Determined GA by FI‐CE in Luminol‐H<sub>2</sub>O<sub>2</sub> System

Dong, Yang; Wang, Zhongling

doi:10.1002/jccs.201200086

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CL mechanism of Co 2+ remarkably enhancing luminol intensity has been well studied [19, 20], and a variety of applications with luminol-Co 2+ CL system in trace analysis [21, 22] have been reported. For example, it has been applied to the determination of hydrogen peroxide [23, 24], acetaminophen [25], lanthanides [26], carbaryl [27], captopril [28], vitamin B 12 [29], gibberellic acid [30], and radical scavenging potential of ascorbic acid [31]. With luminol-H 2 O 2 -Co 2+ CL system, the determination of UA have been reported [32], while no real-time analysis of urine UA excretion with luminol-Co 2+ CL system has been described so far.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Wang

Tan

Song

2013

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+ chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Wang

Tan

Song

2013

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

Picomole‐level Formaldehyde Determination in Gaseous and Beer Samples Using Flow Injection Chemiluminescence Analysis

Tan

Song

2013

J Chinese Chemical Soc

View full text Add to dashboard Cite

Based on the linear enhancement of formaldehyde (FA) within 7.0~1000 pmol l -1 on luminol-bovine serum albumin (BSA) chemiluminescence (CL) system, FA determination in air and beer samples using CL with flow injection (FI) was proposed. The detection limit was 2.5 pmol l -1 (3s) and the relative standard deviations were less than 4.5% (n = 7). At a flow rate of 2.0 mL min -1 , a whole analysis from sampling to washing only needed 32 s, offering a sample throughput of 112 h -1 . This proposed method was successfully utilized to determine FA vapor pressure in liquid (121.8 ± 3.8 Pa), FA content in real air sample (8.93 ± 0.03 mg m -3 ), and FA levels in beer (199.5 ± 5.6~225.2 ± 3.5 mg l -1 ), giving determination recoveries from 90.7% to 109.3%. The mechanism of BSA-FA interaction was also investigated, showing FA binding to BSA was a spontaneous process mainly through hydrogen bonding and van der Waals force by FI-CL, with binding constant K of 1.89 × 10 6 l mol -1 and the number of binding sites n of 0.86. Molecular docking analysis further revealed FA could enter into the pocket at subdomain IIA of BSA, with K of 1.71 × 10 5 l mol -1 and DG of -29.68 kJ mol -1 .Keywords: Formaldehyde; Vapor pressure; Real air sample; Beer samples; Bovine serum albumin; Luminol; FI-CL. INTRODUCTIONFormaldehyde (FA), a colorless pungent chemical with high water solubility, is usually released as a fixative for pathologists, a byproduct of combustion and off-gassing of various building products, leading to wide existence in foods, industry and environment.1,2 It has attracted intensive attention in public 3-5 because of its negative impacts on human health. Chemiluminescence (CL) in combination with FI technique has also been favorably utilized in FA determination, 42-44 due to its conspicuous merits in terms of high sensitivity, low limit of detection (LOD), wide dynamic concentration response range, automatability and less reagent consumption. [45][46][47][48][49] However, so far no FI-CL using luminol-bovine serum albumin (BSA) system has been described for FA determination. In this current work, it was found that FA can remarkably enhance CL intensity from luminol-BSA system, and the CL intensity increments were linear with FA concentrations from 7.0 to 1000 pmol l -1 . Based on the good linear enhancement, a simple FI-CL method using luminol-BSA system was first developed to determine FA. RESULTS AND DISCUSSIONThe relative CL intensity-time profile of luminol-BSA-FA system The relative CL intensity-time profile of luminol-BSA-FA system was given in Fig. 4 (I max referred to the maximum CL intensity, and T max referred to the time for the I max ). The studied concentrations of luminol, BSA and FA were 5.0 × 10 -5 , 5.0 × 10 -9 and 3.0 × 10 -10 mol l -1 , respectively. It can be seen that the I max of luminol-BSA system (curve ¡) is 219 with the T max of 4.1 s, and then vanishes in the following 28 s; however in the presence of FA (curve l), the I max of luminol-BSA system strikingly increases about 1.92-fold to 420 with the...

show abstract

Determined GA by FI‐CE in Luminol‐H₂O₂ System

Cited by 2 publications

References 10 publications

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Picomole‐level Formaldehyde Determination in Gaseous and Beer Samples Using Flow Injection Chemiluminescence Analysis

Contact Info

Product

Resources

About

Determined GA by FI‐CE in Luminol‐H2O2 System

Cited by 2 publications

References 10 publications

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Picomole‐level Formaldehyde Determination in Gaseous and Beer Samples Using Flow Injection Chemiluminescence Analysis

Contact Info

Product

Resources

About

Determined GA by FI‐CE in Luminol‐H₂O₂ System