“…Master mixes were split to glass vials with 500 μl reaction volume each. To initiate the reaction different substrate concentrations (1,5,20,30,50,80, 100, 150 μM) were added, reaction mixtures were overlaid with 150 μl of isooctane and incubated at 34 C for 2, 4, 6, 8, and 10 min. To stop the reaction, the mixture was shaking for 30 s and centrifuged for 5 min at 14,000 rpm and room temperature.…”