Purpose–Job stress and presenteeism are stressors that have become chronic problems in the current business world. The primary objective of this study is to assess the levels of job stress and presenteeism among employees in five-star hotels, and to examine the potential interaction between these two factors.Design/Methodology/Approach–The survey method, classified as a descriptive model type, was chosen for the research objectives. The quantitative approach was implemented. The research was conducted utilizing the survey method and measurement instruments in accordance with this premise. The employee of five-star hotels in Istanbul that possess "Tourism Operation Certificate" comprise the research sample. 392 hotel employees participated in the research.Results–Based on the data derived from this research, it can be concluded that there is a substantial positive correlation between job stress levels experienced by hotel employees and their inclination to engage in presenteeism behavior. It can be inferred that employees who are under increased levels of job-related stress will demonstrate a higher likelihood of presenteeism, as supported by this finding. For the purpose of the research, frequency analysis, factor analysis, normality tests, correlation, and regression analyses were used.Discussion–It can be stated that businesses that do not want to face various costs for the orientation and training of new personnel should take into account situations such as job stress and presenteeism for the continuity of the business. In addition, relevant public institutions have great responsibilities to determine and supervise whether overtime is applied to personnel, whether wages are paid regularly and in accordance with the rules, whether the working environment is appropriate, and whether social rights are provided. Non-governmental organizations also have important duties in this regard