Development and characterization of a hydrogen peroxide-resistant cholangiocyte cell line: A novel model of oxidative stress-related cholangiocarcinoma genesis

Thanan, Raynoo; Techasen, Anchalee; Hou, Bo; Jamnongkan, Wassana; Armartmuntree, Napat; Yongvanit, Puangrat; Murata, Mariko

doi:10.1016/j.bbrc.2015.06.112

Cited by 23 publications

(35 citation statements)

References 30 publications

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“…Gestational diabetes exposes the fetus to hyperglycemia-induced oxidative stress resulting in increased Pax3 CpG island methylation by promoting DNMT3b activity (108). Similarly, cholangiocarcinoma liver cancer cells achieved resistance to oxidative stress by increasing expression levels of epigenetics-related genes such as DNMT1 (109). These results indicate that oxidative stress downregulates expression of specific genes by promoting DNMT activity, thereby providing a molecular link between oxidative stress and epigenetic gene regulation.…”

Section: Mechanism: Link Between Oxidative Stress and Epigenetic Genementioning

confidence: 61%

Low-Dose Ionizing Radiation Exposure, Oxidative Stress and Epigenetic Programing of Health and Disease

et al. 2017

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Section: Mechanism: Link Between Oxidative Stress and Epigenetic Genementioning

confidence: 61%

Low-Dose Ionizing Radiation Exposure, Oxidative Stress and Epigenetic Programing of Health and Disease

et al. 2017

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“…Some cells can survive by adapting to oxidative stress conditions, and selective clonal expansion of these resistant cells would be involved in oxidative stress–related carcinogenesis. Thanan et al [ 210 ] generated an H 2 O 2 -resistant cell line from an immortal cholangiocyte cell line (MMNK1) by chronic treatment with low-concentration H 2 O 2 . The resistant ox-MMNK1-L cell line showed H 2 O 2 -resistant properties, increasing the expression of the anti-oxidant genes catalase ( CAT ), superoxide dismutase-1 ( SOD1 ), superoxide dismutase-2 ( SOD2 ), and superoxide dismutase-3 ( SOD3 ) and the enzyme activities of CAT and intracellular SODs.…”

Section: Epigenetics Of Drug Resistancementioning

confidence: 99%

Epigenetics of Aging and Alzheimer’s Disease: Implications for Pharmacogenomics and Drug Response

Cacabelos

Torrellas

2015

IJMS

109

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Epigenetic variability (DNA methylation/demethylation, histone modifications, microRNA regulation) is common in physiological and pathological conditions. Epigenetic alterations are present in different tissues along the aging process and in neurodegenerative disorders, such as Alzheimer’s disease (AD). Epigenetics affect life span and longevity. AD-related genes exhibit epigenetic changes, indicating that epigenetics might exert a pathogenic role in dementia. Epigenetic modifications are reversible and can potentially be targeted by pharmacological intervention. Epigenetic drugs may be useful for the treatment of major problems of health (e.g., cancer, cardiovascular disorders, brain disorders). The efficacy and safety of these and other medications depend upon the efficiency of the pharmacogenetic process in which different clusters of genes (pathogenic, mechanistic, metabolic, transporter, pleiotropic) are involved. Most of these genes are also under the influence of the epigenetic machinery. The information available on the pharmacoepigenomics of most drugs is very limited; however, growing evidence indicates that epigenetic changes are determinant in the pathogenesis of many medical conditions and in drug response and drug resistance. Consequently, pharmacoepigenetic studies should be incorporated in drug development and personalized treatments.

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“…However, if this repairing process occurs under oxidative stress, it may disturb the stem cell differentiation and consequently contribute to CCA carcinogenesis. Prolonged exposure to oxidative stress is known to involve in tumorigenesis and expression of stem-like cell properties of the immortal cholangiocytes [12,13]. In addition, CCA cells have liver stem/progenitor cells phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Opposing Roles of FoxA1 and FoxA3 in Intrahepatic Cholangiocarcinoma Progression

Thanan

Kaewlert

Sakonsinsiri

et al. 2020

IJMS

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Cholangiocarcinoma (CCA), a malignancy of biliary epithelium, is related to liver stem cell deregulation. FoxAs are a group of transcription factors that play critical roles in liver stem cell differentiation. In this study, the expression levels of FoxAs (i.e., FoxA1, FoxA2 and FoxA3) were detected in intrahepatic CCA tissues and the functions of FoxAs were studied in CCA cell lines. FoxA1 and FoxA2 were mainly localized in the nuclei of normal bile duct (NBD) cells and some of the cancer cells. Low expression of FoxA1 in CCA tissues (72%) was significantly correlated with poor prognosis. FoxA3 expression of CCA cells was localized in the nucleus and cytoplasm, whereas it was slightly detected in NBDs. High expression of FoxA3 in cancer tissues (61%) was significantly related to high metastasis status. These findings suggest the opposing roles of FoxA1 and FoxA3 in CCA. Moreover, the FoxA1-over-expressing CCA cell line exhibited a significant reduction in proliferative and invasive activities compared to control cells. Knockdown of FoxA3 in CCA cells resulted in a significant decrease in proliferative and invasive activities compared with control cells. Taken together, in CCA, FoxA1 is down-regulated and has tumor suppressive roles, whereas FoxA3 is up-regulated and has oncogenic roles.

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Development and characterization of a hydrogen peroxide-resistant cholangiocyte cell line: A novel model of oxidative stress-related cholangiocarcinoma genesis

Cited by 23 publications

References 30 publications

Low-Dose Ionizing Radiation Exposure, Oxidative Stress and Epigenetic Programing of Health and Disease

Low-Dose Ionizing Radiation Exposure, Oxidative Stress and Epigenetic Programing of Health and Disease

Epigenetics of Aging and Alzheimer’s Disease: Implications for Pharmacogenomics and Drug Response

Opposing Roles of FoxA1 and FoxA3 in Intrahepatic Cholangiocarcinoma Progression

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