Development and characterization of 11 microsatellite loci for the Mona Island iguana (Cyclura cornuta stejnegeri)

Rosas, Keysa G.; Pérez-Buitrago, Néstor; Acevedo, Jenny; Martínez, Nadia; Funk, Stephan M.; McMillan, W. Owen

doi:10.1111/j.1471-8286.2007.02080.x

Cited by 4 publications

(6 citation statements)

References 8 publications

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“…Noncoding microsatellites were used to infer patterns of population structure and rates of gene flow. Over seventy microsatellite markers were screened and characterized for this species with the 2012 samples of hatchlings and adults (all microsatellite markers designed by Rosas et al, , Welch et al, , Junghwa et al, , and Lau et al, were included in the screening). Amplifications were conducted with a 2,720 Thermal Cycler (AB Applied Biosystems) following standardized 3‐primer PCR amplification according to Schuelke () and modified by Welch et al () in a total volume of 10 µl (i.e., 7.3 µl of ddH 2 O, 1.2 µl of master mix (10 µM of each dNTPs, 10x Tricine Taq Buffer and ddH 2 O), 0.04 µl forward primer, 0.2µl reverse primer, 0.2 µl fluorescent tagged primer, 0.4 U Taq polymerase, and 1.0 µl of DNA template).…”

Section: Methodsmentioning

confidence: 99%

“…Blood was drawn from the caudal vein of adults, and tissue samples were collected from adult females that were killed by feral dogs. Blood was stored in the field at ambient Rosas et al, 2008, Welch et al, 2011, Junghwa et al, 2004, and Lau et al, 2009 were included in the screening). Amplifications were conducted with a 2,720 Thermal Cycler (AB Applied Biosystems) following standardized 3-primer PCR amplification according to Schuelke (2000) and modified by Welch et al (2011) Because mtDNA is strictly maternally inherited, an attempt was made to develop mtDNA markers to investigate sex-specific patterns of dispersal.…”

Section: Samplingmentioning

confidence: 99%

“…Samples were genotyped at 14 microsatellite loci found to be variable for C. ricordii. The polymorphic markers used were as follows: Ccste05 (Rosas et al, 2008); CIDK135, CIDK144, and CIDK184 (Welch et al, 2011); Z106, Z148, Z154, and Z494 (Junghwa et al, 2004); and D1, D11 D101, D110, D111, and D140 (Lau et al, 2009). CIDK135 locus (Welch et al, 2011) was eliminated from further analysis due to high null allele frequency p = 0.48 (Dakin & Avise, 2004; Table 1).…”

Section: Microsatellite Datamentioning

confidence: 99%

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Genetic structure at three spatial scales is consistent with limited philopatry in Ricord's Rock Iguanas (Cyclura ricordii)

León

Pasachnik

Gerber

et al. 2019

Ecology and Evolution

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Cyclura ricordii is an endemic iguana from Hispaniola Island and is threatened on the IUCN Red List. The main threats are predation by introduced mammals, habitat destruction, and hunting pressure. The present study focused on two nesting sites from Pedernales Province in the Dominican Republic. The hypothesis that natal philopatry influences dispersal and nest‐site selection was tested. Monitoring and sampling took place in 2012 and 2013. Polymorphic markers were used to evaluate whether natal philopatry limits dispersal at multiple spatial scales. Ripley's K revealed that nests were significantly clustered at multiple scales, when both nesting sites were considered and within each nesting site. This suggests a patchy, nonrandom distribution of nests within nest sites. Hierarchical AMOVA revealed that nest‐site aggregations did not explain a significant portion of genetic variation within nesting sites. However, a small but positive correlation between geographic and genetic distance was detected using a Mantel's test. Hence, the relationship between geographic distance and genetic distance among hatchlings within nest sites, while detectable, was not strong enough to have a marked effect on fine‐scale genetic structure. Spatial and genetic data combined determined that the nesting sites included nesting females from multiple locations, and the hypothesis of “natal philopatry” was not supported because females nesting in the same cluster were no more closely related to each other than to other females from the same nesting site. These findings imply that nesting aggregations are more likely associated with cryptic habitat variables contributing to optimal nesting conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Samplingmentioning

confidence: 99%

Section: Microsatellite Datamentioning

confidence: 99%

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Genetic structure at three spatial scales is consistent with limited philopatry in Ricord's Rock Iguanas (Cyclura ricordii)

