Development and characterization of microsatellite markers in the earthworm Drawida gisti Michaelsen, 1931 and cross-amplification in two other congeners

Liu, Hongyi; Wang, G.; Chen, Jie; Zhang, Qingzheng; Ruan, Honghua

doi:10.1007/s11033-020-05799-4

Cited by 3 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cross-species amplification tests revealed that the presented markers were usable for M. guillelmi . The results of cross-species amplification tests may vary for different families, which can be successfully amplified in Moniligastridae and Megascolecidae, but not in Lumbricus ( Liu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Metaphire vulgaris Based on the Mitochondrial COI Gene and Microsatellites

Chen

Ruan

et al. 2021

Front. Genet.

Self Cite

View full text Add to dashboard Cite

The earthworm species Metaphire vulgaris (a member of the Clitellata class) is widely distributed across China, and has important ecological functions and medicinal value. However, investigations into its genetic diversity and differentiation are scarce. Consequently, we evaluated the genetic diversity of five populations of M. vulgaris (GM, HD, NYYZ, QDDY, and QDY) in Yancheng, China via the mitochondrial COI gene and the novel microsatellites developed there. A total of nine haplotypes were obtained by sequencing the mitochondrial COI gene, among which NYYZ and QDDY populations had the greatest number of haplotypes (nh = 5). Further, the nucleotide diversity ranged from 0.00437 to 0.1243. The neighbor-joining trees and the TCS network of haplotypes indicated that earthworm populations within close geographical range were not genetically isolated at these small scale distances. Results of the identification of microsatellite molecular markers revealed that the allele number in 12 microsatellite loci ranged from 4 to 13. The observed heterozygosity ranged from 0.151 to 0.644, whereas the expected heterozygosity ranged from 0.213 to 0.847. The polymorphism data content of most sites was >0.5, which indicated that the designed sites had high polymorphism. Structural analysis results indicated that GM, HD, and NYYZ had similar genetic structures across the five populations. The Nei’s genetic distance between HD and NYYZ populations was the smallest (Ds = 0.0624), whereas that between HD and QDY populations was the largest (Ds = 0.2364). The UPGMA tree showed that HD were initially grouped with NYYZ, followed by GM, and then with QDDY. Furthermore, cross-species amplification tests were conducted for Metaphire guillelmi, which indicated that the presented markers were usable for this species. This study comprised a preliminary study on the genetic diversity of M. vulgaris, which provides basic data for future investigations into this species.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Metaphire vulgaris Based on the Mitochondrial COI Gene and Microsatellites

Chen

Ruan

et al. 2021

Front. Genet.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In other taxa, Masran and Majid (2018) developed seven microsatellite markers from Cimex hemipterus (Fabricius) (Hemiptera: Cimicidae) by analyzing results from H-WE, LD, and screening for null alleles on their natural populations. Recent SSR characterization isolated from fin whales ( Tardy et al 2020 ) and earthworm ( Liu et al 2020 ) assessed H-WE, LD, allelic diversity, heterozygosities, and cross-amplification with other species. These studies ( Keyghobadi et al 2004 , Edillo et al 2007 , Slotman et al 2007 , Lather et al 2015 , Masran and Majid 2018 , Liu et al 2020 , Tardy et al 2020 ) did not necessarily compare between newly characterized microsatellite markers with a set of previously good published markers and those datasets of published markers with problematic loci like that of Brown et al (2011) for Ae.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti has been primarily studied on its evolution with humans owing to its public health consequences ( Brown et al 2011 ). Due to rapid geographic spreading of Aedes vectors brought about by climate change ( Liu-Helmersson et al 2016 ), the global community is now facing increased public health concerns and expanded socioeconomic burden caused by their associated diseases ( Paixao et al 2018 , Liu et al 2020 ).…”

mentioning

confidence: 99%

Novel and Broadly Applicable Microsatellite Markers in Identified Chromosomes of the Philippine Dengue Mosquitoes, Aedes aegypti (Diptera: Culicidae)

