Detonation Nanodiamonds for Rapid Detection of Clinical Isolates of Mycobacterium tuberculosis Complex in Broth Culture Media

Soo, Po-Chi; Kung, Ching-Jen; Horng, Yu-Tze; Chang, Kai‐Chih; Lee, Jen-Jyh; Peng, Wen-Ping

doi:10.1021/ac301767z

Cited by 30 publications

(36 citation statements)

References 33 publications

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“…These include DNA probe hybridization-and nucleic acid amplification-based assays with or without sequencing (1-4) and high-performance liquid chromatography (HPLC) to evaluate the mycolic acid composition (1,2). More recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a reliable and rapid means for the identification of bacterial species, including mycobacteria through the recognition of specific proteins (5), PCR amplification profiles (6), or protein spectral profiles (7,8). Advanced databases for use with the latter approach are currently being expanded.…”

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confidence: 99%

Rapid Inactivation of Mycobacterium and Nocardia Species before Identification Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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The identification of mycobacteria outside biocontainment facilities requires that the organisms first be rendered inactive. Exposure to 70% ethanol (EtOH) either before or after mechanical disruption was evaluated in order to establish a safe, effective, and rapid inactivation protocol that is compatible with identification of Mycobacterium and Nocardia species using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). A combination of 5 min of bead beating in 70% EtOH followed by a 10-min room temperature incubation period was found to be rapidly bactericidal and provided high-quality spectra compared to spectra obtained directly from growth on solid media. The age of the culture, the stability of the refrigerated or frozen lysates, and freeze-thaw cycles did not adversely impact the quality of the spectra or the identification obtained. Different approaches have been developed to hasten the identification of clinically significant mycobacteria compared to traditional biochemical phenotyping. These include DNA probe hybridization-and nucleic acid amplification-based assays with or without sequencing (1-4) and high-performance liquid chromatography (HPLC) to evaluate the mycolic acid composition (1, 2). More recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a reliable and rapid means for the identification of bacterial species, including mycobacteria through the recognition of specific proteins (5), PCR amplification profiles (6), or protein spectral profiles (7,8). Advanced databases for use with the latter approach are currently being expanded. Regardless of which option is used, it is essential that cultures are rendered nonviable prior to processing outside appropriate biocontainment facilities for obvious safety reasons. Previous reports have identified a variety of ways in which mycobacteria, including Mycobacterium tuberculosis can be successfully inactivated prior to manipulation, including heat treatment (9), exposure to lethal irradiation with or without photosensitizing chemicals (10-12), alcohol exposure (13), or a combination of treatments (14-16). While most were successful, temperatures and/or exposure times varied in order to achieve uniform cell death. Treatment with disinfecting agents such as 2% glutaraldehyde was not considered an option as it can result in extensive cross-linking of the nucleic acids, proteins, and other cellular components that are necessary for the MALDI-TOF MS identification process. Within the past year, two publications described the successful adaptation of a mycobacterial inactivation/ extraction protocol for use with MALDI-TOF MS-based identification (17, 18). Herein, we report a detailed description of an even more convenient process that was first presented in part in 2013 (19). MATERIALS AND METHODSAll studies involving mycobacteria were performed in a biological safety level 3 (BSL-3) laboratory. For various aspects of this study, 28 strains encompass...

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confidence: 99%

Rapid Inactivation of Mycobacterium and Nocardia Species before Identification Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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“…In this manner, TB can be rapidly diagnosed with good specificity and sensitivity, albeit at high costs. [21,22] However, this study was performed in broth culture media, and the selectivity in the real complex environments was yet confirmed.…”

Section: Detonation Nanodiamond (Dnd) Detection Systemsmentioning

confidence: 82%

Nanomaterials in the Prevention, Diagnosis, and Treatment of Mycobacterium Tuberculosis Infections

Liang

Ding

et al. 2017

Adv Healthcare Materials

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worldwide was estimated at a staggering 10.4 million, with a significant percentage (10%) resulting from concomitant HIV-TB coinfections. [1] Given the closely knitted association between TB infections and poor sanitation/poverty, it thus comes with no surprise that an overwhelming majority of disease burden (60%) is actually shouldered by countries from the developing world (Figure 1). [1] TB infections are compounded by the emergence of multidrug resistance (MDR). In spite of the tremendous amount of resources and research dollars that have been devoted to TB research, TB diagnostics and treatment outcomes still remain startlingly poor. TB remains worryingly underreported, under-diagnosed and undertreated. A 4.3 million gap was reported between incident and notified TB cases, chiefly in developing countries; majority of TB infections remain latent and unreported, and only 30% of new incident TB cases were subjected to drug susceptibility testing. [1] The Xpert Mycobacterium Tuberculosis (MTB)/RIF assay remains the only available WHOrecommended rapid diagnostic platform for detection of TB and drug (rifampicin) resistance. Treatment success rates are highly variable, ranging from 28% (extensively drug-resistant strains) to 83% (garden strains) of TB. The rate of decline in TB incidence has remained stagnant at 1.5% annually over the past decade, far lagging behind the 5% projected by the WHO's End TB Strategy. Against such a backdrop, TB remains firmly entrenched among the top 10 causes of death worldwide. [2] To this end, the WHO has since 2015, adopted the End TB Strategy in an effort to stem this burgeoning epidemic. Major diagnostic and treatment gaps in TB management still remain, and these need to be addressed to effectively curtail TB spread and its impact on global health. Pathogenesis of TB InfectionsMTB is the infectious agent responsible for TB infections. It originates from the family Mycobacteriaceae and is an obligate bacteria chiefly spread via air droplets.

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“…Due to their target specificity, anti-bacterial properties and small size, they are primarily used as carriers for: chemotherapeutic anticancerous agents and anti-bacterial drugs. 14,[19][20][21] , growth factors such as bone morphogenic protein 2 (BMP 2) and fibroblast growth factor (FGF) 22 , and DNA for gene therapy 23 .…”

Section: Accepted Version Science Of Advanced Materialsmentioning

confidence: 99%

Dental Applications of Nanodiamonds

Najeeb¹,

Khurshid²,

Agwan³

et al. 2016

sci adv mater

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Nanodiamonds (NDs) have been used in various fields of medicine such as drug delivery, tissue regeneration and gene therapy. Although there has been research carried out investigated the potential of these remarkable materials in dentistry, to date no review has been published to summarize the studies conducted.Due to their target cell specificity, small size and fluorescence they have also been found to be usefulness in dentistry. Main applications of NDs in dentistry and medicine include guided tissue regeneration, reinforcement of polymers and drug delivery to treat infections and cancers. Recent research also suggests that NDs can also be used as bioactive or antibacterial dental implant coatings. However, to date, the research conducted on their biocompatibility is limited and inconclusive. Hence, substantially more in vitro and in vivo studies are required to envisage the future of NDs in dentistry. It is hoped that in the next decade these promising materials will find a variety of uses in routine dentistry.

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Detonation Nanodiamonds for Rapid Detection of Clinical Isolates of Mycobacterium tuberculosis Complex in Broth Culture Media

Cited by 30 publications

References 33 publications

Rapid Inactivation of Mycobacterium and Nocardia Species before Identification Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Rapid Inactivation of Mycobacterium and Nocardia Species before Identification Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Nanomaterials in the Prevention, Diagnosis, and Treatment of Mycobacterium Tuberculosis Infections

Dental Applications of Nanodiamonds

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