Development and Application of Immunoaffinity Column for the Simultaneous Determination of Norfloxacin, Pefloxacin, Lomefloxacin, and Enrofloxacin in Swine and Chicken Meat Samples

Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples

“…To assess the prepared immunoaffinity column, pork meat obtained from local supermarket was fortified with ENR at the final concentration of 10, 25 and 50 ng/g. A total of 5 g homogenized muscle tissues were mixed with 5 mL 0.01 mol/L PBS (pH 7.4) in polypropylene centrifuge tubes followed by vortexing for 5 min (Sun et al, 2016). After centrifugation at 9000 rpm for 15 min, the supernatants were collected, and the precipitate was pre-extracted with 5 mL 0.01 mol/L PBS (pH 7.4).…”

“…The selection of the most appropriate eluting conditions was made considering (1) the recovery of the analyte, (2) the volume needed for an acceptable recovery, (3) the potential damage of the IAC after several cycles of usage and (4) the compatibility with the immunochemical analytical method (Sanvicens, Moore, Guilbault, & Marco, 2006). Previous research has shown that the mixtures of organic solvents, such as methanol or acetonitrile, were suitable to remove analytes from IAC (Sun et al, 2016;Wang et al, 2011). In this study, five eluting solutions with different methanol concentrations were evaluated.…”

“…Immunoaffinity chromatography (IAC) is a powerful method for sample clean-up, which enables selective extraction and concentration of individual compounds or classes of compounds from complex matrices in only one step (Şenyuva & Gilbert, 2010). The application of IAC for a variety of samples pretreatment in mycotoxins (Lattanzio, Ciasca, Powers, & Visconti, 2014;Pascale, Panzarini, Powers, & Visconti, 2014;Pavšič-Vrtač, Ojanperä, Apajalahti, Šrimpf, & Tavčar-Kalcher, 2014), veterinary drugs (Sun et al, 2016;Wang, Xu, Zhang, Wang, & Dong, 2011;Zhao, Lin, Song, Pan, &pesticides (Esteve-Turrillas, Mercader, Agullo, Abad-Somovilla, &Abad-Fuentes, 2011;Xu et al, 2012) residues analyses have been reported. In this work, we describe the generation and application of polyclonal antibody (pAb)-based IAC as a clean-up procedure, followed by the enzyme-linked immunosorbent assay (ELISA) analysis of ENR in real food samples.…”

Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

“…Ommunoaffinity chromatography takes the advantage of specific and reversible interaction between antibody and the analyte, and enables selective extraction and enrichment of individual compounds or classes of compounds in one step (Şenyuva & Gilbert, 2010;Pichon & Combes, 2016). On the last decade, the application of immunoaffinity chromatography for a variety of samples pretreatment in mycotoxins (Longobardi et al, 2013;Marley et al, 2015;Wilcox et al, 2015;Zhang et al, 2016), veterinary drugs (Mei et al, 2014;Sun et al, 2016;Yang et al, 2016) and pesticides (Esteve-Turrillas et al, 2011;Xu et al, 2012) residues analyses have been reported.…”

Determination of Quinolones in Human Urine and Water Samples by Ultrasound Assisted Dispersive Liquid-Liquid Microextraction Based on Solidification of Floating Organic Droplet Followed High Performance Liquid Chromatography

“…They are effective to control a wide range of bacteria, both gram positive and negative as well as other bacteria and often used in the treatment of a range of illnesses. Due to the fact that its resistance in the environment can cause undesirable effects on aquatic ecosystems and human health, QNs have obtained growing attention [1,2]. Therefore, the analysis of the occurrence, distribution and risks of quinolone antibiotics became crucial to environment monitoring.…”