Developing an ultra‐performance liquid chromatography‐tandem mass spectrometry for detecting aldosterone in human plasma

Lin, Wenbin; Yao, Zhenrong; Li, Yuzhe; Liao, Zhiwei; Xiao, Jiahao; Chen, Yonghong; Feng, Pinning; Gan, Wenjia

doi:10.1002/jcla.24029

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…Plasma aldosterone concentrations of vertebrates can vary widely. For example, an order of magnitude variation is seen within aldosterone reference intervals for both humans and dogs, with concentrations generally ranging from the tens to low hundreds pg ml -1 (Meunier et al 2015, Eisenhofer et al 2017, Tan et al 2020, Lin et al 2021, Kai et al 2022. Initial aldosterone concentrations in Kemp's ridley turtles in this study were substantially higher than maximal values within these mammalian reference intervals, and with 1 exception were higher than previously published data for aldosterone in reptiles, including limited data for 4 Lepidochelys kempii (Bradshaw & Grenot 1976, Uva et al 1982, Balment & Loveridge 1989, Ortiz et al 2000; Table 3).…”

Section: Discussionmentioning

confidence: 99%

Endocrine data provide further evidence of physiologic derangement in sea turtles affected by the Deepwater Horizon oil spill

Innis,

Graham,

Stacy

et al. 2024

Endang. Species. Res.

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This study was conducted to characterize the adrenocortical and thyroid status of Kemp’s ridley sea turtles Lepidochelys kempii that were affected by the Deepwater Horizon oil spill. A plasma aldosterone assay was validated for L. kempii, and it was used along with previously validated assays for corticosterone and free thyroxine to assess hormone concentrations of 30 L. kempii that were hospitalized due to oil exposure, representing 2 severities of oiling (lightly or heavily oiled). Hormone concentrations were also assessed in relation to 8 clinical biochemical analytes. Analysis of paired samples indicated that oiled turtles had significantly higher initial aldosterone and corticosterone concentrations, which declined during convalescence (average 96 and 90% decrease, respectively). Thyroxine concentrations significantly increased between admission and convalescence (average 65% increase). Initial biochemical data indicated significantly higher plasma potassium, ionized magnesium, and lactate concentrations compared to convalescent values. Aldosterone concentrations were positively correlated with corticosterone, negatively correlated with free thyroxine, and variably correlated with several clinical biochemical analytes. Results of this study indicate that L. kempii had robust adrenocortical activity after oiling, capture, and transport to the hospital, regardless of the degree of oiling, resulting in very high plasma concentrations of aldosterone and corticosterone. This study also confirms that aldosterone can be reliably measured in sea turtle plasma samples, providing another diagnostic tool for the physiologic assessment of this Critically Endangered species.

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