Development and Characterization of 123 SNP Markers in Neotrypaea Japonica (Ortmann 1891)

Abstract

Neotrypaea japonica (Ortmann 1891) is a burrowing shrimp that lives in tidal flats. In this study, 123 SNP markers were isolated and characterized from N. japonica. The frequency range of minor alleles varied from 0.2500 to 0.4833, the observed heterozygosity ranged from 0.4333 to 0.9000, and the expected heterozygosity ranged from 0.4944 to 0.6503. The polymorphic parameter and inbreeding value varied from 0.3680 to 0.5651 and -0.2768 to 0.1045, respectively. Significant departures from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.05) were observed for 16 loci. The study of polymorphic SNPs will provide an important theoretical basis for further population genetic diversity analysis and species conservation of N. japonica.