Determining the Structure of Hexametaphosphate by Titration and <sup>31</sup>P-NMR Spectroscopy

Robinson, Thomas E.; Arkinstall, Lucy A.; Cox, Sophie C.; Grover, Liam M.

doi:10.1080/02603594.2021.1973444

Cited by 7 publications

(1 citation statement)

References 29 publications

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“…[9][10][11][12] In this study, a novel strategy was employed by utilizing a composite cross-linking agent of tripolyphosphate (TPP) and sodium hexametaphosphate (HMP) for the ion cross-linking of alginate, resulting in the fabrication of alginate hydrogels. [13][14][15][16] Hydrogels, with their high water content and three-dimensional network structure, are well-suited for drug delivery applications due to their ability to encapsulate and release therapeutic agents in a controlled manner. [1,[17][18][19][20] To comprehensively evaluate the performance of the alginate hydrogel hydrogels, various parameters were investigated.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Swelling Analysis of Alginate Hydrogel

Toleuova

2024

Preprint

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Utilizing a combination of sodium tripolyphosphate (TPP) and sodium hexametaphosphate (HMP) as composite cross-linking agents, alginate hydrogels were prepared through ionic cross-linking. An analysis and discussion were conducted on the influence of crosslinking agent mass ratio, pH, and ionic strength on the swelling properties of alginate hydrogels. The findings reveal that the swelling degree of microspheres prepared with the composite crosslinking agent is reduced by 75.1% and 26.8% compared to using TPP or HMP separately, indicating a significantly enhanced cross-linking efficiency. When the mass ratio of TPP to HMP is set at 4:5 and the cross-linking agent pH is 6, the hydrogels demonstrated the utmost crosslinking and the most compact structure. In a pH 2.0 buffered solution, the swelling degree can reach 500%, and the gel remains intact without any breakage. The hydrogels exhibit notable sensitivity to both ionic strength and pH, displaying increased swelling particularly in acidic conditions.

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Section: Introductionmentioning

confidence: 99%

Comprehensive Swelling Analysis of Alginate Hydrogel

Toleuova

2024

Preprint

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Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7

Deitert,

Fees,

Mertens

et al. 2024

Yeast

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Polyphosphate (polyP) is an intriguing molecule that is found in almost any organism, covering a multitude of cellular functions. In industry, polyP is used due to its unique physiochemical properties, including pH buffering, water binding, and bacteriostatic activities. Despite the importance of polyP, its analytics is still challenging, with the gold standard being 31P NMR. Here, we present a simple staining method using the fluorescent dye JC‐D7 for the semi‐quantitative polyP evaluation in yeast extracts. Notably, fluorescence response was affected by polyP concentration and polymer chain length in the 0.5–500 µg/mL polyP concentration range. Hence, for polyP samples of unknown chain compositions, JC‐D7 cannot be used for absolute quantification. Fluorescence of JC‐D7 was unaffected by inorganic phosphate up to 50 mM. Trace elements (FeSO4 > CuSO4 > CoCl2 > ZnSO4) and toxic mineral salts (PbNO3 and HgCl2) diminished polyP–induced JC‐D7 fluorescence, affecting its applicability to samples containing polyP–metal complexes. The fluorescence was only marginally affected by other parameters, such as pH and temperature. After validation, this simple assay was used to elucidate the degree of polyP production by yeast strains carrying gene deletions in (poly)phosphate homeostasis. The results suggest that staining with JC‐D7 provides a robust and sensitive method for detecting polyP in yeast extracts and likely in extracts of other microbes. The simplicity of the assay enables high‐throughput screening of microbes to fully elucidate and potentially enhance biotechnological polyP production, ultimately contributing to a sustainable phosphorus utilization.

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Acidity Constant Estimation of Weakly Acidic Polyelectrolyte by Linear Approximation: A Case Study for Polyphosphate via Gran’s Approach

Cini

2023

J Solution Chem

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Determining the Structure of Hexametaphosphate by Titration and ³¹P-NMR Spectroscopy

Cited by 7 publications

References 29 publications

Comprehensive Swelling Analysis of Alginate Hydrogel

Comprehensive Swelling Analysis of Alginate Hydrogel

Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7

Acidity Constant Estimation of Weakly Acidic Polyelectrolyte by Linear Approximation: A Case Study for Polyphosphate via Gran’s Approach

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Determining the Structure of Hexametaphosphate by Titration and 31P-NMR Spectroscopy

Cited by 7 publications

References 29 publications

Comprehensive Swelling Analysis of Alginate Hydrogel

Comprehensive Swelling Analysis of Alginate Hydrogel

Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7

Acidity Constant Estimation of Weakly Acidic Polyelectrolyte by Linear Approximation: A Case Study for Polyphosphate via Gran’s Approach

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Product

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Determining the Structure of Hexametaphosphate by Titration and ³¹P-NMR Spectroscopy