Development and characterization of microsatellite markers for the Brazil nut tree <i>Bertholletia excelsa</i> Humb. &amp; Bonpl. (Lecythidaceae)

Reis, Alessandra Maria Moreira; Braga, Aline Cabral; Lemes, Maristerra R.; Gribel, Rogério; Collevatti, Rosane Garcia

doi:10.1111/j.1755-0998.2008.02481.x

Cited by 19 publications

(22 citation statements)

References 14 publications

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“…This is the first study which compares the tocopherol content in Brazil nuts from different geographic areas. More comprehensive studies would also be of interest, such as those pertaining to variation in samplings acquired over several years from the same location, seasonal effects (early and late harvests), and the impact of genotype (Reis et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Tocopherol profile of Brazil nut oil from different geographic areas of the Amazon region

et al. 2013

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The tocopherol content of Brazil nut oil from different Amazon regions (Manicoré-AM, Rio Preto da Eva-AM, São João da Baliza-RR, Caroebe-RR, Belém-PA, and Xapurí-AC) was investigated by normal-phase high-performance liquid chromatography. For all authentic oils, two isomers: a-and g-tocopherols were observed (37.92-74.48 mg g , respectively), and their levels were relatively constant among the oils having these geographic origins, which would enable to distinguish Brazil nut oil from other plant oils for authentication purposes. Commercial Brazil nut oils were also evaluated, and some of these oils demonstrated a tocopherol content that was very different from that of the authentic oils. Therefore, we suggest that the tocopherol profile of Brazil nut oil can be useful chemical marker for quality control and authentication. KEYWORDS: Bertholletia excelsa H.B.K., Amazonian vegetable oil, quality control, authenticity. Perfil de tocoferol de castanha-do-Brasil de diferentes áreas geográficas da região Amazônica RESUMOO teor de tocoferol de óleo de castanha-do-Brasil proveniente de diferentes regiões da Amazônia (Manicoré-AM, Rio Preto da Eva-AM, São João da Baliza-RR, Caroebe-RR, Belém-PA e Xapurí-AC) foi investigado através de cromatografia líquida de alta eficiência em fase normal. Em todos os óleos autênticos, dois isômeros, a-e g-tocoferóis (37,92-74,48 mg g , respectivamente), foram observados, e seus níveis foram relativamente constantes em todos os óleos destas origens geográficas, o que pode distinguir o óleo de castanha-do-Brasil dos óleos de outras plantas, em termos de autenticidade. Os óleos comerciais de castanha-do-Brasil também foram avaliados e alguns mostraram teor de tocoferol muito diferente que o dos óleos autênticos. Portanto, sugere-se que o perfil de tocoferol do óleo de castanha-do-Brasil pode ser usado como marcador químico para controle de qualidade e autenticação. PALAVRAS-CHAVE: Bertholletia excelsa H.B.K., óleo vegetal da Amazônia, controle de qualidade, autenticidade.

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Section: Discussionmentioning

confidence: 99%

Tocopherol profile of Brazil nut oil from different geographic areas of the Amazon region

et al. 2013

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“…Individuals were genotyped with eleven microsatellite markers, seven of which were developed by Sujii et al (2013), and four developed by Reis et al (2009). Both the conditions of amplification reactions and the characterization of loci are as described previously (Sujii et al 2013).…”

Section: Microsatellite Genotypingmentioning

confidence: 99%

Genetic structure of Bertholletia excelsa populations from the Amazon at different spatial scales

Sujii

Martins

Wadt³

et al. 2015

Conserv Genet

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Population genetic structure and genetic diversity levels are important issues to understand population dynamics and to guide forest management plans. The Brazil nut tree (Bertholletia excelsa Bonpl.) is an endemic species, widely distributed through Amazonian upland forests and also an important species for the local extractive economy. Our aim was to analyze the genetic structure of Brazil nut trees at both fine and large scales throughout the Amazon Basin, contributing to the knowledge base on this species and to generate information to support plans for its conservation. We genotyped individuals from nine sites distributed in five regions of the Brazilian Amazon using 11 microsatellite loci. We found an excess of heterozygotes in most populations, with significant negative inbreeding coefficients (f) for five of them and the finescale structure, when present, was very small. These results, as a consequence of self-incompatibility, indicate that conservation plans for B. excelsa must include the maintenance of genetic diversity within populations to ensure viable amounts of seeds for both economic purposes and for the local persistence of the species.

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“…Despite the usefulness of microsatellite makers, there are few reports on the development, characterization and use of microsatellite loci in Neotropical tree species (Collevatti et al, 1999;Braga et al, 2007;Reis et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

Telles¹,

Peixoto²,

Lima³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in "cerrado rupestre" from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic. Among the polymorphic loci, expected heterozygosities ranged from 0.161 to 0.714. Combined paternity exclusion probability was 0.957 and combined genetic identity (0.051) was high for studies on parentage. Tibouchina papyrus is a rare and endemic tree species of outcrop quartzite and sandstone soils, with highly isolated populations, which may have lead to the low degree of polymorphism that we detected. Also, motifs of most loci are larger than dinucleotide, which typically display lower levels of polymorphism.

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Development and characterization of microsatellite markers for the Brazil nut tree Bertholletia excelsa Humb. & Bonpl. (Lecythidaceae)

Cited by 19 publications

References 14 publications

Tocopherol profile of Brazil nut oil from different geographic areas of the Amazon region

Tocopherol profile of Brazil nut oil from different geographic areas of the Amazon region

Genetic structure of Bertholletia excelsa populations from the Amazon at different spatial scales

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

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