Development and characterisation of switchable polyaniline-functionalised flow-through capillary monoliths

Abstract

Polymer monoliths were prepared in capillary format (250 mm i.d.) and used as solid supports for the immobilisation of the conducting polymer polyaniline (PANI). The immobilisation of PANI was confirmed on the large macro-porous structure of a polystyrene-divinylbenzene (PS-co-DVB) monolith. The surface coverage of polyaniline was characterised by field emission scanning electron microscopy (FE-SEM) and by capacitively coupled contactless conductivity detection (C 4 D), which was operated in scanning mode to non-invasively visualise the axial distribution of the immobilised PANI and to provide information on its doping state. To further demonstrate the successful functionalisation of the monoliths, the PANI-functionalised monoliths were demonstrated as switchable, weak anion-exchange stationary phases as confirmed by studying the retention of iodide using a perchlorate eluent.

Rapid concentration and elution of malarial antigen histidine-rich protein II using solid phase Zn(II) resin in a simple flow-through pipette tip format

“…Such options include paper monoliths formed with capillary valves or functionalized nitrocellulose. 28,29 These materials can conceivably optimize antigen delivery to RDTs impacting their performance. Another direction we would like to pursue involves the modification of the pipette requirement, either by incorporation of the flow-through column into a disposable unit or through development of a modular component that can produce the cycling vacuum needed to process each tip.…”

Contactless conductivity detection for analytical techniques— Developments from 2014 to 2016

“…C 4 D was repeatedly used for structure characterization of monolithic and open‐tubular columns for capillary chromatographic methods. Connolly and coworkers immobilized polyaniline, a conductive polymer, on a polystyrene‐divinylbenzene monolith and confirmed its immobilization by scanning C 4 D of the entire monolith. In this procedure, the detector is moved in discrete steps along the length of the column for repeated measurements.…”