Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

Zhou, Qi; Bi, Zhuangli; Yin, Daqiang; Gu, Xiangxue; Xu, Zejun; Huang, Rong; Xue, Xing; Qi, Kezong; Wang, Guijun

doi:10.1007/s00705-019-04495-4

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…Up to now, extensive and in-depth researches about pathogen (5,23,24), pathogenicity (6,25,26), epidemiology (5,27), rapid diagnosis (28)(29)(30), and vaccine (31) were carried out to prevent and control DTMUV infection. Recently, we revealed the expression profile and biological function of mRNAs in DTMUV-infected DEFs (8).…”

Section: Introductionmentioning

confidence: 99%

Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus

Lin

Yang

et al. 2020

Front. Immunol.

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Duck Tembusu virus (DTMUV), the causative agent of egg-drop syndrome, has caused substantial economic losses to duck industry. DTMUV infection leads to profound changes of host cells, including transcriptome and proteome. However, the lncRNA expression profile and the biological function of lncRNA have not been revealed. Therefore, DTMUV was used to inoculate duck embryo fibroblast cells (DEFs) for high-throughput RNA-sequencing (RNA-Seq). The results showed that 34 and 339 differently expressed lncRNAs were, respectively, identified at 12 and 24 h post-infection (hpi). To analyze their biological functions, target genes in cis were searched and the regulatory network was formed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes were strongly associated with immune system, signaling molecular and interaction, endocrine system, and signal transduction. The differently expressed lncRNAs were selected and verified by quantitative real-time polymerase chain reaction (RT-qPCR). Our study, for the first time, analyzed a comprehensive lncRNA expression profile in DEFs following DTMUV infection. The analysis provided a view on the important roles of lncRNAs in gene regulation and DTMUV infection.

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Section: Introductionmentioning

confidence: 99%

Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus

Lin

Yang

et al. 2020

Front. Immunol.

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“…Histopathological results showed (Figure . 7) that hepatic cell steatosis, lymphocyte in ltration and necrosis, and liver haemorrhage were observed in duck liver infected with GZSS2022 (Zhou et al, 2019). GZSS2022 infected ducks with brain congestion, and lymphocytes and monocytes exuded from blood gathered around blood vessels to form vascular sheaths (Yao et al, 2019).…”

Section: Pathological Changes In Ducklings Arti Cially Infected With ...mentioning

confidence: 99%

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

Luo

Long

et al. 2023

Preprint

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Since 2010, several strains of Duck Tembusu Virus (DTMUV) have been isolated from China. In autumn 2021, high mortality was observed in southwestern Guizhou Province, China, and a DTMUV strain named GZSS2022 was successfully isolated. After inoculation with SPF chicken embryos of 10 days age, the embryos died 3-5 days after inoculation, and RT-PCR could still detect DTMUV positive after collecting the allantoic fluid of dead chicken embryos and transmitting it for 4 generations. In addition, the GZSS2022 strain was able to replicate in BHK-21 cells and induce cytopathic effects (CPE), and the virus particles were observed in the cytoplasm by transmission electron microscopy. In animal experiments, GZSS2022 strain can replicate stably and pathological examination results showed that the brain of infected ducks was congested and vascular sheath formed around the blood vessels; Liver lymphocyte infiltration, necrosis, liver bleeding. The E gene of this strain was sequenced by PCR and its homology and genetic evolution were analyzed. It was found that the homology of GZSS2022 with the Beijing duck reference strain (MT108699.1) was the highest 99.39%, and the homology of GZSS2022 with the early isolated reference strain (FX2010) was 96.88%. The GZSS2020 strain was located in the same subbranch as the reference strain from Beijing duck in China. The results of this study indicate that a novel highly virulent Tambusu virus has appeared for the first time in Guizhou Province, China, and further lay the foundation for the study of the pathogenicity and host range of this novel Tambusu virus.

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“…Nowadays, common methods for diagnosis of TMUV include serological detection by enzyme-linked immunosorbent assay (ELISA), based on NS1 protein detection, and the plaque reduction neutralization test (PRNT) [ 81 , 82 ]. The spatial organization of the E protein has been well described in DENV, WNV and JEV, showing a high degree of similarity between flaviviruses [ 83 , 84 , 85 ].…”

Section: Clinical Features and Pathogenicitymentioning

confidence: 99%

New Insights into the Biology of the Emerging Tembusu Virus

et al. 2021

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Reported for the first time in 1955 in Malaysia, Tembusu virus (TMUV) remained, for a long time, in the shadow of flaviviruses with human health importance such as dengue virus or Japanese encephalitis virus. However, since 2010 and the first large epidemic in duck farms in China, the threat of its emergence on a large scale in Asia or even its spillover into the human population is becoming more and more significant. This review aims to report current knowledge on TMUV from viral particle organization to the development of specific vaccines and therapeutics, with a particular focus on host–virus interactions.

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Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

Cited by 6 publications

References 29 publications

Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus

Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

New Insights into the Biology of the Emerging Tembusu Virus

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