Development and characterization of a chitosan-supported immunoaffinity chromatography column for the selective extraction of methandrostenolone from food and feed samples

Wang, Yun; Xu, Yan; Zhang, Xun; Wang, Enlan; Dong, Ying

doi:10.1016/j.ijbiomac.2011.05.027

Cited by 15 publications

(15 citation statements)

References 26 publications

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“…The size homogeneity of the chitosan beads prepared in this study is much better than that of chitosan beads obtained in our earlier work using epichlorohydrin as cross-linking reagent that ranged from 20 to 80 mm (Wang et al, 2011).…”

Section: Physicochemical Properties Of Chitosan-based Immunoaffinity contrasting

confidence: 60%

“…According to Wang et al (2011), the chromatographic performance of the column was characterised in terms of its eluting condition, binding capacity, specificity, reproducibility and reusability.…”

Section: Characterisation Of the Chromatographic Performances Of The mentioning

confidence: 99%

“…IAC has been successfully used for the clean-up of various analytes, including mycotoxins, veterinary drugs, pesticides, vitamins and heavy metals, in a large range of different matrices (Abe et al, 2011;Chuang, Van Emon, Jones, Durnford, & Lordo, 2007;Heudi, Kilinç, Fontannaz, & Marley, 2006;Holtzapple, Buckley, & Stanker, 2001;Meletis, Meniades-Meimaroglou, & Markaki, 2007;Wang & Shen, 2007). A preliminary preparation of a chitosan-based immunoaffinity adsorbent using epichlorohydrin as a cross-linking reagent has been described in an earlier work of ours (Wang, Xu, Zhang, Wang, & Dong, 2011). However, the prepared immunoaffinity adsorbent was inadequate in size uniformity of the chitosan bead and stability to repeated use.…”

Section: Introductionmentioning

confidence: 99%

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Improved preparation of a chitosan-based immunoaffinity column using antibody against methandrostenolone as ligand

Wang

et al. 2013

Food and Agricultural Immunology

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Chitosan is obtained by N-deacetylation of chitin, which is the second most abundant natural biopolymer. In this study, an improved preparation and characterisation of a chitosan-based immunoaffinity chromatography (IAC) column were performed. The immunoaffinity adsorbent was prepared by covalently coupling monoclonal antibody (mAb) against methandrostenolone (MA) to glutaraldehyde cross-linked chitosan (chitosan CL). Scanning electron micrograph of chitosan CL indicated that the shape of the particles was spherical with a diameter range from about 300 to 500 mm. Infrared spectral analysis suggested that the immunoaffinity adsorbent was successfully prepared by coupling antibody with chitosan CL. For chromatographic extraction, 90% methanol was selected as eluant. Under optimum conditions, the maximum binding capacity of the IAC column was 3900 ng MA/mL adsorbent. The average recovery of 50, 250 and 500 ng of MA standards from IAC columns was 96.9% with a relative standard deviation among columns of 1.48%. After eight uses, the extraction recovery of the IAC column remained 82.1%. To further verify the effect of matrices on the extraction efficiency, the IAC columns were challenged with MA-fortified animal tissue and feed samples. Recoveries were 84.9Á87.1%, demonstrating the effectiveness of these IAC columns for sample clean-up in MA residue determination.

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Section: Physicochemical Properties Of Chitosan-based Immunoaffinity contrasting

confidence: 60%

Section: Characterisation Of the Chromatographic Performances Of The mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improved preparation of a chitosan-based immunoaffinity column using antibody against methandrostenolone as ligand

Wang

et al. 2013

Food and Agricultural Immunology

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“…The preparation of haptens and conjugates and the generation of anti-MA mAbs were conducted according to our previous reports (Wang et al, 2011a;Wang, Xu, Zhang, Wang, & Dong, 2011b). In brief, a carboxyl group was introduced into MA by oximate reaction firstly and then the synthetic hapten MA (Figure 1) was covalently coupled with keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), respectively, using the modified active ester method to obtain immunogens and coating antigens.…”

Section: Preparation and Evaluation Of Monoclonal Anti-ma Antibodiesmentioning

confidence: 99%

“…The MA-BSA was used as a coating antigen, and ELISA was performed on a 96-well polystyrene microtitre plate as described in our previously reported study (Wang et al, 2011b). A set of MA solution (0, 0.01, 0.05, 0.25, 1.25, 6.25, 31.25, 156.25 and 781.25 ng/mL) was analysed by the developed ELISA method.…”

Section: Elisa Proceduresmentioning

confidence: 99%

Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples

Wang

Zhang

et al. 2013

Food and Agricultural Immunology

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2013)Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples, Food and Agricultural Immunology, 24:2, 231-240, Methandrostenolone (MA) is used as veterinary medicine for promoting foodproducing animal growth. Accumulation of MA in a human body would cause multiple side effects. In this study, an anti-MA monoclonal antibody (mAb)-based immunoaffinity column (IAC) was prepared and its application to the selective extraction of MA from actual samples was evaluated. The employed antibody exhibited good sensitivity and specificity towards MA with an IC 50 value of 7.89 ng/mL and less than 1% cross-reactivity towards structurally related compounds. By coupling CNBr-activated Sepharose with the antibody, an IAC was prepared. Two percent methanol and 70% methanol were selected, respectively, as loading and eluting solution by optimisation. The maximum binding capacity of the column for MA reached 4760 ng/mL gel. The average recovery of 50, 250 and 500 ng MA standards from IACs was 88.6% with a relative standard deviation (RSD) among columns of 8.5%. After three times usage, the binding capacity and recovery still remained 93.4% and 81.1%, respectively. To further verify the effect of matrices on the extraction efficiency, the IACs were challenged with MA-fortified animal tissue and feed samples and the recovery were found to be in the range of 78.7%Á95.1%.

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Immunosorbent Materials in Chromatography

Rodriguez

Poddar

Hage

2019

Handbook of Smart Materials in Analytical Chemistry

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Development and characterization of a chitosan-supported immunoaffinity chromatography column for the selective extraction of methandrostenolone from food and feed samples

Cited by 15 publications

References 26 publications

Improved preparation of a chitosan-based immunoaffinity column using antibody against methandrostenolone as ligand

Improved preparation of a chitosan-based immunoaffinity column using antibody against methandrostenolone as ligand

Development and characterisation of an immunoaffinity column for the selective extraction of methandrostenolone from food and feed samples

Immunosorbent Materials in Chromatography

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