Developing elite <i>Neurospora crassa</i> strains for cellulosic ethanol production using fungal breeding

Waters, Joshua C.; Nixon, Andrew B.; Dwyer, Morgan; Biffinger, Justin C.; Lee, Kwangwon

doi:10.1007/s10295-017-1941-0

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…These enzymes are used in various applications; xylanases are applied in the feed industry [58], amylases in the textile industry to separate fibers, and cellulases for fruit liquefaction [59]. In addition, PI values for Trichoderma and Neurospora generans of 0.89 and 0.8, respectively [41,60], have also been reported; these fungi have been used to produce cellulases, which are used in the beverage industry [61]. However, there are few studies on these types of microorganisms in terms of their potential to express laccase.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Núñez‐García,

Martínez‐Ávila,

González‐Herrera

et al. 2024

J Basic Microbiol

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Endophytic microbial communities colonize plants growing under various abiotic stress conditions. Candelilla (Euphorbia antisyphilitica Zucc.) is a shrub that develops functionally in arid and semi‐arid zones of Mexico; these conditions generate an association between the plant and the microorganisms, contributing to the production of enzymes as a defense mechanism for resistance to abiotic stress. The objective of this research was to isolate and identify endophyte fungi of candelilla and bioprospection of these endophytic fungi for enzyme production using candelilla by‐products. Fungi were isolated and identified using ITS1/ITS4 sequencing. Their potency index (PI) was evaluated in producing endoglucanase, xylanase, amylase, and laccase. Fermentation was carried out at 30°C for 8 days at 200 rpm, with measurements every 2 days, using candelilla by‐products as substrate. All fungi exhibited higher cellulase, amylase, and laccase activities on the 2nd, 6th, and 8th day of fermentation, respectively, of fermentation. The fungus Aspergillus niger ITD‐IN4.1 showed the highest amylase activity (246.84 U/mg), the genus Neurospora showed the highest cellulase activity, reaching up to 13.45 FPU/mg, and the strain Neurospora sp. ITD‐IN5.2 showed the highest laccase activity (3.46 U/mg). This work provides the first report on the endophytic diversity of E. antisyphilitica and its potential role in enzyme production.

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Section: Discussionmentioning

confidence: 99%

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Núñez‐García,

Martínez‐Ávila,

González‐Herrera

et al. 2024

J Basic Microbiol

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“…Trichoderma viride can produce large amounts of endoglucanase, while A. niger produces a cellulose enzyme containing β-glucosidase, yet low in exo and endoglucanase [21]. In addition to T. viride and A. niger, N. sitophila has high cellulolytic and hemicellulose activities in fermentation to produce simple sugars [22]. N. sitophila is strong amylolytic by α-amylase and glucoamylase enzymes production, yet inadequate in its lipolytic and proteolytic properties, while Rhizopus oryzae produce carbohydrate degrading enzymes such as amylase, cellulase, xylanase, and glucoamylase [23].…”

Section: Introductionmentioning

confidence: 99%

Degradation of Lignocelluloses Cocoa Shell (Theobroma cacao L.) by Various Types of Mould Treatments

Djali

Kayaputri

Kurniati

et al. 2021

Journal of Food Quality

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Lignocellulose can be degraded by lignocellulolytic microorganisms such as moulds. The purpose of the study was to obtain the right type of moulds in degrading lignocellulose on the cocoa shell powder. The study used a completely randomized design method using four treatments of different types of mould (Trichoderma viride, Neurospora sitophila, Aspergillus niger, and Rhizopus oryzae) towards cocoa shell powder fermentation. Solid fermentation of cocoa shell powder was carried out for 5 days in an incubator with a temperature of 30°C for T. viride, N. sitophila, and R. oryzae, while A. niger of 35°C. The fermented substrate was then dried in a cabinet oven with a temperature of 50°C for 4 days. Tests of lignin, cellulose, and hemicellulose were performed towards the treatments by the Chesson method, while the moisture content test was performed using the AOAC method. Degradation of fermented cocoa shell powder has shown a significant effect on moisture, lignin, cellulose, and hemicellulose contents. Trichoderma viride resulted in the highest lignocellulose degradation compared with the other treatments. The percentage decrease of lignin content is up to 46.69 wt%; while cellulose of 22.59 wt%; and hemicellulose is about 19.41 wt% from the initial lignin weight.

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“…There is also a growing concern that genetic manipulations that are effective in laboratory strains may not be translatable to more robust strains used in industry [3,5]. There are, however, organisms that already possess the genetic elements needed to perform all of the requisite processes, such as the model filamentous fungus Neurospora crassa [9].…”

Section: Introductionmentioning

confidence: 99%

“…Selective mating has long been used in plant breeding to improve traits ranging from fruit size and yield to appearance or flavor [7,8]. Recently, selective breeding has also been used in fungi in attempts to select for increased saccharification and fermentation potential [9].…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci (QTL) underlying phenotypic variation in bioethanol-related processes in Neurospora crassa

et al. 2020

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Bioethanol production from lignocellulosic biomass has received increasing attention over the past decade. Many attempts have been made to reduce the cost of bioethanol production by combining the separate steps of the process into a single-step process known as consolidated bioprocessing. This requires identification of organisms that can efficiently decompose lignocellulose to simple sugars and ferment the pentose and hexose sugars liberated to ethanol. There have been many attempts in engineering laboratory strains by adding new genes or modifying genes to expand the capacity of an industrial microorganism. There has been less attention in improving bioethanol-related processes utilizing natural variation existing in the natural ecotypes. In this study, we sought to identify genomic loci contributing to variation in saccharification of cellulose and fermentation of glucose in the fermenting cellulolytic fungus Neurospora crassa through quantitative trait loci (QTL) analysis. We identified one major QTL contributing to fermentation of glucose and multiple putative QTL's underlying saccharification. Understanding the natural variation of the major QTL gene would provide new insights in developing industrial microbes for bioethanol production.

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Developing elite Neurospora crassa strains for cellulosic ethanol production using fungal breeding

Cited by 7 publications

References 39 publications

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphiliticaZucc): Potential for extracellular enzyme production

Degradation of Lignocelluloses Cocoa Shell (Theobroma cacao L.) by Various Types of Mould Treatments

Quantitative trait loci (QTL) underlying phenotypic variation in bioethanol-related processes in Neurospora crassa

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