Development and applications of liquid chromatography-mass spectrometry for simultaneous analysis of anti-malarial drugs in pharmaceutical formulations

Chutvirasakul, Boonta; Joseph, Jan Felix; Parr, Maria Kristina; Suntornsuk, Leena

doi:10.1016/j.jpba.2020.113855

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…For example, HPLC with UV detection [ 10 ] and tandem mass spectrometry (MS) [ 11 ] were proposed for the assays of PQ and DHA in plasma. HPLC-MS was also developed for the quantitation of both drugs in combined tablets [ 12 ]. In another work, HPLC was proposed for analyses of PQ and DHA in bulk, but applications in pharmaceutical formulations were not demonstrated [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets

Pruksapha,

Khongkaew,

Suwanvecho

et al. 2023

Journal of Food and Drug Analysis

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Combination of piperaquine (PQ) (320mg) and dihydroartemisinin (DHA) (40 mg) is an anti-malarial formulation, which is recommended by World Health Organization (WHO). Simultaneous analysis of PQ and DHA can be problematic due to the lack of chromophores or fluorophores in DHA molecule. Whereas PQ possesses strong UV absorption and it presents in 8 times of DHA contents in the formulation. In this study, two spectroscopic methods, Fourier transform infrared (FTIR) and Raman spectroscopy, were developed for the determination of both drugs in combined tablets. The FTIR and Raman spectra were recorded in the attenuate total reflectance (ATR) and scattering modes, respectively. The original and pretreated spectra from FTIR and handheld-Raman were subjected to Unscrambler® program to construct partial least squares regression (PLSR) model comparing with references values obtained from high performance liquid chromatography (HPLC)-UV method. The optimal PLSR models of PQ and DHA from FTIR spectroscopy were obtained from orthogonal signal correction (OSC) pretreatment at the wavenumbers 400—1,800 cm −1 and 1,400—4,000 cm −1 , respectively. For Raman spectroscopy of PQ and DHA, the optimal PLSR models were obtained from standard normal variate (SNV) pretreatment at the wavenumbers 1,200—2,300 cm −1 and OSC pretreatment at the wavenumber 400—2,300 cm −1 , respectively. Determination of PQ and DHA in tablets from the optimum model was compared with HPLC-UV method. Results were not significantly different at 95% confidence limit ( p -value >0.05). The chemometrics-assisted spectroscopic methods were fast (1–3 min), economical and less labor intensive. Moreover, the handheld Raman spectrometer is portable and can be utilized for onsite analysis to facilitate the detection of counterfeit or substandard drugs at ports of entry.

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Section: Introductionmentioning

confidence: 99%

Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets

Pruksapha,

Khongkaew,

Suwanvecho

et al. 2023

Journal of Food and Drug Analysis

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“…ARTS decomposition was studied in methanol, methanol and water (90:10 v/v), and methanol and ammonium acetate (85:15 v/v). These are the common solvents used in chromatographic analysis [33][34][35]. The decomposition products as well as the rates of decomposition were compared in three solvent systems.…”

Section: Introductionmentioning

confidence: 99%

LC-MS/TOF Characterization and Stability Study of Artesunate in Different Solvent Systems

Oke

2022

Separations

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Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in many parts of the world. Artesunate (ARTS) is a semi-synthetic derivative of ART with improved pharmacokinetic properties. However, the half-life of ARTS is less than an hour in vivo. The analysis of this drug in vitro in different solvent systems using LC-MS/TOF showed a solvent-driven breakdown. ARTS breakdown formed several derivatives, including dihydroartemisinin (DHA), artemether (ARTM) and DHA-dimer among others, at different rates in different solvent composition systems. The change in temperature from room temperature to physiological temperature (37 °C) was found to enhance the rate of the ARTS breakdown. In methanol, ARTS mainly formed ARTM with a chromatographic peak decrease of about 3.13%, while methanol and water (90:10) v/v mainly gave rise to DHA and ARTM with about an 80% chromatographic peak decrease. On the other hand, ARTS in methanol and ammonium acetate (85:15) v/v formed DHA, ARTM, DHA-dimer and other reaction peaks with about a 97% peak decrease and the formation of an orange solution pointing to a molecular re-arrangement reaction. These results have an important bearing on research on the analysis of artemisinin drugs conducted on these common solvents.

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Optimization of HPLC–MS/MS method for determination of antimalarial adulterants in herbal products

et al. 2023

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Development and applications of liquid chromatography-mass spectrometry for simultaneous analysis of anti-malarial drugs in pharmaceutical formulations

Cited by 6 publications

References 23 publications

Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets

Chemometrics-assisted spectroscopic methods for rapid analysis of combined anti-malarial tablets

LC-MS/TOF Characterization and Stability Study of Artesunate in Different Solvent Systems

Optimization of HPLC–MS/MS method for determination of antimalarial adulterants in herbal products

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