Determination of trace elements in rat organs implanted with endodontic repair materials by ICP-MS

Abstract

To investigate the levels of seven elements using an inductively coupled plasma-mass spectrometry (ICP-MS) method in rat organs after the implantation of Micro Mega Mineral Trioxide Aggregate (MM-MTA), Bioaggregate (BA) and Biodentine (BD) materials. MM-MTA, BA and BD were implanted into the subcutaneous tissue of 15 Wistar albino rats; three control animals had no operation. After 45 days, the rats were sacrificed and their brains, kidneys and livers were removed. The ICP-MS analysis was used to determine trace elements. Data were analysed using the Kruskal-Wallis and Connover post hoc tests. There was no significant difference between the control groups and the MM-MTA, BA and BD groups according to the concentration of aluminum, calcium, arsenic and lead in the rats' organs. Beryllium was not detected in all tissue samples. Chromium levels of these materials were higher than the control group in brain and kidney samples (P = 0.038 and P = 0.037); magnesium levels were higher than the control group in kidney and liver samples (P = 0.030 and P = 0.008). MM-MTA, BA and BD were nontoxic according to trace element levels in brain, kidney and liver samples of rats. Further investigation is required to understand the systemic effects of these materials.

Systemic bone marker expression induced by grey and white mineral trioxide aggregate in normal and diabetic conditions

“…In addition, difference in the biocompatibility between both MTA materials on day 7 (Shahi et al 2006) could be associated with the higher concentration of metallic oxide in GMTA (Asgary et al 2006), which interferes with ionic dissociation. Investigations also revealed that the level of trace metal ions from MTA increased in the organs, such as liver, in rats (Demirkaya et al 2016, Simsek et al 2016. Investigations also revealed that the level of trace metal ions from MTA increased in the organs, such as liver, in rats (Demirkaya et al 2016, Simsek et al 2016.…”

“…In the present study, the serum calcium levels of both the MTA groups were higher than that of the control group on day 7, while it decreased on day 30. Traces of Ca 2+ released from the MTA have been detected in organs such as the liver, kidney and brain (Simsek et al 2016). The results revealed higher Ca 2+ release during the initial period, followed by subsequent reduction with time (De Vasconcelos et al 2009).…”

“…Therefore, diabetes mellitus is a chronic metabolic inflammatory disease associated with altered calcium homoeostasis and bone marker expression (Levy et al 1994). While the systemic effects of MTA treatment are already documented (Khalil & Eid 2013, Demirkaya et al 2016, Simsek et al 2016, no study has addressed its systemic effects on the expression of bone markers under diabetic conditions. While the systemic effects of MTA treatment are already documented (Khalil & Eid 2013, Demirkaya et al 2016, Simsek et al 2016, no study has addressed its systemic effects on the expression of bone markers under diabetic conditions.…”

“…The aim of this study was to investigate the relationship between diabetes mellitus and the systemic and local effects of GMTA and WMTA treatment on the expression of bone markers. While the systemic effects of MTA treatment are already documented (Khalil & Eid 2013, Demirkaya et al 2016, Simsek et al 2016, no study has addressed its systemic effects on the expression of bone markers under diabetic conditions. The ability of MTA to release Ca +2 upon hydration has been reported previously (De Vasconcelos et al 2009).…”

“…Demirkaya et al (2016) reported that the substances released by MTA in surrounding tissues can enter the bloodstream and can be transported to distant sites upon tissue damage. Traces of Ca 2+ released from the MTA have been detected in organs such as the liver, kidney and brain (Simsek et al 2016). Calcium homoeostasis is controlled by the influx of ions across the electrical and chemical gradients through calcium channels, as the physical mechanisms for solute selectivity favour the transport of ions through the interstitial fluid in blood (Rizzoli & Bonjour 2006, Guyton & Hall 2011.…”