León

Pasachnik

Gerber

et al. 2019

Ecology and Evolution

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“…However, the choice of future release sites should not impinge on the current natural genetic structure. Dispersal of Mona iguana hatchlings can involve longdistance movements (the maximum recorded movement is half of the diameter of Mona; Pérez-Buitrago & Sabat 2007) that may preclude any genetic differentiation among Mona localities, but this requires confirmation using molecular genetic tools (Rosas et al 2008). Second, the study highlights the gaps in our understanding of natal imprinting and homing in rock iguanas.…”

Section: Management Implicationsmentioning

confidence: 95%

Do headstart programs work? Survival and body condition in headstarted Mona Island iguanas Cyclura cornuta stejnegeri

Pérez-Buitrago¹,

Ma²,

Sabat³

et al. 2008

Endang. Species Res.

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Headstarting refers to the raising of hatchlings in captivity and subsequently releasing them into the wild and it is a widely used tool to protect endangered species by increasing survivorship. Evaluating the success of headstart programs is necessary but challenging. We evaluated a headstart program for the endangered Mona Island iguana Cyclura cornuta stejnegeri by analyzing demographic and spatial data and by comparing growth rate, body condition, and external parasitic load of headstarted and wild iguanas. Hatchlings were released at about 3 yr age. Minimum survival rate was 40.3 %, at 7.6 to 48.3 mo after release. Most migrated from the release site back to the rearing facilities about 633 m away. Density around the rearing facility increased from 7.5 to 11.5 iguanas ha-1 (25 of 75 captures were headstarted iguanas). Released headstarted iguanas exhibited growth rates similar to the maxima observed in captive and wild mid-sized iguanas. Growth rates decreased immediately prior to release, indicating that husbandry practices ought to be reviewed. There was no evidence for an effect of captivity on susceptibility to external parasites. Increased local density indicates that the program was successful. However, the longer-term success remains unknown, as most headstarted iguanas returned to the headstart facility without establishing territories typical for adult wild Mona iguanas.

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“…The hybridized fragments were then captured with streptavidin-coated magnetic Dynabeads (Dynal M-280). The enriched fragments were made double stranded by PCR with SauL-A primers (Rosas et al, 2008). PCR products were purified, ligated to Pmd18-T vector (TaKaRa) and transformed into competent cells.…”

Section: Microsatellite Locus Isolation By Magnetic Bead Methodsmentioning

confidence: 99%

Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers

Wang

Zeng²,

Xu³

2013

Genet. Mol. Res.

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ABSTRACT. The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism information content value was 0.301 to 0.830. The mean observed and expected heterozygosities varied from 0.304 to 0.460 and 0.556 to 0.621, respectively; the population from Yueqing had the smallest observed heterozygosity. In the neighbor-joining tree, Shandong, Fenghua and Yueqing populations clustered together, and there was geographic divergence between Shandong and Guangxi populations. Some microsatellite loci that were isolated from these mainland China samples were not found in blood clams collected from Malaysia.

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Development and characterization of 11 microsatellite loci for the Mona Island iguana (Cyclura cornuta stejnegeri)

Cited by 4 publications

References 8 publications

Genetic structure at three spatial scales is consistent with limited philopatry in Ricord's Rock Iguanas (Cyclura ricordii)

Genetic structure at three spatial scales is consistent with limited philopatry in Ricord's Rock Iguanas (Cyclura ricordii)

Do headstart programs work? Survival and body condition in headstarted Mona Island iguanas Cyclura cornuta stejnegeri

Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers

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