Determining different impact factors on the xylonic acid production using Gluconobacter oxydans DSM 2343

Hahn, Thomas; Torkler, S.; Bolt, R. van der; Gammel, N.; Hesse, Michelle; Møller, Anders Hauer; Preylowski, B.; Hubracht, V.; Patzsch, Katja; Hahn, Thomas

doi:10.1016/j.procbio.2020.04.011

Cited by 21 publications

(15 citation statements)

References 23 publications

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“…For instance, XA can be applied for food, pharmaceutical, and agricultural purposes [ 39 ], it can be used as sustainable solvent and biocatalyst [ 40 ] or as from waste biomass derived polymer precursor [ 41 ]. In literature microbial conversion of xylose to XA is reported by Kluyveromyces lactis , Aspergillus niger, Gluconobacter oxydans, or recombinant Saccharomyces cerevisiae and Escherichia coli [ 39 , 41 , 42 ]. The XA production ability of S. podzolica was to our knowledge never described before.…”

Section: Discussionmentioning

confidence: 99%

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte

Kugel

Ochsenreither

2020

Biotechnol Biofuels

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Background Biotechnologically produced microbial lipids are of interest as potential alternatives for crude and plant oils. Their lipid profile is similar to plant oils and can therefore be a substitute for the production of biofuels, additives for food and cosmetics industry as well as building blocks for oleochemicals. Commercial microbial lipids production, however, is still not profitable and research on process optimization and cost reduction is required. This study reports on the process optimization using glucose or xylose with the unconventional oleaginous yeast Saitozyma podzolica DSM 27192 aiming to reduce the applied carbon source amount without sacrificing lipid productivity. Results By optimizing the process parameters temperature and pH, lipid productivity was enhanced by 40%. Thereupon, by establishing a two-phase strategy with an initial batch phase and a subsequent fed-batch phase for lipid production in which a constant sugar concentration of about 10 g/L was maintained, resulted in saving of ~ 41% of total glucose and ~ 26% of total xylose. By performing the automated continuous sugar feed the total sugar uptake was improved to ~ 91% for glucose and ~ 92% for xylose and thus, prevented waste of unused carbon source in the cultivation medium. In addition, reduced glucose cultivation resulted in to 28% higher cell growth and 19% increase of lipid titer. By using xylose, the by-product xylonic acid was identified for the first time as by-product of S. podzolica. Conclusions These findings provide a broad view of different cultivation process strategies with subsequent comparison and evaluation for lipid production with S. podzolica. Additionally, new biotechnological characteristics of this yeast were highlighted regarding the ability to produce valuable organic acids from sustainable and renewable sugars.

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Section: Discussionmentioning

confidence: 99%

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte

Kugel

Ochsenreither

2020

Biotechnol Biofuels

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“…AAB strains can also transform d -arabinose to 4-keto- d -arabinose [35] and 4-keto- d -arabonate [36] (Adachi et al, 2010 ); d -ribose to 4-keto- d -ribose [41] and 4-keto- d -ribonate [42] (Adachi et al, 2011a ); xylose to xylonic acid [43] (Buchert et al, 1988 ; Hahn et al, 2020 ), d -xylulose to xylitol [44] (Qi et al, 2016 ), sorbose to sorbitol [45] and 2-keto- d -gulonic acid [46] (Sugisawa et al, 1991 ; Adachi et al, 1999a ), and d -galactose to d -galactonic acid [47] (Švitel and Šturdik, 1994 ). Adachi et al ( 2013 ) also found that AAB cells could use 2-deoxy- d -ribose as a substrate to produce 2-deoxy-4-keto- d -ribose [48] and 2-deoxy-4-keto- d -ribonate [49] , which are under the action of membrane-binding d -aldopentose 4-dehydrogenase and 4-keto- d -1-dehydrogenase, respectively (Adachi et al, 2013 ).…”

Section: Natural Products Yielded By Aofmentioning

confidence: 99%

Oxidative Fermentation of Acetic Acid Bacteria and Its Products

Xie

Zhang

et al. 2022

Front. Microbiol.

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Acetic acid bacteria (AAB) are a group of Gram-negative, strictly aerobic bacteria, including 19 reported genera until 2021, which are widely found on the surface of flowers and fruits, or in traditionally fermented products. Many AAB strains have the great abilities to incompletely oxidize a large variety of carbohydrates, alcohols and related compounds to the corresponding products mainly including acetic acid, gluconic acid, gulonic acid, galactonic acid, sorbose, dihydroxyacetone and miglitol via the membrane-binding dehydrogenases, which is termed as AAB oxidative fermentation (AOF). Up to now, at least 86 AOF products have been reported in the literatures, but no any monograph or review of them has been published. In this review, at first, we briefly introduce the classification progress of AAB due to the rapid changes of AAB classification in recent years, then systematically describe the enzymes involved in AOF and classify the AOF products. Finally, we summarize the application of molecular biology technologies in AOF researches.

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“…However, the much lower productivity compared to G. oxydans is still a serious weakness. Hahn et al (2020) reported the effect of different nitrogen sources on the growth and subsequent xylonic acid production. With the addition of 0.32 g L −1 glutamate and 0.15 g L −1 ammonium sulfate as inexpensive nitrogen sources, a xylonic acid volumetric productivity of 2.92 g L −1 h −1 is obtained.…”

Section: A Comparison Of Specific Productivity For Xylonic Acid Fermentation In the Literaturementioning

confidence: 99%

Optimization of Specific Productivity for Xylonic Acid Production by Gluconobacter oxydans Using Response Surface Methodology

Ding

et al. 2021

Front. Bioeng. Biotechnol.

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Large amounts of xylose cannot be efficiently metabolized and fermented due to strain limitations in lignocellulosic biorefinery. The conversion of xylose into high value chemicals can help to reduce the cost of commercialization. Therefore, xylonic acid with potential value in the construction industry offers a valuable alternative for xylose biorefinery. However, low productivity is the main challenge for xylonic acid fermentation. This study investigated the effect of three reaction parameters (agitation, aeration, and biomass concentration) on xylose acid production and optimized the key process parameters using response surface methodology The second order polynomial model was able to fit the experimental data by using multiple regression analysis. The maximum specific productivity was achieved with a value of 6.64 ± 0.20 g gx−1 h−1 at the optimal process parameters (agitation speed 728 rpm, aeration rate 7 L min−1, and biomass concentration 1.11 g L−1). These results may help to improve the production efficiency during xylose acid biotransformation from xylose.

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Determining different impact factors on the xylonic acid production using Gluconobacter oxydans DSM 2343

Cited by 21 publications

References 23 publications

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Oxidative Fermentation of Acetic Acid Bacteria and Its Products

Optimization of Specific Productivity for Xylonic Acid Production by Gluconobacter oxydans Using Response Surface Methodology

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