Determination of thirteen acidic phytohormones and their analogues in tea (Camellia sinensis) leaves using ultra high performance liquid chromatography tandem mass spectrometry

Jiang, Changling; Dai, Jingjie; Han, Haolei; Wang, Chen; Zhu, Li; Lu, Chengyin; Chen, Hongping

doi:10.1016/j.jchromb.2020.122144

Cited by 23 publications

(13 citation statements)

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“…In the step of concentrating phytohormones, chemists prefer nitrogen blowing rather than rotary evaporation [44,52,54,63]. Carolina et al compared these two methods, and observed that although rotary evaporation took a shorter time, the loss of the targeted hormones was twice that of nitrogen blowing [38].…”

Section: Enrichment Processmentioning

confidence: 99%

“…Chromatographic methods, including gas chromatography (GC) and liquid chromatography (LC) connected in series with different types of mass spectrometry (MS), have excellent sensitivity and separation ability, and meet the requirements for simultaneous detection of multiple hormones in complex matrices [39][40][41][42][43]. Compared with GC-MS, LC-MS has the advantage of facilitating the analysis of thermally labile compounds and does not require derivatization, so it has become the most important method for detecting phytohormones [38,44]. In recent years, ultra-high-performance liquid chromatography combined with triple quadrupole mass spectrometry (UHPLC-QqQ-MS) has achieved great success in the fields of pharmaceutical, biochemical, food and environmental analysis.…”

Section: Introductionmentioning

confidence: 99%

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A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

et al. 2022

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Phytohormones play an important role in regulating the maturation process and the quality-related metabolite accumulation of fruits, and their concentration variation has always been concerned during fruit development. However, berry fruits, such as grape berries, are rich in a large number of secondary metabolites, which brings great challenges to the isolation and determination of hormones. In this work, we used grapes as experimental materials and proposed a solid-phase extraction (SPE) protocol to efficiently isolate multiple hormones from phenol-rich matrix using a mixture of dichloromethane, methanol and formic acid as eluent. A highly sensitive method based on ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) was developed to quantify a total of 11 plant growth regulators, including the recognized phytohormones, in grape pericarp and seed. The established method showed satisfactory precision (RSD < 11.3%) and linearity (R2 > 0.9980). The limits of detection (LOD) and the limit of quantification (LOQ) were 0.001–0.75 ng/mL and 0.004–2.5 ng/mL, respectively. The recovery for the three levels of analytes spiked ranged from 63% to 118%, and the matrix effect was between 73% and 119%. Finally, the proposed method was applied to investigate the dynamic hormone concentration in Vitis vinifera L. cv. Cabernet Sauvignon berries from different vineyards, and assess the changes in endogenous hormones in grapes after treatment with exogenous growth regulators. We found that the contents of IP, ABA and IAA in pericarp and IP, IAA, IBA and SA in seed were significantly down-regulated after 10 days of treatment with NAA concentrations of 10 mg/L and 40 mg/L. In conclusion, this method helps to elucidate the role played by phytohormones in the maturation process and the accumulation of quality-related metabolites in phenol-rich fruits.

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Section: Enrichment Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

et al. 2022

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“…Endogenous levels of ABA, GA 3 , IAA, and TZR were determined as described by [ 36 ]. Freshly harvested seeds (100 mg) at 73, 94, 115, 129, and 150 DAP were ground in liquid nitrogen, and the extraction was performed with acetonitrile solution.…”

Section: Methodsmentioning

confidence: 99%

Abscisic Acid Inhibits Asymbiotic Germination of Immature Seeds of Paphiopedilum armeniacum

Fang

et al. 2020

IJMS

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Paphiopedilum armeniacum is a rare orchid native to China with high ornamental value. The germination of P. armeniacum seeds is difficult, especially for the mature seeds, which is the major limitation for their large-scale reproduction. This study explored the reasons for seed germination inhibition from the aspects of the important plant endogenous hormone—abscisic acid (ABA). The major endogenous hormone contents of seeds were determined at different developmental stages. The ABA content was 5.8 ng/g in 73 days after pollination (DAP) for the immature seeds, peaked at 14.6 ng/g in 129 DAP seeds, and dropped to 2.6 ng/g in the late mature stage of the 150 DAP seeds. The reduction of ABA content in the mature seed suggests a possible contribution to the increased expression of CYP707A, an ABA catabolism gene. The germination rate of the immature seeds was reduced to 9% from 69% when 5 μg/mL ABA was added to the Hyponex N026 germination medium. The result showed that ABA can inhibit the germination of P. armeniacum immature seeds. However, for the heavily lignified mature seeds, reduction in endogenous ABA level does not result in an increase in the germination rate. Lignin accumulation in the seed coat imposes the physical dormancy for P. armeniacum. In summary, the germination of P. armeniacum is regulated by both ABA and lignin accumulation.

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“…The interaction of plant hormones is important for normal plant development, and in recent years, there has been more attention in the accurate quantification of plant hormones. Research studies range in size from only one plant hormone [9,10] to the simultaneous investigation of many plant hormones [11][12][13]. However, plant hormones are difficult to accurately analyse because of complex matrix interference, as well as low hormone concentrations (ng g À1 fresh weight) [14].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, sample concentration and purification techniques are necessary prior to chromatographic analysis. To date, there have been a variety of enrichment and purification methods for preparing plant hormone samples, such as immunoaffinity purification [20], liquid-liquid extraction [21], solid-phase microextraction [22], solidphase extraction [11], micro-solid-phase extraction [18], and magnetic solid-phase extraction (MSPE) [23]. MSPE is a convenient solid-phase extraction method based on magnetic or magnetisable adsorbents that exhibits many advantages in the field of separation science [24].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of magnetic ordered mesoporous carbon for quantitative analysis of acidic phytohormones in mushroom samples prior to their determination by ultra-high-performance liquid chromatography–tandem mass spectrometry

Zhang

Chen

Yang

et al. 2023

AChrom

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A novel method was established for analysing trace four acidic phytohormones, namely, indole-3-acetic acid, 3-indolebutyric acid, abscisic acid, and 1-naphthylacetic acid, using magnetic ordered mesoporous carbon (MOMC). MOMC was facilely synthesised via self-assembly strategy with a direct carbonisation process. The properties of MOMC were characterised using various instruments. MOMC exhibited excellent adsorption capacity towards the analytes. Various critical parameters which may influence the enrichment efﬁciency were evaluated, including amount of MOMC, extraction conditions, and desorption conditions. An efficient method based on MOMC magnetic solid-phase extraction coupled with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) was developed to analyse the trace four acidic phytohormones, with good correlation coefﬁcients (R 2 = 0.9965–0.9998) and low limits of detection (0.13–9.7 ng L−1, S/N = 3). Trace acidic phytohormones in Agaricus bisporus and Hypsizygus marmoreus samples were determined with satisfactory recoveries (91.8–108%) and reproducibility (2.6–6.3%). The features indicated that MOMC provides an efficient platform for mushroom sampling; the developed method is convenient, promising, and sensitive for the detection of trace phytohormones in complicated mushroom samples.

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Determination of thirteen acidic phytohormones and their analogues in tea (Camellia sinensis) leaves using ultra high performance liquid chromatography tandem mass spectrometry

Cited by 23 publications

References 50 publications

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

Abscisic Acid Inhibits Asymbiotic Germination of Immature Seeds of Paphiopedilum armeniacum

Fabrication of magnetic ordered mesoporous carbon for quantitative analysis of acidic phytohormones in mushroom samples prior to their determination by ultra-high-performance liquid chromatography–tandem mass spectrometry

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