Development and characterization of a bacteriophage cocktail with high lytic efficacy against field-isolated Salmonella enterica

Aguilera, Matías; Tobar-Calfucoy, Eduardo; Rojas-Martínez, Victoria; Norambuena, Rodrigo; Serrano, María Jesús; Cifuentes, Onix; Zamudio, María Sofía; San Martín, Daniel; Lara, Pabla; Sabag, Andrea; Zabner, Marcela; Tichy, Daniel; Camejo, Pamela; León, Luis; Pino, Michael; Ulloa, Soledad; Rojas, Felipe; Pieringer, Christian; Muster, Cecilia; Castillo, Daniel; Ferreira, Nicolás; Avendaño, Camilo; Canaval, Mauro; Pieringer, Hans; Cifuentes, Pablo; Cifuentes Muñoz, Nicolás

doi:10.1016/j.psj.2023.103125

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…One of the main issues regarding phage therapy is the possible emergence of phage-resistant bacterial variants, which could impede favorable outcomes on a long-term basis ( Oechslin, 2018 ). To reduce the effects such variants, have on phage therapy, in-depth characterizations of the bacteriophages and cocktails of different phages should be carried ( Leskinen et al, 2017 ; Aguilera et al, 2023 ). Nevertheless, the isolation of AP from poultry farms, could also bypass this issue, since the simultaneous presence of both bacteria and phage in the same environment for long periods of time leads to co-evolution dynamics ( Olszak et al, 2017 ; Sevilla-Navarro et al, 2018 ; Torres-Boncompte et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

Sevilla-Navarro,

Torres-Boncompte,

Garcia-Llorens

et al. 2024

Front. Microbiol.

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IntroductionSalmonella is a bacterium that can cause food-borne infections and is responsible for the most common gastrointestinal illnesses. The emergence of multi-drug resistant (MDR) strains worldwide is a major threat, representing a major challenge in public health. To reduce its incidence, the One Health approach is required, and the development of new biocontrol protocols will help prevent or eliminate the spread of Salmonella. Prevention measures, such as on-farm cleaning and disinfection protocols, are a crucial step in reducing infection to new flocks and eliminating bacteria that remain in the facilities. However, MDR Salmonella species, such as S. Infantis, are highly resistant to conventional cleaning and disinfection protocols, with an increased ability to persist in the broiler farm environment. The need for alternative biocontrol methods has led to the use of bacteriophages or phages, viruses that target bacteria, as promising tools. Thus, the aim of this study was to evaluate the efficacy of phages as a biocide against S. Infantis isolates in combination with cleaning and disinfection protocols in 10 commercial poultry farms.MethodsAll commercial farms selected in this study had persistent Salmonella, even after the routinely used cleaning and disinfection procedures. In addition, Salmonella isolated before treatment were phenotypically characterized by antimicrobial resistance patterns.ResultsThe results showed that 100% of S. Infantis were resistant to at least one antibiotic, and > 70% were MDR. Phages were then isolated against the in-farm bacteria, purified, and multiplied for each poultry farm. The cleaning and disinfection protocols included the application of the lytic phages (vB_Si_CECAV_FGS009; vB_Si_CECAV_FGS017; vB_Si_CECAV_FGS029 and vB_Si_CECAV _FGS030) twice at 24-h intervals between cleaning and disinfection. Following the cleaning and disinfection procedures, Salmonella detection was reduced from 100% after cleaning to 36% after applying the phages and dropped to 0% after the final step of disinfection, thus eliminating Salmonella from the farm facilities.DiscussionThis study demonstrates that bacteriophage application after cleaning and before disinfection enhances the removal of MDR Salmonella Infantis in commercial broiler farms, suggesting their use as biocontrol agents to reduce Salmonella, a major public health concern.

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Section: Discussionmentioning

confidence: 99%

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

Sevilla-Navarro,

Torres-Boncompte,

Garcia-Llorens

et al. 2024

Front. Microbiol.

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Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product

Pino,

Mujica,

Mora-Uribe

et al. 2025

Poultry Science

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Application of bacteriophages in biopolymer‐based functional food packaging films

Rindhe,

Khan,

Priyadarshi

et al. 2024

Comp Rev Food Sci Food Safe

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Recently, food spoilage caused by pathogens has been increasing. Therefore, applying control strategies is essential. Bacteriophages can potentially reduce this problem due to their host specificity, ability to inhibit bacterial growth, and extend the shelf life of food. When bacteriophages are applied directly to food, their antibacterial activity is lost. In this regard, bacteriophage‐loaded biopolymers offer an excellent option to improve food safety by extending their shelf life. Applying bacteriophages in food preservation requires comprehensive and structured information on their isolation, culturing, storage, and encapsulation in biopolymers for active food packaging applications. This review focuses on using bacteriophages in food packaging and preservation. It discusses the methods for phage application on food, their use for polymer formulation and functionalization, and their effect in enhancing food matrix properties to obtain maximum antibacterial activity in food model systems.

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Development and characterization of a bacteriophage cocktail with high lytic efficacy against field-isolated Salmonella enterica

Cited by 3 publications

References 61 publications

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product

Application of bacteriophages in biopolymer‐based functional food packaging films

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