Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

Bittner, Andras; Griebel, Thomas; Buer, Jörn van; Juszczak-Debosz, Ilona; Baier, Margarete

doi:10.1007/978-1-0716-0660-5_16

Cited by 7 publications

(8 citation statements)

References 44 publications

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“…Medium old leaves, which show strongest priming sensitivity [30] and lowest background H 2 O 2 levels [92], were frozen immediately in liquid nitrogen to fix metabolism. 50 mg of the frozen plant material was homogenized in 200 μl 5 mM KCN according to [93].…”

Section: Quantification Of H 2 O 2 Levelsmentioning

confidence: 99%

Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

2020

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Background: The majority of stress-sensitive genes responds to cold and high light in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4°C cold stimulus modifies cold regulation of gene expression for up to a week at 20°C, although the primary cold effects are reverted within the first 24 h. Such memory-based regulation is called priming. Here, we analyse the effect of 24 h cold priming on cold regulation of gene expression on a transcriptomewide scale and investigate if and how cold priming affects light regulation of gene expression. Results: Cold-priming affected cold and excess light regulation of a small subset of genes. In contrast to the strong gene coregulation observed upon cold and light stress in non-primed plants, most priming-sensitive genes were regulated in a stressor-specific manner in cold-primed plant. Furthermore, almost as much genes were inversely regulated as co-regulated by a 24 h long 4°C cold treatment and exposure to heat-filtered high light (800 μmol quanta m − 2 s − 1). Gene ontology enrichment analysis revealed that cold priming preferentially supports expression of genes involved in the defence against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defence genes in response to high light triggering. qPCR with independently cultivated and treated replicates confirmed the trends observed in the RNASeq guide experiment. Conclusion: A 24 h long priming cold stimulus activates a several days lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defence regulation in a stressor-specific manner.

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Section: Quantification Of H 2 O 2 Levelsmentioning

confidence: 99%

Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

2020

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“…Additionally, cytosolic thioredoxins h -type may also participate in regulation of seed germination process, whereas thioredoxins f -type may also be involved in starch accumulation [ 28 , 32 ]. Oxidized thioredoxins undergo reduction reaction catalyzed by two types of thioredoxin reductases (i.e., ferredoxin–thioredoxin reductase and NADPH-dependent thioredoxin reductase) [ 33 , 34 ]. The efficient functioning of Trx/TrxR systems in the plant tissues contributes to maintaining redox balance in the cells under physiological and unfavorable environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings

Sytykiewicz

Łukasik

Goławska

et al. 2020

IJMS

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Thioredoxins (Trxs) and thioredoxin reductases (TrxRs) encompass a highly complex network involved in sustaining thiol-based redox homeostasis in plant tissues. The purpose of the study was to gain a new insight into transcriptional reprogramming of the several genes involved in functioning of Trx/TrxR system in maize (Zea mays L.) seedlings, exposed to the bird cherry-oat aphid (Rhopalosiphum padi L.) or the rose-grass aphid (Metopolophium dirhodum Walk.) infestation. The biotests were performed on two maize genotypes (susceptible Złota Karłowa and relatively resistant Waza). The application of real-time qRT-PCR technique allowed to identify a molecular mechanism triggered in more resistant maize plants, linked to upregulation of thioredoxins-encoding genes (Trx-f, Trx-h, Trx-m, Trx-x) and thioredoxin reductase genes (Ftr1, Trxr2). Significant enhancement of TrxR activity in aphid-infested Waza seedlings was also demonstrated. Furthermore, we used an electrical penetration graph (EPG) recordings of M. dirhodum stylet activities in seedlings of the two studied maize varieties. Duration of phloem phase (E1 and E2 models) of rose-grass aphids was about three times longer while feeding in Waza plants, compared to Złota Karłowa cv. The role of activation of Trx/TrxR system in maintaining redox balance and counteracting oxidative-induced damages of macromolecules in aphid-stressed maize plants is discussed.

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“…To analyse hydrogen peroxide abundance after cold exposure and B. cinerea infection, 3,3′-diaminobenzidine (DAB) was used as previously described (Bittner et al, 2020). 1 mg ml -1 DAB was dissolved in phosphate-buffered saline (PBS; 73 mM NaCl, 10 mM, Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4, pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

“…The background of stained leaves was removed in a 1:1:3 mixture of acetic acid, glycerol, and ethanol before analysis and image acquisition. The intensity of DAB staining per leaf was calculated using ImageJ (Schneider et al, 2012; Bittner et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Cold exposure transiently increases resistance ofArabidopsis thalianaagainst the fungal pathogenBotrytis cinerea

Schütte,

Remmo,

Baier

et al. 2024

Preprint

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A sudden cold exposure (4°C, 24 h) primes resistance ofArabidopsis thalianaagainst the virulent biotrophic pathogenPseudomonas syringaepv.tomatoDC3000 (Pst) for several days. This effect is mediated by chloroplast cold sensing and the activity of stromal and thylakoid-bound ascorbate peroxidases (sAPX/tAPX). In this study, we investigated the impact of such cold exposure on plant defence against the necrotrophic fungusBotrytis cinerea. Plant resistance was transiently enhanced if theB. cinereainfection occurred immediately after the cold exposure, but this cold-enhancedB. cinerearesistance was absent when the cold treatment and the infection were separated by 5 days at normal growth conditions. Plastid ascorbate peroxidases partially contributed to the transient cold-enhanced resistance against the necrotrophic fungus. In response toB. cinerea, the levels of reactive oxygen species (ROS) were significantly higher in cold-pretreated Arabidopsis leaves. Pathogen-triggered ROS levels varied in the absence of sAPX, highlighting the strong capacity for sAPX-dependent ROS regulation in the chloroplast stroma. The cold-enhanced resistance againstB. cinereawas associated with cold-induced plant cell wall modifications, including sAPX-dependent callose formation and significant lignification in cold-treated Arabidopsis leaves.FundingThis work was supported by the German Research Foundation (CRC973/C4) and the FU Berlin.

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Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

Cited by 7 publications

References 44 publications

Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings

Cold exposure transiently increases resistance ofArabidopsis thalianaagainst the fungal pathogenBotrytis cinerea

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