Developing an evidence‐based guideline for the management of exposure to hepatitis B at a Victorian tertiary hospital

Pierce, Anna; Armishaw, Jude; Aitchison, Stacey

doi:10.1111/1753-6405.12107

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…An E. coli strain, Rosseta‐Gami B (DE3) 25 pLysS, was transformed with the pGEX‐5X‐3 GST control, GST‐TIMM29 wild type, 26 and mutants (M1 to M4). Protein expression was induced with 0.5 mM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG) at 30°C overnight when E. coli growth reached 0.3 of OD 600 .…”

Section: Methodsmentioning

confidence: 99%

TIMM29 interacts with hepatitis B virus preS1 to modulate the HBV life cycle

Muriungi

Ueda

2020

Microbiology and Immunology

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Hepatitis B virus (HBV), a major global health problem, can cause chronic hepatitis, liver cirrhosis, and hepatocellular carcinomas in chronically infected patients. However, before HBV infection can be adequately controlled, many mysteries about the HBV life cycle must be solved. In this study, TIMM29, an inner mitochondrial membrane protein, was identified as an interaction partner of the preS1 region of the HBV large S protein. The interaction was verified by both an immunoprecipitation with preS1 peptides and a GSTpulldown assay. Immunofluorescence studies also showed colocalization of preS1 and TIMM29. Moreover, it was determined that the preS1 bound with amino acids 92-189 of the TIMM29 protein. Infection of HBV in TIMM29overexpressing NTCP/G2 cells resulted in a significant decrease of HBeAg and both extracellular particle-associated and core particle-associated HBV DNA without affecting cccDNA formation. Comparable results were obtained with TIMM29-overexpressing HB611 cells, which constitutively produce HBV. In contrast, knockout of TIMM29 in NTCP/G2 cells led to a higher production of HBV including HBeAg expression, as did knockout of TIMM29 in HB611. Collectively, these results suggested that TIMM29 interacts with the preS1 region of the HBV large S protein and modulates HBV amplification.

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Section: Methodsmentioning

confidence: 99%