A multi-year analysis of the state of the livestock sector in the period before the outbreak of war in 2022 is important for the post-war recovery of Ukraine’s regions. The purpose of the research was to identify the factors of losses in the production of livestock products, methods and means of integrated development. The generally accepted methods in zootechnology were followed, the study was conducted over a thirty-year period (1991-2021) in accordance with the stages of research work number: 0121U113933 of 18.11.2021. It was found that the number of cattle decreased by 8.4 times, and cows – by 5.2 times. Gross milk production decreased by 2.7 times, and beef and veal production – by 4.5 times. Improving the genetic potential of livestock, feeding, and housing contributed to an increase in cow productivity by 2,842 kg on average in the region and by 4,693 kg at the enterprises. As of 01.01.2021, 5 stud farms of the Ukrainian Black-and-White dairy breed were established with a population of 12,369 heads, including 4,647 cows, with a milk yield of 9,749 kg in 2020. The profitability of milk production in 2019 was 20.6%, while cattle breeding for meat was unprofitable (-27.1%). Many farms turn manure into compost and apply it to the soil for ploughing. Between 1991 and 2021, the Kharkiv region lost the potential of the dairy industry, the number of cattle decreased, with the largest decline in 1991-2001 (6.54 times). Milk production decreased by 2.7 times and beef and veal production by 4.5 times, with the main “drop” occurring between 1990 and 2010 (by 2.7 and 4.5 times respectively). The average annual milk yield per cow in the region from 1990 to 2020 increased by 2,840 kg and in 2020 amounted to 5,821 kg. Practical value: the article is the first to provide a long-term analysis of the state of the cattle breeding industry before the outbreak of hostilities in Ukraine, taking into account technological, organisational and economic aspects