia intracellularis formed in the blood serum of suckling piglets after vaccination, as well as production indices were observed. In all the groups, birth body weights were similar. After weaning, average daily weight gains were significantly higher (p < 0.05) in the group of vaccinated suckling piglets, as well as in suckling piglets from experimental sows. The same trend was recorded in average TIg values. Significant effects (p < 0.01) of beta-glucan and vaccination were observed in the changes of the index of metabolic activity of phagocytes (IMA) and less markedly in the index of stimulation activity of lymphocytes (SI). The least favorable results were observed in the group without both vaccination and beta-glucan. The effect of vaccination was manifested by ELISA estimation of specific antibodies (PI), when in both non-vaccinated groups (A and D) we observed minimum antibodies levels compared with vaccinated groups (B and C), which showed a high positivity without significant differences between the beta-glucan stimulated (C) and the non-stimulated group (B). These results suggest that beta-glucan given to sows and consecutive vaccination of suckling piglets can stimulate their non specific and specific immunity.

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Section: Estimation Of Phagocyte Activity and Total Immunoglobulinsmentioning

confidence: 99%

Influence of beta-glucan and vaccination against Lawsonia intracellularis on selected immune indices in weaned piglets

Kovačocyová¹,

Reichel²,

Seidel³

et al. 2014

Acta Veterinaria

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“…Test was made according to applied guide, and 10 000 leukocytes were counted for interpretation. Stimulation index of leukocytes (SI) was assessed by commercial test (Cell Proliferation ELISA Kit, BrdU-colorimetric, Roche) and measured by ELISA reader (Pistl et al 2003). Blood serum total immunoglobulines (TIg) were analysed spectrophotometrically by turbidimetric method with zinc-sulphate test (Slanina et al 1976).…”

Section: Sample Analysismentioning

confidence: 99%

Beta-glucan feeding effect on biochemical and immune responses in vaccinated and non-vaccinated piglets against proliferative enteropathy

et al. 2013

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The aim of this study was to compare the results of active non-specific immuno-modulation in form of feeding food additive based on beta-glucan to pregnant sows and consecutive specific immuno-modulation by vaccination of their sucklings. Experimental sows were fed feedstuff with preparation on basis of beta-glucan at a 5% concentration from day 14 before parturition until the weaning of piglets; control sows were fed standard feedstuff only. Sucklings were vaccinated with a single dose of 2 ml of oral vaccine Enterisol ® Ileitis one week before weaning. Collection of biological material was done 3 × in sows and 5 × in sucklings. Postvaccination examination of blood serum for antibodies against L. intracellularis in sucklings in experimental and control groups was negative, probably due to insufficiently long period of rejection of antibiotics-medicated feedstuff and by chlorinated water. With the exception of immunological profile, no essential changes were recorded in the dynamics of other indices of examined profiles in groups of sows in relation to immune-modulation. In groups of sucklings from sows fed beta-glucan supplemented feedstuff significant changes were determined for various indices compared with sucklings from sows fed standard foodstuff. This is the first similar study in pig herds in Slovakia.

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“…Moreover, the increment in plasma urea and creatinine levels and the reduction of uric acid in plasma along with histopathological alterations of 2,4-D-treated rats reported by Tayeb et al [8] also supported the impairment of renal functions. Teratogenic [11][12][13], genotoxic [14], neurotoxic [2,[15][16][17], immunosuppressive [18], cytotoxic [19][20], and hepatotoxic [21][22][23] effects of 2,4-D have also been well documented.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Melatonin Against 2,4-Dichlorophenoxyacetic Acid Toxicity in Kidney of Mice – A Histological Study.

Upadhyaya

Rao

Jhala

2018

Asian J Pharm Clin Res

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Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II -melatonin alone (10 mg/kg body weight); Group III -2,4-D alone (50 mg/kg body weight); and Group IV -2,4-D + melatonin was given orally for 45 days.Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules. Conclusion:Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity.

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Determination of the immunotoxic potential of pesticides on functional activity of sheep leukocytes in vitro

Cited by 34 publications

References 12 publications

Influence of beta-glucan and vaccination against Lawsonia intracellularis on selected immune indices in weaned piglets

Influence of beta-glucan and vaccination against Lawsonia intracellularis on selected immune indices in weaned piglets

Beta-glucan feeding effect on biochemical and immune responses in vaccinated and non-vaccinated piglets against proliferative enteropathy

Ameliorative Effects of Melatonin Against 2,4-Dichlorophenoxyacetic Acid Toxicity in Kidney of Mice – A Histological Study.

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