Determination of urinary bromophenols (BrPs) as potential biomarkers for human exposure to polybrominated diphenyl ethers (PBDEs) using gas chromatography-tandem mass spectrometry (GC–MS/MS)

Feng, Chao; Xu, Qian; Jin, Young-Woo; Lin, Yuanjie; Qiu, Xinlei; Lu, Dasheng; Wang, Guoquan

doi:10.1016/j.jchromb.2016.03.041

Cited by 33 publications

(14 citation statements)

References 12 publications

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“…Another uVOM identified in this work, 2-bromophenol (X184, Figure 5), has been previously reported as a putative urinary volatile biomarker for BC [29]. This is a metabolite of polybrominated diphenyl ethers (PBDEs) that became widespread in the environment due to the massive use of wood preservatives and fire retardants [49], but it is also a primary metabolite essential for cell growth [43]. Tricyclazole (X190 in Figure 5) is most probably an environmental contaminant.…”

Section: Label Amentioning

confidence: 67%

Urinary Volatomic Expression Pattern: Paving the Way for Identification of Potential Candidate Biosignatures for Lung Cancer

et al. 2022

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The urinary volatomic profiling of Indian cohorts composed of 28 lung cancer (LC) patients and 27 healthy subjects (control group, CTRL) was established using headspace solid phase microextraction technique combined with gas chromatography mass spectrometry methodology as a powerful approach to identify urinary volatile organic metabolites (uVOMs) to discriminate among LC patients from CTRL. Overall, 147 VOMs of several chemistries were identified in the intervention groups—including naphthalene derivatives, phenols, and organosulphurs—augmented in the LC group. In contrast, benzene and terpenic derivatives were found to be more prevalent in the CTRL group. The volatomic data obtained were processed using advanced statistical analysis, namely partial least square discriminative analysis (PLS-DA), support vector machine (SVM), random forest (RF), and multilayer perceptron (MLP) methods. This resulted in the identification of nine uVOMs with a higher potential to discriminate LC patients from CTRL subjects. These were furan, o-cymene, furfural, linalool oxide, viridiflorene, 2-bromo-phenol, tricyclazole, 4-methyl-phenol, and 1-(4-hydroxy-3,5-di-tert-butylphenyl)-2-methyl-3-morpholinopropan-1-one. The metabolic pathway analysis of the data obtained identified several altered biochemical pathways in LC mainly affecting glycolysis/gluconeogenesis, pyruvate metabolism, and fatty acid biosynthesis. Moreover, acetate and octanoic, decanoic, and dodecanoic fatty acids were identified as the key metabolites responsible for such deregulation. Furthermore, studies involving larger cohorts of LC patients would allow us to consolidate the data obtained and challenge the potential of the uVOMs as candidate biomarkers for LC.

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Section: Label Amentioning

confidence: 67%

Urinary Volatomic Expression Pattern: Paving the Way for Identification of Potential Candidate Biosignatures for Lung Cancer

et al. 2022

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“…In other studies, Feng et al. ( 17 ) detected 2,4,6-TBP in the concentrations of 5.57 ± 4.05 μg/L in the urine of the general population of China, while Gutierrez et al. ( 18 ) found very high mean concentration of 2,4,6-TBP in the urine of Chilean sawmills workers, which was 6.9 mg/g creatinine (approx.…”

Section: Introductionmentioning

confidence: 92%

Genotoxic Mechanism of Action of TBBPA, TBBPS and Selected Bromophenols in Human Peripheral Blood Mononuclear Cells

et al. 2022

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Bromophenolic flame retardants (BFRs) are a large group of synthetic substances used in the industry in order to reduce the flammability of synthetic materials used in electrical and electronic devices, textiles, furniture and other everyday products. The presence of BFRs has been documented in the environment, food, drinking water, inhaled dust and the human body. Due to the widespread exposure of the general population to BFRs and insufficient knowledge on their toxic action, including genotoxic potential, we have compared the effect of tetrabromobisphenol A (TBBPA), tetrabromobisphenol S (TBBPS), 2,4,6,-tribromophenol (2,4,6-TBP) and pentabromophenol (PBP) on DNA damage in human peripheral blood mononuclear cells (PBMCs) (playing a crucial role in the immune system) as well as examined underlying mechanism of action of these substances. The cells were incubated for 24 h with studied compounds in the concentrations ranging from 0.01 to 10 µg/mL. The study has shown that examined BFRs induced single and, to a lesser extent, double strand-breaks formation and caused oxidative damage to pyrimidines, and particularly to purines in the incubated cells. PBMCs efficiently repaired the DNA strand-breaks induced by BFRs, but they were unable to remove completely damaged DNA (except cells treated with TBBPS). The greatest changes in the above-mentioned parameters were observed in cells incubated with TBBPA, while the smallest in PBMCs treated with TBBPS. The results have also revealed that tested compounds do not form adducts with DNA in PBMCs, while the observed changes were the most probably induced by indirect DNA-damaging agents, such as ROS and other reactive species.

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“…The relatively high MDLs of these "subordinate" BPs, such as the PBP with MDL up to 46 and 106 ng/L [27,28], made the methods inappropriate to determine the trace levels of BPs in environmental aqueous samples. To our knowledge, there is only one recent report which specially studied BPs in human urine using LLE-SPE-GC/MS method [31]. However, the method detection limit of 1.8-22.9 ng/L and recovery of 36-152% for BPs is not satisfactory enough, especially for the analysis of pollutants at environmental low levels.…”

Section: Introductionmentioning

confidence: 99%

Analysis of bromophenols in various aqueous samples using solid phase extraction followed by HPLC-MS/MS

Chi

Liu

et al. 2017

Talanta

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A reliable and effective method for simultaneous analysis of trace amount of bromophenols (BPs) in various aqueous samples was developed in this study. The aqueous samples must be acidified after sampling to prevent the fast degradation of bromophenols. Solid-phase extraction was chosen to extract and purify the water samples. High performance liquid chromatography coupled with tandem mass spectrometry with an electrospray ionization source (HPLC-ESI-MS/MS) was used for following identification and quantification. Under the optimized condition, fourteen out of fifteen target brominated phenols were successfully separated and detected with the exception of 2,6-bromophenol (2,6-BP) whose response was too low to be quantified by the MS detector. The method was validated by spiking river water and seawater samples with different concentrations of BPs, and the qualified spiking recoveries (64-100%) and precisions (0.4-11% RSD) were obtained. The method detection limits were 0.1-13.9ng/L and 0.1-21.9ng/L for 0.1L of river water and seawater samples, respectively, showing the influences from the sample matrix. The proposed method was successfully applied to the analysis of bromophenol contamination in real water samples, and six bromophenols were identified with a wide concentrations ranging from ng/L to μg/L.

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Determination of urinary bromophenols (BrPs) as potential biomarkers for human exposure to polybrominated diphenyl ethers (PBDEs) using gas chromatography-tandem mass spectrometry (GC–MS/MS)

Cited by 33 publications

References 12 publications

Urinary Volatomic Expression Pattern: Paving the Way for Identification of Potential Candidate Biosignatures for Lung Cancer

Urinary Volatomic Expression Pattern: Paving the Way for Identification of Potential Candidate Biosignatures for Lung Cancer

Genotoxic Mechanism of Action of TBBPA, TBBPS and Selected Bromophenols in Human Peripheral Blood Mononuclear Cells

Analysis of bromophenols in various aqueous samples using solid phase extraction followed by HPLC-MS/MS

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