Labiros

Catalig

Ymbong

et al. 2022

Journal of Medical Entomology

View full text Add to dashboard Cite

Dengue is the leading arboviral infection in the Philippines. Its endemicity in the country is due to the presence of its primary mosquito vector, Aedes aegypti (L.). This species has limited microsatellite markers. This study characterized microsatellite markers screened in silico from intergenic regions of the updated reference genome of Ae. aegypti from Liverpool, U.K. Criteria for good markers are: polymorphic, inherited in a Mendelian codominant manner, no null alleles, selectively neutral, randomly associated, and broadly applicable across different regions. Genotypes were scored using ABI Peak Scanner and were screened for the presence of null alleles. Hardy-Weinberg equilibrium, linkage disequilibrium, and robustness of the markers were determined by GENEPOP using Ae. aegypti samples from selected highland and lowland sites (n = 30 each) in the Philippines and outgroups (Thailand and Vietnam). Mendelian codominant inheritance was examined using F1 offspring of Ae. aegypti family (n = 30 each) derived from samples collected from Cebu city highlands and Maramag, Bukidnon. From the 63 randomly selected markers, nine were polymorphic. Two markers (Aaeg1-3D of chromosome 1 and Aaeg3-4C of chromosome 3) satisfied all criteria, hence, are good broadly useful microsatellite markers. Two other markers (Aaeg2-2E of chromosome 2 and Aaeg3-2A of chromosome 3) met all criteria but deviated from Mendelian codominant inheritance. These new markers of the Philippine Ae. aegypti with their chromosomal locations relative to the other published markers are presented, and will ultimately be useful in a variety of population genetic studies of Ae. aegypti to protect the public health.

show abstract

“…Polymorphic microsatellite markers have been developed for several earthworm species, including Aporrectodea longa [13], Aporrectodea icterica [14,15], Allolobophora chlorotica [16,17], Hormogaster elisae [18], Dendrobaena octaedra [11], Amynthas cortices [19], several Lumbricus sp. [12,[20][21][22][23][24][25], Drawida gisti [26], and Eisenia fetida [27], but not for E. andrei.…”

Section: Introductionmentioning

confidence: 87%

Polymorphic microsatellite markers demonstrate hybridization and interspecific gene flow between lumbricid earthworm species, Eisenia andrei and E. fetida

et al. 2022

View full text Add to dashboard Cite

The lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) have been used as model organisms for studies on hybridization. Previously they have been identified by species specific sequences of the mitochondrial COI gene of maternal origin (‘a’ or ‘f’) and the nuclear 28S gene of maternal/paternal origin (‘A’ or ‘F’). In experimental crosses, these hermaphroditic species produce progeny of genotypes Ea (aAA), Ef (fFF) and hybrids (aAF and fFA) originating by self-fertilization or cross-fertilization. To facilitate studies on new aspects of the breeding biology and hybridization of earthworms, polymorphic microsatellite markers were developed based on 12 Ea and 12 Ef specimens and validated on DNA samples extracted from 24 genotyped specimens (aAA, fFF, aAF and fFA) from three laboratory-raised families and 10 of them were applied in the present study. The results indicate that microsatellite markers are valuable tools for tracking interspecific gene flow between these species.

show abstract

Development and characterization of microsatellite markers in the earthworm Drawida gisti Michaelsen, 1931 and cross-amplification in two other congeners

Cited by 3 publications

References 24 publications

Genetic Diversity and Population Structure of Metaphire vulgaris Based on the Mitochondrial COI Gene and Microsatellites

Genetic Diversity and Population Structure of Metaphire vulgaris Based on the Mitochondrial COI Gene and Microsatellites

Novel and Broadly Applicable Microsatellite Markers in Identified Chromosomes of the Philippine Dengue Mosquitoes, Aedes aegypti (Diptera: Culicidae)

Polymorphic microsatellite markers demonstrate hybridization and interspecific gene flow between lumbricid earthworm species, Eisenia andrei and E. fetida

Contact Info

Product

Resources

About