Developing an alginate/chitosan hybrid fiber scaffold for annulus fibrosus cells

Shao, Xinxin; Hunter, Christopher

doi:10.1002/jbm.a.31030

Cited by 108 publications

(105 citation statements)

References 31 publications

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“…AF tissue engineering approaches have evaluated AF cells seeded in several types of 3D hydrogels or scaffolds [7,[14][15][16][17][18]. These studies have demonstrated that AF cells proliferate in 3D culture and synthesize ECM, reconstituting the histological structure of native tissue.…”

Section: Introductionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

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Purpose Annulus fibrosus (AF) tissue engineering is gathering increasing interest for the development of strategies to reduce recurrent disc herniation (DH) rate and to increase the effectiveness of intervertebral disc regeneration strategies. This study evaluates the use of a bioactive microfibrous poly(L-lactide) scaffold releasing Transforming Growth Factor (TGF)-b1 (PLLA/TGF) for the repair and regeneration of damaged AF. Methods The scaffold was synthesized by electrospinning, with a direct incorporation of TGF-b1 into the polymeric solution, and characterized in terms of morphology and drug release profile. Biological evaluation was performed with bovine AF cells (AFCs) that were cultured on the scaffold up to 3 weeks to quantitatively assess glycosaminoglycans and total collagen production, using bare electrospun PLLA as a control. Histological evaluation was performed to determine the thickness of the deposited neo-ECM. Results Results demonstrated that AFCs cultured on PLLA/TGF deposited a significantly greater amount of glycosaminoglycans and total collagen than the control, with higher neo-ECM thickness. Conclusions PLLA/TGF scaffold induced an anabolic stimulus on AFCs, mimicking the ECM three-dimensional environment of AF tissue. This bioactive scaffold showed encouraging results that allow envisaging an application for AF tissue engineering strategies and AF repair after discectomy for the prevention of recurrent DH.

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Section: Introductionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

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“…Increasing the degree of de-acetylation prolongs its degradation time while enhancing cell adhesion (Mao et al, 2004;Di Martino et al, 2005 In vivo differentiation to NP-like cells. Bertolo et al, 2012;Cheng et al, 2010;Cheng et al, 2013;Dai et al, 2011;Iwasaki et al, 2004;Li et al, 2005b;Mao et al, 2004;Di Martino et al, 2005;Patel et al, 2010;Pusateri et al, 2003;Richardson et al, 2008;Roughley et al, 2006;Shao and Hunter, 2007;Sun et al, 2014;Zhang et al, 2014 Alginate Brown seaweed Delayed drug delivery systems Cells cultured in 3D constructs, tested in vivo in murine model. Bron et al, 2011;Chou et al, 2009;Chou and Nicoll, 2009;Guo et al, 1989;Larsen and Haug, 1971;Leone et al, 2008;…”

Section: Natural Hydrogels Chitosanmentioning

confidence: 99%

“…Bovine NP cells cultured in chitosan scaffolds produced an extracellular matrix (ECM) similar to that of the native NP, and more so than bovine AF cells (Roughley et al, 2006). Canine AF cells cultured in alginate/chitosan scaffolds formed clusters and produced collagens I, II and aggrecan (Shao and Hunter, 2007). The addition of chitosan to alginate proved to increase degradation time for the scaffold.…”

Section: Natural Solid Biomaterialsmentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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“…The three main components of IVD aimed at tissue regeneration are: 1) AF (Sato et al, 2003;Chang et al, 2007;Helen and Gough, 2007;Nerurkar et al, 2007;Shao and Hunter, 2007;Wan et al, 2007;Nerurkar et al, 2008;Wang et al, 2008;Nerurkar et al, 2009); 2) NP (Baer et al, 2001;Sakai et al, 2003;Seguin et al, 2004;Bertram et al, 2005;Wilke et al, 2006;Roughley et al, 2006;Cloyd et al, 2007;Chou et al, 2008;Halloran et al, 2008;Richardson et al, 2008;Vernengo et al, 2008;Bron et al, 2009;Chou et al, 2009;Sawamura et al, 2009;Calderon et al, 2010;Silva-Correia et al, 2010a;Silva-Correia et al, 2010b;Reza and Nicoll, 2010;); and 3) both NP and AF by means of using biphasic scaffolds (Mizuno et al, 2004;Hamilton et al, 2005;Mizuno et al, 2006;Nerurkar et al, 2008;Nesti et al, 2008). It has been proposed that for producing a tissue resembling natural IVD, total IVD replacement could be required.…”

Section: Polymeric-based Designsmentioning

confidence: 99%

“…For example, Sato et al (Sato et al, 2003) demonstrated that AF cells isolated from rats retained a fibrocartilaginous phenotype and produced more ECM in 3D cultures compared to 2D (monolayer). To obtain scaffolds with unidirectional aligned fibres, Shao et al (Shao et al, 2007) produced wet-spinned composite scaffolds of alginate and chitosan. The AF cells isolated from dogs were found to survive and proliferate.…”

Section: Polymeric-based Designsmentioning

confidence: 99%

Intervertebral Disc Regeneration

Libera¹,

Hoell

Holzhausen³

et al.

Fundamentals of Tissue Engineering and Regenerative Medicine

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Low back pain is an extremely common illness syndrome that causes patient suffering and disability and requires urgent solutions to improve the quality of life of these patients. Treatment options aimed to regenerate the intervertebral disc (IVD) are still under development. The cellular complexity of IVD, and consequently its fine regulatory system, makes it a challenge to the scientific community. Biomaterials-based therapies are the most interesting solutions to date, whereby tissue engineering and regenerative medicine (TE&RM) strategies are included. By using such strategies, i.e., combining biomaterials, cells, and biomolecules, the ultimate goal of reaching a complete integration between native and neo-tissue can be achieved. Hydrogels are promising materials for restoring IVD, mainly nucleus pulposus (NP). This study presents an overview of the use of hydrogels in acellular and cellular strategies for intervertebral disc regeneration. To better understand IVD and its functioning, this study will focus on several aspects: anatomy, pathophysiology, cellular and biomolecular performance, intrinsic healing processes, and current therapies. In addition, the application of hydrogels as NP substitutes will be addressed due to their similarities to NP mechanical properties and extracellular matrix. These hydrogels can be used in cellular strategies when combined with cells from different sources, or in acellular strategies by performing the functionalization of the hydrogels with biomolecules. In addition, a brief summary of therapies based on simple injection for primary biological repair will be examined. Finally, special emphasis will focus on reviewing original studies reporting on the use of autologous cells and biomolecules such as platelet-rich plasma and their potential clinical applications.

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Developing an alginate/chitosan hybrid fiber scaffold for annulus fibrosus cells

Cited by 108 publications

References 31 publications

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Intervertebral Disc Regeneration

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