Development and characterization of 17 microsatellite markers for Chinese soft-shelled turtle, Pelodiscus sinensis

Ma, Xiao; Xiong, Gang; Wang, Luming; Yan, Luqi; Li, Xuejun; Xie, Dizhi; Wang, Xiaoqing

doi:10.1007/s12686-014-0253-1

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…. Recently, microsatellites were developed and characterized in this species (Que et al, 2007;Bu et al, 2011;Ma et al, 2014). The markers reported in the present study are novel and present a higher degree of polymorphism than those described previously.…”

Section: Resultsmentioning

confidence: 67%

Short Communication Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

Zhao

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26. The observed and expected heterozygosities varied from 0.208 to 0.958, and from 0.302 to 0.963, respectively. The polymorphic information content varied from 0.283 to 0.953. No significant linkage 2 T. Li et al. Genetics and Molecular Research 15 (3): gmr.15038346 disequilibrium was detected. These markers will be useful for future population genetic studies and molecular breeding of P. sinensis.

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Section: Resultsmentioning

confidence: 67%

Short Communication Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

Zhao

et al. 2016

Genet. Mol. Res.

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Millennium-old farm breeding of Chinese softshell turtles (Pelodiscus spp.) results in massive erosion of biodiversity

Gong¹,

Vamberger

Auer

et al. 2018

Sci Nat

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Chinese softshell turtles (Pelodiscus spp.) are widely distributed, ranging from the Amur and Ussuri Rivers in the Russian Far East through the Korean Peninsula, Japan, and eastern, central, and southern China to southern Vietnam. In East and Southeast Asia, Chinese softshell turtles are traditionally exploited for food and have been farm-bred in China since the Spring and Autumn Period, more than 2400 years ago. Currently, the annual production of Pelodiscus amounts to 340,000 t in China alone. Using mitochondrial DNA (2428 bp) and five nuclear loci (3704 bp), we examined broad sampling of wild and farm-bred Pelodiscus to infer genetic and taxonomic differentiation. We discovered four previously unknown mitochondrial lineages, all from China. One lineage from Jiangxi is deeply divergent and sister to the mitochondrial lineage of Pelodiscus axenaria. The nuclear loci supported species status for P. axenaria and the new lineage from Jiangxi. Pelodiscus maackii and P. parviformis, both harboring distinct mitochondrial lineages, were not differentiated from P. sinensis in the studied nuclear markers. The same is true for two new mitochondrial lineages from Zhejiang, China, represented by only one individual each, and another new lineage from Anhui, Guangdong, Jiangxi and Zhejiang, China. However, Vietnamese turtles yielding a mitochondrial lineage clustering within P. sinensis were distinct in nuclear markers, suggesting that these populations could represent another unknown species with introgressed mitochondria. Its species status is also supported by the syntopic occurrence with P. sinensis in northern Vietnam and by morphology. In addition, we confirmed sympatry of P. axenaria and P. parviformis in Guangxi, China, and found evidence for sympatry of P. sinensis and the new putative species from Jiangxi, China. We also discovered evidence for hybridization in turtle farms and for the occurrence of alien lineages in the wild (Zhejiang, China), highlighting the risk of genetic pollution of native stock. In the face of the large-scale breeding of Pelodiscus, we claim that the long-term survival of distinct genetic lineages and species can only be assured when an upscale market segment for pure-bred softshell turtles is established, making the breeding of pure lineages lucrative for turtle farms. Our findings underline that the diversity of Pelodiscus is currently underestimated and threatened by anthropogenic admixture. We recommend mass screening of genetic and morphological variation of Chinese softshell turtles as a first step to understand and preserve their diversity.

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Twenty microsatellite loci from Chinese soft-shelled Turtles Trionyx sinensis, a vulnerable species on the IUCN Red List

Liu

Wang

et al. 2017

Conservation Genet Resour

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Development and characterization of 17 microsatellite markers for Chinese soft-shelled turtle, Pelodiscus sinensis

Cited by 3 publications

References 3 publications

Short Communication Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

Short Communication Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

Millennium-old farm breeding of Chinese softshell turtles (Pelodiscus spp.) results in massive erosion of biodiversity

Twenty microsatellite loci from Chinese soft-shelled Turtles Trionyx sinensis, a vulnerable species on the IUCN Red List

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