Determining the Relationship Between Homocysteinemia and Biomarkers of Inflammation, Oxidative Stress and Functional Kidney Status in Patients with Diabetic Nephropathy

SummaryBackgroundThe aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among these parameters and to evaluate potential differences in all the biomarkers with regard to the immunosuppression protocol.MethodsWe enrolled 57 renal transplant recipients and 31 controls who were age and sex matched with the renal transplant recipients. All of the patients included in this study had post-renal transplant surgery at least 12 months earlier and were on standard immunosuppressive therapy. In this study, we determined thiobarbituric acid-reactive substances in plasma and red blood cells and advanced oxidation protein products, nitrosative stress parameters (asymmetric and symmetric dimethylarginine – ADMA and SDMA), and antioxidative parameters (total SH groups and catalase activity).ResultsThe results of our study demonstrated that the levels of oxidative and nitrosative stress were significantly increased compared to the healthy population (p<0.01 except for plasma catalase activity p<0.05). Correlation analysis showed significant positive correlations between: ADMA and SDMA (p<0.01); ADMA and nitrates (p<0.05); SDMA and nitrates (p<0.05); between OS parameters in the experimental group; AOPP and SH groups (p<0.05) and TBARS in plasma and SH groups (p<0.01), SDMA and AOPP (p< 0.05); SDMA and TBARS in plasma (p<0.05); SDMA and SH groups (p<0.01); nitrates and SH groups (p<0.05).ConclusionThere was no significant difference in oxidative and nitrosative stress parameters with respect to the immunosuppressive protocol.

Section: Discussionmentioning

confidence: 92%

Oxidative and Nitrosative Stress in Stable Renal Transplant Recipients with Respect to the Immunosuppression Protocol – Differences or Similarities? / Oksidativni I Nitrozativni Stres U Odnosu Na Imunosupresivni Protokol Kod Pacijenata Sa Stabilnom Funkcijom Presađenog Bubrega – Razlike I Sličnosti

Cvetković

Veličković‐Radovanović

Stojanović

et al. 2015

“…An increase in HbA1c indicated a poor control of diabetic patients, and treatment was inadequate or diet control was very poor for two months to four months ( 16 ). Excessive glycosylation of various proteins has been considered as a major pathogenic factor in microvascular and neuropathic complications of diabetes ( 17 , 18 ). Dyslipidemia observed in the patients included in this study is common in diabetic patients and can be attributed to different factors ( 19 , 20 ).…”

Section: Discussionmentioning

confidence: 99%

Correlation Between Glycated Hemoglobin and Homa Indices in Type 2 Diabetes Mellitus: Prediction of Beta-Cell Function from Glycated Hemoglobin / Korelacija Između Glikoliziranog Hemoglobina I Homa Indeksa U Dijabetes Melitusu Tipa 2: Predviđanje Funkcije Beta Ćelija Na Osnovu Glikoliziranog Hemoglobina

Al‐Hakeim¹,

Abdulzahra²

2015

SummaryBackgroundThe present study aimed to determine the most efficient insulin resistance function related to glycemic control expressed as glycated hemoglobin (HbA1c) in type 2 diabetes mellitus patients (T2DM). The other aim is to derive equations for the prediction of beta cell functions containing HbA1c as a parameter in addition to fasting glucose and insulin.MethodsT2DM Patients were grouped according to the following: (1) degree of control (good, fair, and poor control) and (2) insulin resistance as observed in obtained data and significant differences revealed by the homeostasis model assessment (HOMA) of related parameters (insulin resistance = HOMA2IR, beta-cell function = HOMA%B, and insulin sensitivity = HOMA%S) among groups. Correlations and forecasting regression analysis were calculated.ResultsHbA1c was found to be correlated with insulin resistance parameters in T2DM subgroups. This correlation was also significantly correlated with HOMA%B and the quantitative insulin sensitivity check index (QUICKI) in fair and poor control groups. Regression analysis was used to predict the forecasting equations for HOMA%B. The best applicable equations were derived for healthy control (HOMA2%B=−1.76*FBG+5.00*Insulin+4.69*HbA1c+189.84) and poor control groups (HOMA2%B=0.001* FBG+0.5*Insulin-8.67*HbA1c+101.96). These equations could be used to predict β-cell function (HOMA%B) after FBG, insulin and HbA1c values were obtained for healthy and poor control groups. In the good and fair control groups, the applicability of the HOMA model fails to yield appropriate results.ConclusionsBeta-cell function is correlated with QUICKI and HbA1c and could be predicted properly from HbA1c, insulin, and glucose in the healthy and poor control groups. New regression equations were established that involve HbA1c.

“…These findings indicate that the replenishment To date, an agreement on the correlation between HHcy and cognitive function has not been reached. Some scholars believe that HHcy serves as an independent risk factor of cognitive disorder or a decrease in cognitive function (16,17), whereas some hold that HHcy has nothing to do with cognitive function (19). To the formers two channels through which HHcy leads to cognitive disorder are believed to exist.…”

Section: Discussionmentioning

confidence: 99%

Intervention Effect of Folic Acid and Vitamin B12 on Vascular Cognitive Impairment Complicated With Hyperhomocysteinemia / Efekat Intervencije Folnom Kiselinom I Vitaminom B12 Na Vaskularni Kognitivni Poremećaj Komplikovan Hiperhomocisteinemijom

Jiang¹,

Cheng-yun²,

Yao³

et al. 2013

SummaryBackground: Hyperhomocysteinemia (HHcy) may be correlated with cognitive function. Although intervention with folic acid and VitB 12 can decrease the homocysteine (Hcy) level, its effect on cognitive function remains uncertain. This prospective study aimed to explore the effects of folic acid and VitB 12 on the Hcy level and cognitive function in patients with vascular cognitive impairment-no dementia (VCIND) complicated with HHcy. Methods: A total of 120 patients with VCIND complicated by HHcy were randomly selected. They were divided into intervention and control groups. The intervention group was given 5 mg of folic acid per day and 500 mg of VitB 12 thrice per day apart from conventional therapy. Folic acid, VitB 12 , and Hcy were determined and Montreal cognitive assessment (MoCA) and event-related potential P300 determination were performed before and after treatment. Results: Before treatment, no significant differences in the folic acid, VitB 12 , Hcy, MoCA, and P300 parameters were observed between the groups. After treatment, the folic acid and VitB 12 levels increased and the Hcy level decreased in the intervention group compared with that before treatment and in the control group. At 24 weeks, the MoCA score and P300 outcomes in the intervention group improved compared with those before treatment and in the control group. Conclusions: Folic acid and VitB 12 effectively decrease the Hcy level in VCIND patients and improve their cognitive functions.