Determination of trace aflatoxin M1 (AFM1) residue in milk by an immunochromatographic assay based on (PEI/PSS)4 red silica nanoparticles

Su, Zixian; Zhao, Guangying; Dou, Wenchao

doi:10.1007/s00604-020-04636-6

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…High-performance liquid chromatography (HPLC) can be coupled with ultraviolet (UV) or fluorescence (FL) detectors. These methods are not optimal for screening several samples compared with immunological-based methods, such as enzyme-linked immunosorbent assay (ELISA) [ 21 , 22 , 23 ], immunochromatographic assay (ICA) [ 24 , 25 , 26 ], or lateral flow immunoassay (LFIA) [ 27 , 28 ]. The need to analyse several samples, providing fast and reliable results, as well as the need to use the lowest possible volume of sample, make ELISA the best method.…”

Section: Introductionmentioning

confidence: 99%

Compliance between Food and Feed Safety: Eight-Year Survey (2013–2021) of Aflatoxin M1 in Raw Milk and Aflatoxin B1 in Feed in Northern Italy

Ferrari

Rizzi

Grandi

et al. 2023

Toxins

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Aflatoxins (AFs) are fungal metabolites that are found in feed and food. When ruminants eat feed contaminated with aflatoxin B1 (AFB1), it is metabolised and aflatoxin M1 (AFM1) is excreted in the milk. Aflatoxins can result in hepatotoxic, carcinogenic, and immunosuppressive effects. The European Union thus set a low threshold limit (50 ng/L) for presence of AFM1 in milk. This was in view of its possible presence also in dairy products and that quantification of these toxins is mandatory for milk suppliers. In the present study, a total of 95,882 samples of whole raw milk, collected in northern Italy between 2013 and 2021, were evaluated for presence of AFM1 using an ELISA (enzyme-linked immunosorbent assay) method. The study also evaluated the relationship between feed materials collected from the same farms in the same area during the same period (2013–2021) and milk contamination. Only 667 milk samples out of 95,882 samples analysed (0.7%) showed AFM1 values higher than the EU threshold limit of 50 ng/L. A total of 390 samples (0.4%) showed values between 40 and 50 ng/L, thus requiring corrective action despite not surpassing the regulatory threshold. Combining feed contamination and milk contamination data, some feedingstuffs seem to be more effective in defying potential carryover of AFs from feed to milk. Combining the results, it can be concluded that a robust monitoring system that covers both feed, with a special focus on high risk/sentinel matrices, and milk is essential to guarantee high quality and safety standards of dairy products.

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Section: Introductionmentioning

confidence: 99%

Compliance between Food and Feed Safety: Eight-Year Survey (2013–2021) of Aflatoxin M1 in Raw Milk and Aflatoxin B1 in Feed in Northern Italy

Ferrari

Rizzi

Grandi

et al. 2023

Toxins

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“…Due to their chemical reactivity, many organic dyes are very suitable for dyeing, such as dyeing colorless SiNPs into colored SiNPs. Besides, the surface of SiNPs is easy to modify, which can be used in catalysis, molecular detection, biological detection, and imaging by doping functional materials ( 12 , 13 ). Therefore, colored SiNPs not only possess a bright color but also possess four advanced characteristics: a strong covalent interaction between dye and SiNPs, high hydrophilicity and good water dispersion, excellent stability, and easy surface modification ( 5 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Wang

Lian

et al. 2021

Front. Vet. Sci.

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Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious experimental procedure and a sophisticated experimental device and performance. To overcome these defects, it is truly necessary to establish a real-time, on-site, and rapid detection method for human brucellosis. Here, a lateral flow immunoassay (LFIA) with a rapid, sensitive, and alternative diagnostic procedure for human brucellosis with a high degree of accuracy was developed based on blue silica nanoparticles (SiNPs), Staphylococcal protein A (SPA), and surface Lipopolysaccharide of Brucella spp. (LPS), which can be applied for rapid and feasible detection of human brucellosis. To our knowledge, this is the first report that uses blue SiNPs as a signal probe of LFIA for the rapid diagnosis of human brucellosis. The precursor of blue SiNPs@SPA such as colorless SiNPs and blue SiNPs was synthesized at first and then coupled with SPA onto the surface of blue SiNPs by covalent bond to prepare blue SiNPs@SPA as a capture signal to catch the antibody in the brucellosis-positive serum. When SPA was combined with the antibodies in the brucellosis-positive serum, it was captured by LPS on the test line, forming an antigen–antibody sandwich structure, resulting in the T line turning blue. Finally, the results showed that it is acceptable to use blue SiNPs as visible labels of LFIA, and standard brucellosis serum (containing Brucella spp. antibody at 1,000 IU/ml) could be detected at a dilution of 10−5 and the detection limit of this method was 0.01 IU/ml. Moreover, it also demonstrated good specificity and accuracy for the detection of real human serum samples. Above all, the blue SiNPs-based LFIA that we developed provides a rapid, highly accurate, and inexpensive on-site diagnosis of human brucellosis, and shows great promise in clinical diagnostics for other diseases.

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“…A excreção da AFM 1 se manifesta por meio da urina, leite, bile e fezes em um intervalo de 24 horas após a exposição (Alshannaq & Yu, 2017;Corassin et al, 2013;Memiş & Yalçın, 2021). Importante ressaltar que a velocidade de eliminação varia entre indivíduos, estágios de lactação e número de ordenhas realizadas após a exposição do animal às aflatoxinas (Su et al, 2020).…”

Section: Aflatoxinasunclassified

Avaliação da eficácia de diferentes adsorventes para redução de efeitos tóxicos combinados de micotoxinas na alimentação de vacas em lactação

Fonsêca

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Avaliação da eficácia de diferentes adsorventes para redução de efeitos tóxicos combinados de micotoxinas na alimentação de vacas em lactação / Luzianna Macedo Fonseca. --versão revisada de acordo com a Resolução CoPGr 6018 de 2011 --Piracicaba, 2024. 90 p. Dissertação (Mestrado) --USP / Escola Superior de Agricultura "Luiz de Queiroz".1. Multi-micotoxinas 2. Aditivos antimicotoxinas 3. Redução de micotoxinas 4. Vacas leiteiras 5. Adsorventes I. Título AGRADECIMENTOSPrimeiramente, agradeço a Deus, por Sua constante orientação e força que me permitiram superar desafios e me tornar uma pessoa melhor, valorizando ainda mais a vida. Agradeço por me ajudar a crescer diariamente.A mim por nunca desistir, por enfrentar noites mal dormidas, medos e ansiedades. Esses desafios foram fundamentais na construção das pontes que me levaram ao orgulho de superá-los e alcançar com êxito meus objetivos.À minha avó, Maria Edite Venceslau Fonseca (in memoriam), agradeço por ter me apoiado incondicionalmente em momentos difíceis ao longo da minha jornada acadêmica e de vida. Ela esteve sempre ao meu lado.

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Determination of trace aflatoxin M1 (AFM1) residue in milk by an immunochromatographic assay based on (PEI/PSS)4 red silica nanoparticles

Cited by 9 publications

References 40 publications

Compliance between Food and Feed Safety: Eight-Year Survey (2013–2021) of Aflatoxin M1 in Raw Milk and Aflatoxin B1 in Feed in Northern Italy

Compliance between Food and Feed Safety: Eight-Year Survey (2013–2021) of Aflatoxin M1 in Raw Milk and Aflatoxin B1 in Feed in Northern Italy

Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles

Avaliação da eficácia de diferentes adsorventes para redução de efeitos tóxicos combinados de micotoxinas na alimentação de vacas em lactação

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