Determination of volatile organic compounds by HS‐GC‐IMS to detect different stages of <i>Aspergillus flavus</i> infection in Xiang Ling walnut

Wang, Shan; Mo, Haizhen; Xu, Dan; Hu, Huiling; Hu, Liangbin; Shuai, Liang; Li, Hongbo

doi:10.1002/fsn3.2229

Cited by 12 publications

(9 citation statements)

References 37 publications

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“…Volatile organic compounds determination in the early stage of pathogen infection was found to be useful in preventing risk of mycotoxin production on wheat [11] and walnut [12] by toxicogenic Aspergillus, Fusarium, and Penicillium species. Josselin and co-workers [13] distinguished crop infection with A. flavus strain (aflatoxin B1 producer) from non-toxicogenic strain by mVOCs evaluation.…”

Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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The feasibility of early disease detection in potato seeds storage monitoring of volatile organic compounds (VOCs) and plant physiological markers was evaluated using 10 fungal and bacterial pathogens of potato in laboratory-scale experiments. Data analysis of HS-SPME-GC-MS revealed 130 compounds released from infected potatoes, including sesquiterpenes, dimethyl disulfide, 1,2,4-trimethylbenzene, 2,6,11-trimethyldodecane, benzothiazole, 3-octanol, and 2-butanol, which may have been associated with the activity of Fusarium sambucinum, Alternaria tenuissima and Pectobacterium carotovorum. In turn, acetic acid was detected in all infected samples. The criteria of selection for volatiles for possible use as incipient disease indicators were discussed in terms of potato physiology. The established physiological markers proved to demonstrate a negative effect of phytopathogens infecting seed potatoes not only on the kinetics of stem and root growth and the development of the entire root system, but also on gas exchange, chlorophyll content in leaves, and yield. The negative effect of phytopathogens on plant growth was dependent on the time of planting after infection. The research also showed different usefulness of VOCs and physiological markers as the indicators of the toxic effect of inoculated phytopathogens at different stages of plant development and their individual organs.

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Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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“…In addition to various advantages and disadvantages, it seems that one other character that gives added value is the ability of Aspergillus in general to produce volatile organic compounds (VOCs). VOCs are hydrocarbons with various molecules of acids, alcohols, aldehydes, aromatics, ketones, terpenes, thiols, and their derivatives that have ecological functions [43] and are unique to the genus Aspergillus [44]. These VOCs are closely related to the role of these fungi in bioconversion processes such as composting and weathering of organic matter as well as intra-and inter-organism communication processes such as promoting plant growth and signaling germination for fungi [45], so that these fungi will encourage interactions positive for the biological quality in the rhizosphere which is a characteristic of bioferilizer biological agents.…”

Section: Resultsmentioning

confidence: 99%

Identification of Several Aspergillus Isolates Candidates for Bio Fertilizer Agents Using Molecular Markers

Sutarman

2022

IOP Conf. Ser.: Earth Environ. Sci.

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This research is an activity to characterize and determine several types of soil-borne fungi based on the use of DNA sequences. This study aims to determine the species name and genealogy of four selected fungal isolates from the genus Aspergillus isolated from horticultural fields in Bumiaji, Batu, East Java. The identification of biocontrol agents begins with macroscopic and microscopic morphological observations, followed by DNA isolation, polymerase chain reaction application using ITS 1 and ITS 4 primers. Sequencing is done using Sanger sequencing. The nucleotides generated from the sequencer machine (ABI 3730XL sequencer) were compared with the gene bank using the BLAST-NCBI program, and their relationships were analyzed using MEGA X software. The results showed that based on BLAST analysis of the nucleotide sequences of Aspergilus spp. isolates 6, 7, 17, and 21 were compatible with Aspergillus tamarii with 100% Query Cover and 100% identical rate. These four Aspergillus isolates have the potential as bofertilizer agents and can be used for various industrial purposes.

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“…In this experiment Aspergillus also supported plant growth (Tables 2, 3, and 4); This is in line with the ability of this fungus to produce various volatile organic compounds such as various molecules of acids, alcohols, aldehydes, aromatics, ketones, terpenes, thiols, and their derivatives which make them have an ecological advantage in their niche as well as provide growth support for plants. [35][36].…”

Section: Discussionmentioning

confidence: 99%

Application of Biological Agents of Trichoderma and Aspergillus on Cayenne Chilli Plants in Endemic Land with Fusarium Wilt

Farihadina

Sutarman

2022

IOP Conf. Ser.: Earth Environ. Sci.

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This study aims to determine the inhibitory power of the biological agent Trichoderma sp. Tc-Sb-11 and Aspergillus sp. As-Sb-15 in vitro against Fusarium oxysporum Fo-Sl-02 and the effect of its application by soil treatment and apical treatment on cayenne pepper grown on fusarium wilt endemic land. Inhibition research was carried out by placing each biological agent propagule in front of the pathogenic propagule in dual culture and measuring the percentage of inhibition against the pathogen. Experimental application of biological agents was carried out in Seloliman village (Trawas, Mojokerto) with each treatment applied by soil treatment and apical treatment and without application of biological agents (control). The variables observed were the percentage of disease incidence, flower growing time, fruit growing time, and fruit weight. All data were analyzed by ANOVA at the 5% level followed by a DMRT test to determine the difference in the effect of treatment. The results showed that the application of Trichoderma sp. and Aspergillus sp. in vitro inhibited the growth of F. oxysporum colonies 95.6 ± 0.8 and 30.7 ± 4.5%, respectively. Trichoderma which was applied by soil treatment could reduce the incidence of the highest disease up to 58.7%, accelerate the breeding age up to 4.93%, and accelerate fruit growth up to 5.21%. The application of Trichoderma and Aspergillus by apical treatment increased fruit weight per plant by 58.36 and 49.34%, respectively.

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Determination of volatile organic compounds by HS‐GC‐IMS to detect different stages of Aspergillus flavus infection in Xiang Ling walnut

Cited by 12 publications

References 37 publications

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Identification of Several Aspergillus Isolates Candidates for Bio Fertilizer Agents Using Molecular Markers

Application of Biological Agents of Trichoderma and Aspergillus on Cayenne Chilli Plants in Endemic Land with Fusarium Wilt

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