Determination of the Processing Sites of an <i>Arabidopsis</i> 2S Albumin and Characterization of the Complete Gene Family

Krebbers, Enno; Herdies, Lydia; Clercq, Adelbert De; Seurinck, Jef; Leemans, J.; Damme, Jozef Van; Arnal, Magdalena; Gheysen, Godelieve; Montagu, Marc Van; Vandekerckhove, Joël

doi:10.1104/pp.87.4.859

Cited by 193 publications

(150 citation statements)

References 26 publications

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“…In oilseed rape [100], castor bean [101], Arabidopsis [102], brazil nut [103] and lupin [104] these have similar structures, the mature proteins consisting of two subunits associated by interchain disulphide bond(s). The small subunits at about 25-40 residues contain region A, and the large Fig.…”

Section: S Storage Proteinsmentioning

confidence: 99%

The prolamin storage proteins of cereal seeds: structure and evolution

Shewry

Tatham

1990

Biochemical Journal

639

421

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Section: S Storage Proteinsmentioning

confidence: 99%

The prolamin storage proteins of cereal seeds: structure and evolution

Shewry

Tatham

1990

Biochemical Journal

639

421

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“…For protein gel blotting, we used antibodies for two members of PSI complexes (PsaA and PsaB), three members of PSII complexes (PsbB, PsbD, and OEC33;Jain et al, 1998;Jeong et al, 2003), the photosynthetic and respiratory ATP synthase (AtpB; Inatomi, 1986), RbcS (Krebbers et al, 1988), and Lhcb3 (Jackowski et al, 2001). Our data showed that PsaB, PsbD, RbcS, and Lhcb3 proteins were undetectable in the ptac14-1 mutant, while the levels of PsaA, PsbB, and OEC33 proteins were significantly decreased in the ptac14-1 mutant compared with the wild-type plant, and the accumulation of AtpB was less affected in ptac14-1.…”

Section: Chloroplast Development Is Defective In Ptac14 Mutantsmentioning

confidence: 99%

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

et al. 2011

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The SET domain-containing protein, pTAC14, was previously identified as a component of the transcriptionally active chromosome (TAC) complexes. Here, we investigated the function of pTAC14 in the regulation of plastid-encoded bacterialtype RNA polymerase (PEP) activity and chloroplast development. The knockout of pTAC14 led to the blockage of thylakoid formation in Arabidopsis (Arabidopsis thaliana), and ptac14 was seedling lethal. Sequence and transcriptional analysis showed that pTAC14 encodes a specific protein in plants that is located in the chloroplast associated with the thylakoid and that its expression depends on light. In addition, the transcript levels of all investigated PEP-dependent genes were clearly reduced in the ptac14-1 mutants, while the accumulation of nucleus-encoded phage-type RNA polymerase-dependent transcripts was increased, indicating an important role of pTAC14 in maintaining PEP activity. pTAC14 was found to interact with pTAC12/ HEMERA, another component of TACs that is involved in phytochrome signaling. The data suggest that pTAC14 is essential for proper chloroplast development, most likely by affecting PEP activity and regulating PEP-dependent plastid gene transcription in Arabidopsis together with pTAC12.

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“…Genomic libraries were constructed from the RUG-AT2 and RUG-ATIO lines as described by Krebbers et al (1988). The RUG-AT2 genomic library, made from a pool of hemizygous and homozygous T2 plants, was used to clone all target plant DNA sequences described in this work.…”

Section: Construction and Screening Of Phage A Genomic Librariesmentioning

confidence: 99%

Illegitimate recombination in plants: a model for T-DNA integration.

Gheysen¹,

Villarroel²,

Montagu³

1991

Genes Dev.

266

201

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Agrobacterium tumefaciens is a soil bacterium capable of transferring DNA (the T-DNA) to the genome of higher plants, where it is then stably integrated. Six T-DNA inserts and their corresponding preinsertion sites were cloned from Arabidopsis thaliana and analyzed. Two T-DNA integration events from Nicotiana tabacum were included in the analysis. Nucleotide sequence comparison of plant target sites before and after T-DNA integration showed that the T-DNA usually causes only a small (13-28 bp) deletion in the plant DNA, but larger target rearrangements can occur. Short homologies between the T-DNA ends and the target sites, as well as the presence of filler sequences at the junctions, indicate that T-DNA integration is mediated by illegitimate recombination and that these processes in plants are very analogous to events in mammalian cells. We propose a model for T-DNA integration on the basis of limited base-pairing for initial synapsis, followed by DNA repair at the junctions. Variations of the model can explain the formation of filler DNA at the junctions by polymerase slipping and template switching during DNA repair synthesis and the presence of larger plant target DNA rearrangements.

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Determination of the Processing Sites of an Arabidopsis 2S Albumin and Characterization of the Complete Gene Family

Cited by 193 publications

References 26 publications

The prolamin storage proteins of cereal seeds: structure and evolution

The prolamin storage proteins of cereal seeds: structure and evolution

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

Illegitimate recombination in plants: a model for T-DNA integration.

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