Development and Application of Devices Based on Surface Plasmon Resonance

Abstract

Introduction. The purpose of the SPR sensor is to quickly and accurately determine the refractive index of the environment with the ability to diagnose the presence of a specific substance. SPR devices and biosensor diagnostic methods for laboratory diagnostics in medicine, veterinary medicine, determination of environmental pollution, for food quality control are being developed. The work is devoted to the development of devices based on the surface plasmon resonance of the “Plasmontest” series, which can be used for refractometric and biosensor applications. The purpose of the paper is to present the development of “Plasmontest” series devices that can be used for laboratory applications, as portable field-research devices and to carry out a comparison of optical circuits of SPR devices, their capabilities and operational characteristics during biochemical and physical experiment. Results. Specific features of the design of devices "Plasmontest" with discrete and aperture optical circuits are outlined. The method of approximation of the resonant SPR curve is proposed to accurately find the value of the resonance minimum. Procedures for normalizing and calibration of devices with aperture optical circuit in single-channel and dual-channel design are developed to improve measurement accuracy. Some features of the developed software for “Plasmontest” series devices are presented. Applications of "Plasmontest" devices for the refractometry and the development of thin-film technological processes as well as for creating the methods of immunosensory detection of a number of bacteria and toxins are described. Conclusions. The work on the creation of “Plasmontest” series devices showed the possibility for creating portable SPR devices and for refractometric, thin film and biosensor studies. It is shown that devices with aperture optical circuitry are the most promising in terms of compactness, reliability and low cost.

Application of the "Plazmontest" Software and Hardware Complex for the Determination of Spermine Concentration

“…Особливість приладу -можливість його використання зі стаціонарним комп'ютером, ноутбуком, а також в автономному режимі, з відображенням ППР-кривих, сенсограм або результатів діагностики на дисплеї приладу [6].…”

“…Наступний етап -побудова апроксимуючих поліномів та розрахунок точного мінімуму кривої ППР. Для цього використовується поліноміальна апроксимація 3-го порядку для 300 точок, яка враховує асиметрію ППР-кривої та дає найкраще середнє відхилення мінімуму кута [6].…”

Identification Antigens of Different Nature Using Immunobiosenors on a Single Platform Based on Surface Plasmon Resonance

“…These biosensors detect changes in the dielectric constant in the area of the gold chip of the sensor caused by the formation or dissociation of the binding of analytes with ligands attached to the surfaces, which causes a shift in the resonance angle [2]. Domestic authors have developed and manufactured a series of devices under the general name Plazmontest, which are used in refractometric and biochemical studies [3]. Methods for the detection of some mycotoxins [4] were developed with the help of the Plazmontest device, and the device was also used with efficiency to develop methods for the detection of Salmonella typhimurium [5].…”