Pesticide residues are one of the most dangerous things that can affect the safety and quality of agro-food. We assessed study participants' long-term dietary exposure to 17 OPs and looked at how conventional food consumption and OP urinary metabolites in young children's and adolescents' urine samples impacted this exposure. We investigate the OP pesticide and metabolite concentrations in the study participants' diet and urine for the extraction and quantitative analyses of pesticides by using the QuEChERS method & followed by the LC-MS/MS. We gathered the food intake data of around 6–15-year-old (N = 905) child volunteers based on parent interviews and food questionnaires from the study proforma and also collected their urine samples. The health risk assessments through EDI, ADI, and chronic or long-term health (HQ) were estimated. Our results suggest that 17 OP pesticides were found in food samples. Among 905 study participants based on diet, there was a significant (p < 0.000) difference in the consumption of fruits & vegetables between the males and females, sex-wise, and the adolescents and child, age-wise. We investigated the DAP levels in urine samples, and there was a significant (p < 0.05) difference in the concentration of DAPs levels between the child female, & HIG groups, which showed more DAP levels than the child male, LIG, & MIG groups. The analysis of EDI and health quotient (HQ) values of pesticides on study participants showed that pesticides such as dichlorvos, monocrotophos, dimethoate, quinalphos, triazophos, and ethion were significantly associated with the health risks of children through their diets. The findings indicate that there is more risk associated with fruits, fruit juices, and vegetables compared to plain foods, but future total exposure to these toxins from a variety of foods warrants extra vigilance.