Development and application of immunocytochemical staining techniques: A review

Myers, J.

doi:10.1002/dc.2840050317

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…Immunocytochemical staining for CD3 (monoclonal mouse anti‐human antibody, clone F7.2.38, DAKO, Denmark) and CD79a (monoclonal mouse anti‐human antibody, clone HM57, DAKO, Denmark) was performed . These antibodies have been demonstrated to cross‐react with the equivalent equine antigens .…”

Section: Case Presentationmentioning

confidence: 99%

Leukemic small cell lymphoma or chronic lymphocytic leukemia in a horse

Cian

Tyner

Martini

et al. 2013

Veterinary Clinical Pathol

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A 16-year-old, Irish Draft mare was admitted to the referring veterinarian for an annual health check. A mild generalized lymphadenomegaly was noted. Rectal palpation and transrectal ultrasonographic examination revealed prominent mesenteric lymph nodes. A transcutaneous abdominal ultrasonographic evaluation was unremarkable. A CBC revealed a marked leukocytosis (63.06 × 10(3)/μL) and lymphocytosis (58.2 × 10(3)/μL) due to increased numbers of small lymphocytes. No evidence of anemia or thrombocytopenia was found and neutrophil counts were low-normal. Cytologic examination of fine-needle aspirates of multiple lymph nodes and a bone-marrow aspirate revealed the presence of a monomorphic population of small lymphocytes similar to those observed in the peripheral blood, suggesting a leukemic small cell lymphoma (SCL) or chronic lymphocytic leukemia (CLL). As the lymphadenomegaly and peripheral blood lymphocytosis were present simultaneously, the distinction between these 2 conditions was not possible. Immunophenotyping by immunocytochemistry and flow cytometry of the lymphoid cells in peripheral blood determined a T-cell phenotype. As the horse was clinically stable, no treatment was initiated, but regular examinations were undertaken. A CBC repeated 120 days after the diagnosis showed a marked lymphocytosis (157.6 × 10(3)/μL) with no evidence of anemia or other cytopenias. The horse was euthanized 194 days after the initial diagnosis. Histopathology and immunohistochemistry of submandibular lymph nodes and bone marrow confirmed the diagnosis of leukemic SCL or CLL, and a T-cell phenotype. SCL and CLL are rare in horses; previous immunohistochemical studies determined that the T-cell phenotype is predominant. To the authors' knowledge, this is the first report of the combined use of immunocytochemistry and flow cytometry in a horse with leukemic SCL or CLL.

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Section: Case Presentationmentioning

confidence: 99%

Leukemic small cell lymphoma or chronic lymphocytic leukemia in a horse

Cian

Tyner

Martini

et al. 2013

Veterinary Clinical Pathol

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“…Identification of the molecular complexes is crucial to our understanding of signal transduction pathways related to disease progression (Kolch and Pitt, 2010;Papin et al, 2005). Various methods are available to detect protein-protein and protein-nucleic acid interactions, but few are able to analyze individual complexes (Auerbach et al, 2002;Cheng et al, 2004;Collas, 2009;Gavin and Superti-Furga, 2003;Kuo and Allis, 1999;Myers, 1989;Phizicky and Fields, 1995;Stenman et al, 1993;Toby and Golemis, 2001;Williams, 2000). For instance, while conventional immunoprecipitation (IP), which requires a large amount of sample, is currently the standard method for detecting protein-protein interactions, this approach cannot directly detect individual biomolecule complexes or provide an absolute number of complexes present (Jain et al, 2011;Liu et al, 2011;Thompson et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Individual Signaling Complexes by mMAPS, a Flow‐Proteometric System

Chou

Tsou

Hsu

et al. 2016

CP Molecular Biology

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Signal transduction is essential for maintaining cells’ normal physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for Multi-parameter Analysis of Proteins in a Single-complex or mMAPS for analyzing and quantifying target signaling complexes individually. mMAPS is a flow-proteometric-based system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissue samples or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe, requiring very low sample input.

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“…Cytospin preparations were also prepared on poly-L-lysine-coated slides and immunocytochemistry was performed with a monoclonal antibody against the FCoV nucleocapsid protein (mouse monoclonal, clone FIPV3-70; Novus Biologicals) using an avidin-biotin-peroxidase complex technique. 1 Negative controls were run concurrently. Thirty percent of CSF macrophages showed positive staining consistent with the presence of intracytoplasmic FCoV antigen within these cells ( Figure 2).…”

mentioning

confidence: 99%

Immunocytochemical demonstration of feline infectious peritonitis virus within cerebrospinal fluid macrophages

Ives

Vanhaesebrouck

Cian

2013

Journal of Feline Medicine and Surgery

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A 4-month-old female entire domestic shorthair cat presented with an acute onset of blindness, tetraparesis and subsequent generalised seizure activity. Haematology and serum biochemistry demonstrated a moderate, poorly regenerative anaemia, hypoalbuminaemia and hyperglobulinaemia with a low albumin:globulin ratio. Serology for feline coronavirus antibody was positive with an elevated alpha-1 acid glycoprotein. Analysis of cisternal cerebrospinal fluid (CSF) demonstrated markedly elevated protein and a mixed, predominately neutrophilic pleocytosis. Immunocytochemistry for feline coronavirus was performed on the CSF, with positive staining observed inside macrophages. The cat was subsequently euthanased, and both histopathology and immunohistochemistry were consistent with a diagnosis of feline infectious peritonitis. This is the first reported use of immunocytochemistry for detection of feline coronavirus within CSF macrophages. If this test proves highly specific, as for identification of feline coronavirus within tissue or effusion macrophages, it would be strongly supportive of an ante-mortem diagnosis of feline infectious peritonitis in cats with central nervous system involvement without the need for biopsy.

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Development and application of immunocytochemical staining techniques: A review

Cited by 9 publications

References 44 publications

Leukemic small cell lymphoma or chronic lymphocytic leukemia in a horse

Leukemic small cell lymphoma or chronic lymphocytic leukemia in a horse

Analysis of Individual Signaling Complexes by mMAPS, a Flow‐Proteometric System

Immunocytochemical demonstration of feline infectious peritonitis virus within cerebrospinal fluid macrophages

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